Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

Monique Mancuso, Rubén Avendaño-Herrera, R. Zaccone, Alicia E. Toranzo, Beatriz Magariños

Resultado de la investigación: Contribución a una revistaArtículo

10 Citas (Scopus)

Resumen

This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.

Idioma originalInglés
Páginas (desde-hasta)85-92
Número de páginas8
PublicaciónBiological Research
Volumen40
N.º1
DOI
EstadoPublicada - 1 ene 2007

Áreas temáticas de ASJC Scopus

  • Bioquímica, genética y biología molecular (todo)
  • Agricultura y biología (todo)

Huella Profundice en los temas de investigación de 'Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida'. En conjunto forman una huella única.

  • Citar esto