Epithelial control of the human pDC response to extracellular bacteria

Paula Michea, Pablo Vargas, Marie Hélène Donnadieu, Mario Rosemblatt, María Rosa Bono, Guillaume Duménil, Vassili Soumelis

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

Plasmacytoid pre-dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs to viruses is well established, whether pDCs can respond to extracellular bacteria remains controversial. Here, we demonstrate that extracellular bacteria such as Neisseria meningitidis, Haemophilus influenzae, and Staphylococcus aureus activate pDCs to produce IFN-α, TNF-α, IL-6, and to upregulate CD86 expression. We observed that pDCs were present within tonsillar crypts and oro-nasopharyngeal epithelium, where they may contact extracellular bacteria, in situ. Tonsil epithelium-conditioned supernatants inhibited IFN-α, TNF-α, and IL-6 triggered by the direct contact of N. meningitidis or S. aureus with pDCs. However, pDC priming of naive T cells was not affected, suggesting that tonsil epithelium micro-environment limits local inflammation while preserving adaptive immunity in response to extracellular bacteria. Our results reveal an important and novel function of pDCs in the initiation of the mucosal innate and adaptive immunity to extracellular bacteria.

Idioma originalEnglish
Páginas (desde-hasta)1264-1273
Número de páginas10
PublicaciónEuropean Journal of Immunology
Volumen43
N.º5
DOI
EstadoPublished - abr 2013

Huella dactilar

Dendritic Cells
Bacteria
Adaptive Immunity
Neisseria meningitidis
Epithelium
Palatine Tonsil
Staphylococcus aureus
Interleukin-6
Mucosal Immunity
Haemophilus influenzae
Innate Immunity
Nucleic Acids
Up-Regulation
Viruses
Inflammation
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Citar esto

Michea, P., Vargas, P., Donnadieu, M. H., Rosemblatt, M., Bono, M. R., Duménil, G., & Soumelis, V. (2013). Epithelial control of the human pDC response to extracellular bacteria. European Journal of Immunology, 43(5), 1264-1273. https://doi.org/10.1002/eji.201242990
Michea, Paula ; Vargas, Pablo ; Donnadieu, Marie Hélène ; Rosemblatt, Mario ; Bono, María Rosa ; Duménil, Guillaume ; Soumelis, Vassili. / Epithelial control of the human pDC response to extracellular bacteria. En: European Journal of Immunology. 2013 ; Vol. 43, N.º 5. pp. 1264-1273.
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abstract = "Plasmacytoid pre-dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs to viruses is well established, whether pDCs can respond to extracellular bacteria remains controversial. Here, we demonstrate that extracellular bacteria such as Neisseria meningitidis, Haemophilus influenzae, and Staphylococcus aureus activate pDCs to produce IFN-α, TNF-α, IL-6, and to upregulate CD86 expression. We observed that pDCs were present within tonsillar crypts and oro-nasopharyngeal epithelium, where they may contact extracellular bacteria, in situ. Tonsil epithelium-conditioned supernatants inhibited IFN-α, TNF-α, and IL-6 triggered by the direct contact of N. meningitidis or S. aureus with pDCs. However, pDC priming of naive T cells was not affected, suggesting that tonsil epithelium micro-environment limits local inflammation while preserving adaptive immunity in response to extracellular bacteria. Our results reveal an important and novel function of pDCs in the initiation of the mucosal innate and adaptive immunity to extracellular bacteria.",
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Michea, P, Vargas, P, Donnadieu, MH, Rosemblatt, M, Bono, MR, Duménil, G & Soumelis, V 2013, 'Epithelial control of the human pDC response to extracellular bacteria', European Journal of Immunology, vol. 43, n.º 5, pp. 1264-1273. https://doi.org/10.1002/eji.201242990

Epithelial control of the human pDC response to extracellular bacteria. / Michea, Paula; Vargas, Pablo; Donnadieu, Marie Hélène; Rosemblatt, Mario; Bono, María Rosa; Duménil, Guillaume; Soumelis, Vassili.

En: European Journal of Immunology, Vol. 43, N.º 5, 04.2013, p. 1264-1273.

Resultado de la investigación: Article

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AU - Michea, Paula

AU - Vargas, Pablo

AU - Donnadieu, Marie Hélène

AU - Rosemblatt, Mario

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AU - Duménil, Guillaume

AU - Soumelis, Vassili

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N2 - Plasmacytoid pre-dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs to viruses is well established, whether pDCs can respond to extracellular bacteria remains controversial. Here, we demonstrate that extracellular bacteria such as Neisseria meningitidis, Haemophilus influenzae, and Staphylococcus aureus activate pDCs to produce IFN-α, TNF-α, IL-6, and to upregulate CD86 expression. We observed that pDCs were present within tonsillar crypts and oro-nasopharyngeal epithelium, where they may contact extracellular bacteria, in situ. Tonsil epithelium-conditioned supernatants inhibited IFN-α, TNF-α, and IL-6 triggered by the direct contact of N. meningitidis or S. aureus with pDCs. However, pDC priming of naive T cells was not affected, suggesting that tonsil epithelium micro-environment limits local inflammation while preserving adaptive immunity in response to extracellular bacteria. Our results reveal an important and novel function of pDCs in the initiation of the mucosal innate and adaptive immunity to extracellular bacteria.

AB - Plasmacytoid pre-dendritic cells (pDCs) are specialized in responding to nucleic acids, and link innate with adaptive immunity. Although the response of pDCs to viruses is well established, whether pDCs can respond to extracellular bacteria remains controversial. Here, we demonstrate that extracellular bacteria such as Neisseria meningitidis, Haemophilus influenzae, and Staphylococcus aureus activate pDCs to produce IFN-α, TNF-α, IL-6, and to upregulate CD86 expression. We observed that pDCs were present within tonsillar crypts and oro-nasopharyngeal epithelium, where they may contact extracellular bacteria, in situ. Tonsil epithelium-conditioned supernatants inhibited IFN-α, TNF-α, and IL-6 triggered by the direct contact of N. meningitidis or S. aureus with pDCs. However, pDC priming of naive T cells was not affected, suggesting that tonsil epithelium micro-environment limits local inflammation while preserving adaptive immunity in response to extracellular bacteria. Our results reveal an important and novel function of pDCs in the initiation of the mucosal innate and adaptive immunity to extracellular bacteria.

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