Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae

Ruben Avendaño-Herrera, Alfredo Molina, Beatriz Magariños, Alicia E. Toranzo, Peter Smith

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

In the present work the M42-A disk diffusion protocols of the Clinical Laboratory Standards Institute were used by two laboratories to provide data on the epidemiological cut-off values for the Streptococcus phocae isolates obtained from diseased Atlantic salmon (Salmo salar) (n=27) and seal (Phoca vitulina) (n=4) to florfenicol (FLO30), erythromycin (ERY15) and oxytetracycline (OTC30). The data generated by the two laboratories for FLO30 zone sizes were normally distributed with a mean of 27.1mm and a standard deviation of 2.5mm, while for ERY15 they had a mean of 25.5mm and a standard deviation of 1.7mm. For FLO30 and ERY15 the wild type cut-off values (COwt) calculated from the data for all 31 strains were ≤20mm for both agents. Neither of the OTC30 data sets from Lab 1 or Lab 2 was normally distributed. Using the data from both laboratories the COwt for OTC30 was ≤21mm and applying this COwt, suggested that 11 strains should be treated as WT and 20 as non wild-type. Of the strains isolated from salmon, the two strains isolated in 1999 and 4 of the six isolated during 2001-2002 were classified, using the COwt of ≤21mm, as WT. Of the 19 strains isolated from 2004 onward, only one, isolated in 2004, was classified as WT. The COwt values, generated by normalized resistance interpretation, can be used as epidemiological cut-off values and have the potential to reduce errors in interpreting susceptibility test data for S. phocae. Moreover, the data generated in this work demonstrated the importance of reducing OTC use for the streptococcal treatment.

Idioma originalEnglish
Páginas (desde-hasta)44-48
Número de páginas5
PublicaciónAquaculture
Volumen314
N.º1-4
DOI
EstadoPublished - 4 abr 2011

Huella dactilar

Streptococcus phocae
Salmo salar
testing
florfenicol
Phoca vitulina
erythromycin
oxytetracycline
seals
salmon
test
laboratory

ASJC Scopus subject areas

  • Aquatic Science

Citar esto

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title = "Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae",
abstract = "In the present work the M42-A disk diffusion protocols of the Clinical Laboratory Standards Institute were used by two laboratories to provide data on the epidemiological cut-off values for the Streptococcus phocae isolates obtained from diseased Atlantic salmon (Salmo salar) (n=27) and seal (Phoca vitulina) (n=4) to florfenicol (FLO30), erythromycin (ERY15) and oxytetracycline (OTC30). The data generated by the two laboratories for FLO30 zone sizes were normally distributed with a mean of 27.1mm and a standard deviation of 2.5mm, while for ERY15 they had a mean of 25.5mm and a standard deviation of 1.7mm. For FLO30 and ERY15 the wild type cut-off values (COwt) calculated from the data for all 31 strains were ≤20mm for both agents. Neither of the OTC30 data sets from Lab 1 or Lab 2 was normally distributed. Using the data from both laboratories the COwt for OTC30 was ≤21mm and applying this COwt, suggested that 11 strains should be treated as WT and 20 as non wild-type. Of the strains isolated from salmon, the two strains isolated in 1999 and 4 of the six isolated during 2001-2002 were classified, using the COwt of ≤21mm, as WT. Of the 19 strains isolated from 2004 onward, only one, isolated in 2004, was classified as WT. The COwt values, generated by normalized resistance interpretation, can be used as epidemiological cut-off values and have the potential to reduce errors in interpreting susceptibility test data for S. phocae. Moreover, the data generated in this work demonstrated the importance of reducing OTC use for the streptococcal treatment.",
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Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae. / Avendaño-Herrera, Ruben; Molina, Alfredo; Magariños, Beatriz; Toranzo, Alicia E.; Smith, Peter.

En: Aquaculture, Vol. 314, N.º 1-4, 04.04.2011, p. 44-48.

Resultado de la investigación: Article

TY - JOUR

T1 - Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae

AU - Avendaño-Herrera, Ruben

AU - Molina, Alfredo

AU - Magariños, Beatriz

AU - Toranzo, Alicia E.

AU - Smith, Peter

PY - 2011/4/4

Y1 - 2011/4/4

N2 - In the present work the M42-A disk diffusion protocols of the Clinical Laboratory Standards Institute were used by two laboratories to provide data on the epidemiological cut-off values for the Streptococcus phocae isolates obtained from diseased Atlantic salmon (Salmo salar) (n=27) and seal (Phoca vitulina) (n=4) to florfenicol (FLO30), erythromycin (ERY15) and oxytetracycline (OTC30). The data generated by the two laboratories for FLO30 zone sizes were normally distributed with a mean of 27.1mm and a standard deviation of 2.5mm, while for ERY15 they had a mean of 25.5mm and a standard deviation of 1.7mm. For FLO30 and ERY15 the wild type cut-off values (COwt) calculated from the data for all 31 strains were ≤20mm for both agents. Neither of the OTC30 data sets from Lab 1 or Lab 2 was normally distributed. Using the data from both laboratories the COwt for OTC30 was ≤21mm and applying this COwt, suggested that 11 strains should be treated as WT and 20 as non wild-type. Of the strains isolated from salmon, the two strains isolated in 1999 and 4 of the six isolated during 2001-2002 were classified, using the COwt of ≤21mm, as WT. Of the 19 strains isolated from 2004 onward, only one, isolated in 2004, was classified as WT. The COwt values, generated by normalized resistance interpretation, can be used as epidemiological cut-off values and have the potential to reduce errors in interpreting susceptibility test data for S. phocae. Moreover, the data generated in this work demonstrated the importance of reducing OTC use for the streptococcal treatment.

AB - In the present work the M42-A disk diffusion protocols of the Clinical Laboratory Standards Institute were used by two laboratories to provide data on the epidemiological cut-off values for the Streptococcus phocae isolates obtained from diseased Atlantic salmon (Salmo salar) (n=27) and seal (Phoca vitulina) (n=4) to florfenicol (FLO30), erythromycin (ERY15) and oxytetracycline (OTC30). The data generated by the two laboratories for FLO30 zone sizes were normally distributed with a mean of 27.1mm and a standard deviation of 2.5mm, while for ERY15 they had a mean of 25.5mm and a standard deviation of 1.7mm. For FLO30 and ERY15 the wild type cut-off values (COwt) calculated from the data for all 31 strains were ≤20mm for both agents. Neither of the OTC30 data sets from Lab 1 or Lab 2 was normally distributed. Using the data from both laboratories the COwt for OTC30 was ≤21mm and applying this COwt, suggested that 11 strains should be treated as WT and 20 as non wild-type. Of the strains isolated from salmon, the two strains isolated in 1999 and 4 of the six isolated during 2001-2002 were classified, using the COwt of ≤21mm, as WT. Of the 19 strains isolated from 2004 onward, only one, isolated in 2004, was classified as WT. The COwt values, generated by normalized resistance interpretation, can be used as epidemiological cut-off values and have the potential to reduce errors in interpreting susceptibility test data for S. phocae. Moreover, the data generated in this work demonstrated the importance of reducing OTC use for the streptococcal treatment.

KW - Antibiotic susceptibility and resistance

KW - Atlantic salmon

KW - Epidemiological cut-off values

KW - Streptococcus phocae

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U2 - 10.1016/j.aquaculture.2011.01.049

DO - 10.1016/j.aquaculture.2011.01.049

M3 - Article

AN - SCOPUS:79952736870

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SP - 44

EP - 48

JO - Aquaculture

JF - Aquaculture

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