Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors

Viviana Valdés, José Ignacio Valenzuela, Daniela A. Salas, Matías Jaureguiberry-Bravo, Carolina Otero, Christina Thiede, Christoph F. Schmidt, Andrés Couve

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

In neuronal cells the intracellular trafficking machinery controls the availability of neurotransmitter receptors at the plasma membrane, which is a critical determinant of synaptic strength. Metabotropic γ amino-butyric acid (GABA) type B receptors (GABABRs) are neurotransmitter receptors that modulate synaptic transmission by mediating the slow and prolonged responses to GABA. GABABRs are obligatory heteromers constituted by two subunits, GABABR1 and GABABR2. GABABR1a and GABABR1b are the most abundant subunit variants. GABABR1b is located in the somatodendritic domain whereas GABABR1a is additionally targeted to the axon. Sushi domains located at the N-terminus of GABABR1a constitute the only difference between both variants and are necessary and sufficient for axonal targeting. The precise targeting machinery and the organelles involved in sorting and transport have not been described. Here we demonstrate that GABABRs require the Golgi apparatus for plasma membrane delivery but that axonal sorting and targeting of GABABR1a operate in a pre-Golgi compartment. In the axon GABABR1a subunits are enriched in the endoplasmic reticulum (ER), and their dynamic behavior and colocalization with other secretory organelles like the ER-to-Golgi intermediate compartment (ERGIC) suggest that they employ a local secretory route. The transport of axonal GABABR1a is microtubule-dependent and kinesin-1, a molecular motor of the kinesin family, determines axonal localization. Considering that progression of GABABRs through the secretory pathway is regulated by an ER retention motif our data contribute to understand the role of the axonal ER in non-canonical sorting and targeting of neurotransmitter receptors.

Idioma originalEnglish
Número de artículoe44168
PublicaciónPLoS ONE
Volumen7
N.º8
DOI
EstadoPublished - 27 ago 2012

Huella dactilar

GABA-B Receptors
Kinesin
kinesin
Sorting
Endoplasmic Reticulum
endoplasmic reticulum
sorting
Neurotransmitter Receptor
receptors
Cell membranes
axons
Organelles
Machinery
organelles
Axons
plasma membrane
sushi
Cell Membrane
synaptic transmission
Axonal Transport

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Citar esto

Valdés, V., Valenzuela, J. I., Salas, D. A., Jaureguiberry-Bravo, M., Otero, C., Thiede, C., ... Couve, A. (2012). Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors. PLoS ONE, 7(8), [e44168]. https://doi.org/10.1371/journal.pone.0044168
Valdés, Viviana ; Valenzuela, José Ignacio ; Salas, Daniela A. ; Jaureguiberry-Bravo, Matías ; Otero, Carolina ; Thiede, Christina ; Schmidt, Christoph F. ; Couve, Andrés. / Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors. En: PLoS ONE. 2012 ; Vol. 7, N.º 8.
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Valdés, V, Valenzuela, JI, Salas, DA, Jaureguiberry-Bravo, M, Otero, C, Thiede, C, Schmidt, CF & Couve, A 2012, 'Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors', PLoS ONE, vol. 7, n.º 8, e44168. https://doi.org/10.1371/journal.pone.0044168

Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors. / Valdés, Viviana; Valenzuela, José Ignacio; Salas, Daniela A.; Jaureguiberry-Bravo, Matías; Otero, Carolina; Thiede, Christina; Schmidt, Christoph F.; Couve, Andrés.

En: PLoS ONE, Vol. 7, N.º 8, e44168, 27.08.2012.

Resultado de la investigación: Article

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T1 - Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors

AU - Valdés, Viviana

AU - Valenzuela, José Ignacio

AU - Salas, Daniela A.

AU - Jaureguiberry-Bravo, Matías

AU - Otero, Carolina

AU - Thiede, Christina

AU - Schmidt, Christoph F.

AU - Couve, Andrés

PY - 2012/8/27

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N2 - In neuronal cells the intracellular trafficking machinery controls the availability of neurotransmitter receptors at the plasma membrane, which is a critical determinant of synaptic strength. Metabotropic γ amino-butyric acid (GABA) type B receptors (GABABRs) are neurotransmitter receptors that modulate synaptic transmission by mediating the slow and prolonged responses to GABA. GABABRs are obligatory heteromers constituted by two subunits, GABABR1 and GABABR2. GABABR1a and GABABR1b are the most abundant subunit variants. GABABR1b is located in the somatodendritic domain whereas GABABR1a is additionally targeted to the axon. Sushi domains located at the N-terminus of GABABR1a constitute the only difference between both variants and are necessary and sufficient for axonal targeting. The precise targeting machinery and the organelles involved in sorting and transport have not been described. Here we demonstrate that GABABRs require the Golgi apparatus for plasma membrane delivery but that axonal sorting and targeting of GABABR1a operate in a pre-Golgi compartment. In the axon GABABR1a subunits are enriched in the endoplasmic reticulum (ER), and their dynamic behavior and colocalization with other secretory organelles like the ER-to-Golgi intermediate compartment (ERGIC) suggest that they employ a local secretory route. The transport of axonal GABABR1a is microtubule-dependent and kinesin-1, a molecular motor of the kinesin family, determines axonal localization. Considering that progression of GABABRs through the secretory pathway is regulated by an ER retention motif our data contribute to understand the role of the axonal ER in non-canonical sorting and targeting of neurotransmitter receptors.

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Valdés V, Valenzuela JI, Salas DA, Jaureguiberry-Bravo M, Otero C, Thiede C y otros. Endoplasmic reticulum sorting and kinesin-1 command the targeting of Axonal GABAB receptors. PLoS ONE. 2012 ago 27;7(8). e44168. https://doi.org/10.1371/journal.pone.0044168