Resumen
A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 223-228 |
| Número de páginas | 6 |
| Publicación | Journal of Cellular Biochemistry |
| Volumen | 80 |
| N.º | 2 |
| DOI | |
| Estado | Published - 2000 |
Huella dactilar
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Environmental acclimatization of the carp modulates the transcription of β-actin. / Sarmiento, José; Leal, Sergio; Quezada, Claudia; Kausel, Gudrun; Figueroa, Jaime; Vera, María Inés; Krauskopf, Manuel.
En: Journal of Cellular Biochemistry, Vol. 80, N.º 2, 2000, p. 223-228.Resultado de la investigación: Article
TY - JOUR
T1 - Environmental acclimatization of the carp modulates the transcription of β-actin
AU - Sarmiento, José
AU - Leal, Sergio
AU - Quezada, Claudia
AU - Kausel, Gudrun
AU - Figueroa, Jaime
AU - Vera, María Inés
AU - Krauskopf, Manuel
PY - 2000
Y1 - 2000
N2 - A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.
AB - A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.
KW - β-actin transcription
KW - Acclimatization
KW - Carp
KW - Fish
KW - Gene expression quantification
KW - Housekeeping genes
UR - http://www.scopus.com/inward/record.url?scp=0034532539&partnerID=8YFLogxK
U2 - 10.1002/1097-4644(20010201)80:2<223::AID-JCB110>3.0.CO;2-O
DO - 10.1002/1097-4644(20010201)80:2<223::AID-JCB110>3.0.CO;2-O
M3 - Article
C2 - 11074593
AN - SCOPUS:0034532539
VL - 80
SP - 223
EP - 228
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 2
ER -