Environmental acclimatization of the carp modulates the transcription of β-actin

José Sarmiento, Sergio Leal, Claudia Quezada, Gudrun Kausel, Jaime Figueroa, María Inés Vera, Manuel Krauskopf

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.

Idioma originalInglés
Páginas (desde-hasta)223-228
Número de páginas6
PublicaciónJournal of Cellular Biochemistry
Volumen80
N.º2
DOI
EstadoPublicada - 2000

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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