Environmental acclimatization of the carp modulates the transcription of β-actin

José Sarmiento, Sergio Leal, Claudia Quezada, Gudrun Kausel, Jaime Figueroa, María Inés Vera, Manuel Krauskopf

Resultado de la investigación: Article

21 Citas (Scopus)

Resumen

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.

Idioma originalEnglish
Páginas (desde-hasta)223-228
Número de páginas6
PublicaciónJournal of Cellular Biochemistry
Volumen80
N.º2
DOI
EstadoPublished - 2000

Huella dactilar

Carps
Acclimatization
Transcription
Actins
Genes
Essential Genes
In Situ Hybridization
Ecosystem
Fishes
Gene Expression
Gene expression
Polymerase Chain Reaction
Fish
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Sarmiento, José ; Leal, Sergio ; Quezada, Claudia ; Kausel, Gudrun ; Figueroa, Jaime ; Vera, María Inés ; Krauskopf, Manuel. / Environmental acclimatization of the carp modulates the transcription of β-actin. En: Journal of Cellular Biochemistry. 2000 ; Vol. 80, N.º 2. pp. 223-228.
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Sarmiento, J, Leal, S, Quezada, C, Kausel, G, Figueroa, J, Vera, MI & Krauskopf, M 2000, 'Environmental acclimatization of the carp modulates the transcription of β-actin', Journal of Cellular Biochemistry, vol. 80, n.º 2, pp. 223-228. https://doi.org/10.1002/1097-4644(20010201)80:2<223::AID-JCB110>3.0.CO;2-O

Environmental acclimatization of the carp modulates the transcription of β-actin. / Sarmiento, José; Leal, Sergio; Quezada, Claudia; Kausel, Gudrun; Figueroa, Jaime; Vera, María Inés; Krauskopf, Manuel.

En: Journal of Cellular Biochemistry, Vol. 80, N.º 2, 2000, p. 223-228.

Resultado de la investigación: Article

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T1 - Environmental acclimatization of the carp modulates the transcription of β-actin

AU - Sarmiento, José

AU - Leal, Sergio

AU - Quezada, Claudia

AU - Kausel, Gudrun

AU - Figueroa, Jaime

AU - Vera, María Inés

AU - Krauskopf, Manuel

PY - 2000

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KW - Acclimatization

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KW - Fish

KW - Gene expression quantification

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