Evaluation of cations and chelating agents as extracellular extractants for Cu, Pb, V and Zn in the sequential elution technique applied to the terrestrial moss Pseudoscleropodium purum

Alicia Pérez-Llamazares, Cristóbal J. Galbán-Malagón, Jesús R. Aboal, J. Ángel Fernández, Alejo Carballeira

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

Three experiments were carried out to select the best extractant for use in the sequential elution technique, to enable extraction of Cu, Pb, V and Zn from the extracellular fraction of the terrestrial moss Pseudoscleropodium purum. The optimal concentrations of the extractants tested (CoCl2, NiCl2, Pb(NO3)2, SrCl2, dimercaprol, EDTA, penicillamine) were determined on the basis of the maximum extraction of Zn achieved without any alteration of the plasma membrane. The capacity of these agents (at the optimal concentrations established) to extract the extracellular fractions of Cu, Pb, V and Zn was then evaluated. Extraction with 10mM EDTA is recommended for all 4 elements considered. As a second option, the use of 50mM penicillamine is recommended to extract Cu, 30mM dimercaprol to extract Pb and V and 20mM NiCl2 to extract Zn. It was also concluded that these results cannot be extrapolated to other cryptogams, and that separate assays are required.

Idioma originalEnglish
Páginas (desde-hasta)507-514
Número de páginas8
PublicaciónEcotoxicology and Environmental Safety
Volumen73
N.º4
DOI
EstadoPublished - 1 may 2010

Huella dactilar

Bryophyta
Dimercaprol
Penicillamine
Chelating Agents
Chelation
Edetic Acid
Cations
Positive ions
Ethylenediaminetetraacetic acid
Cell Membrane
Cell membranes
Assays
Experiments

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Citar esto

Pérez-Llamazares, Alicia ; Galbán-Malagón, Cristóbal J. ; Aboal, Jesús R. ; Ángel Fernández, J. ; Carballeira, Alejo. / Evaluation of cations and chelating agents as extracellular extractants for Cu, Pb, V and Zn in the sequential elution technique applied to the terrestrial moss Pseudoscleropodium purum. En: Ecotoxicology and Environmental Safety. 2010 ; Vol. 73, N.º 4. pp. 507-514.
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Evaluation of cations and chelating agents as extracellular extractants for Cu, Pb, V and Zn in the sequential elution technique applied to the terrestrial moss Pseudoscleropodium purum. / Pérez-Llamazares, Alicia; Galbán-Malagón, Cristóbal J.; Aboal, Jesús R.; Ángel Fernández, J.; Carballeira, Alejo.

En: Ecotoxicology and Environmental Safety, Vol. 73, N.º 4, 01.05.2010, p. 507-514.

Resultado de la investigación: Article

TY - JOUR

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AU - Pérez-Llamazares, Alicia

AU - Galbán-Malagón, Cristóbal J.

AU - Aboal, Jesús R.

AU - Ángel Fernández, J.

AU - Carballeira, Alejo

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N2 - Three experiments were carried out to select the best extractant for use in the sequential elution technique, to enable extraction of Cu, Pb, V and Zn from the extracellular fraction of the terrestrial moss Pseudoscleropodium purum. The optimal concentrations of the extractants tested (CoCl2, NiCl2, Pb(NO3)2, SrCl2, dimercaprol, EDTA, penicillamine) were determined on the basis of the maximum extraction of Zn achieved without any alteration of the plasma membrane. The capacity of these agents (at the optimal concentrations established) to extract the extracellular fractions of Cu, Pb, V and Zn was then evaluated. Extraction with 10mM EDTA is recommended for all 4 elements considered. As a second option, the use of 50mM penicillamine is recommended to extract Cu, 30mM dimercaprol to extract Pb and V and 20mM NiCl2 to extract Zn. It was also concluded that these results cannot be extrapolated to other cryptogams, and that separate assays are required.

AB - Three experiments were carried out to select the best extractant for use in the sequential elution technique, to enable extraction of Cu, Pb, V and Zn from the extracellular fraction of the terrestrial moss Pseudoscleropodium purum. The optimal concentrations of the extractants tested (CoCl2, NiCl2, Pb(NO3)2, SrCl2, dimercaprol, EDTA, penicillamine) were determined on the basis of the maximum extraction of Zn achieved without any alteration of the plasma membrane. The capacity of these agents (at the optimal concentrations established) to extract the extracellular fractions of Cu, Pb, V and Zn was then evaluated. Extraction with 10mM EDTA is recommended for all 4 elements considered. As a second option, the use of 50mM penicillamine is recommended to extract Cu, 30mM dimercaprol to extract Pb and V and 20mM NiCl2 to extract Zn. It was also concluded that these results cannot be extrapolated to other cryptogams, and that separate assays are required.

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KW - Pollution

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