Endometrial gene expression reveals compromised progesterone signaling in women refractory to embryo implantation

Alejandro Tapia-Pizarro, Paula Figueroa, Julio Brito, Juan C. Marín, David J. Munroe, Horacio B. Croxatto

Resultado de la investigación: Contribución a la publicaciónArticle

  • 8 Citas

Resumen

Background: Endometrial function is essential for embryo implantation. The aim of this study was to analyze gene expression profiles from individual endometrial samples obtained from women with repeated implantation failure after IVF in oocyte donation programs. Methods: Seventeen volunteers were recruited: women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group, n = 5) or had at least one successful cycle (Group B, control group, n = 6) and spontaneously fertile women (Group C, normal fertility group, n = 6). An endometrial cycle was induced with exogenous estradiol (E2) and progesterone (P) and an endometrial sample was collected on the seventh day of P treatment. Results: Transcriptome analysis showed 82 genes with consistent differential gene expression when comparing A vs. B and A vs. C. One hundred transcripts differentially expressed in group A vs. B have been shown to be regulated by P, suggesting compromised P signaling in the endometrium. The P receptor (PR) mutation PROGINS was not detected in women from group A. Semi-quantitation of immunoreactive PRA/B, PRB and Sp1 (a transcription factor related to P signaling) in paraffin-embedded endometrial sections, did not show statistically significant differences amongst groups. However immunostaining glycodelin was significantly decreased in endometrial samples from group A. Conclusions: We conclude that some cases of repeated implantation failure could be associated with an aberrant gene expression profile. Compromised P signaling might be the underlying mechanism for such endometrial gene expression deregulation in women with repeated implantation failure.

IdiomaEnglish
Número de artículo92
PublicaciónReproductive Biology and Endocrinology
Volumen12
Número de edición1
DOI
EstadoPublished - 23 sep 2014

Huella dactilar

Progesterone
Gene Expression
Oocyte Donation
Transcriptome
Sp1 Transcription Factor
Gene Expression Profiling
Menstrual Cycle
Endometrium
Paraffin
Fertility
Volunteers
Estradiol
Control Groups
Mutation
Genes
Therapeutics

Keywords

    ASJC Scopus subject areas

    • Developmental Biology
    • Endocrinology
    • Reproductive Medicine
    • Medicine(all)

    Citar esto

    Tapia-Pizarro, Alejandro ; Figueroa, Paula ; Brito, Julio ; Marín, Juan C. ; Munroe, David J. ; Croxatto, Horacio B./ Endometrial gene expression reveals compromised progesterone signaling in women refractory to embryo implantation. En: Reproductive Biology and Endocrinology. 2014 ; Vol. 12, N.º 1.
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    abstract = "Background: Endometrial function is essential for embryo implantation. The aim of this study was to analyze gene expression profiles from individual endometrial samples obtained from women with repeated implantation failure after IVF in oocyte donation programs. Methods: Seventeen volunteers were recruited: women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group, n = 5) or had at least one successful cycle (Group B, control group, n = 6) and spontaneously fertile women (Group C, normal fertility group, n = 6). An endometrial cycle was induced with exogenous estradiol (E2) and progesterone (P) and an endometrial sample was collected on the seventh day of P treatment. Results: Transcriptome analysis showed 82 genes with consistent differential gene expression when comparing A vs. B and A vs. C. One hundred transcripts differentially expressed in group A vs. B have been shown to be regulated by P, suggesting compromised P signaling in the endometrium. The P receptor (PR) mutation PROGINS was not detected in women from group A. Semi-quantitation of immunoreactive PRA/B, PRB and Sp1 (a transcription factor related to P signaling) in paraffin-embedded endometrial sections, did not show statistically significant differences amongst groups. However immunostaining glycodelin was significantly decreased in endometrial samples from group A. Conclusions: We conclude that some cases of repeated implantation failure could be associated with an aberrant gene expression profile. Compromised P signaling might be the underlying mechanism for such endometrial gene expression deregulation in women with repeated implantation failure.",
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    Endometrial gene expression reveals compromised progesterone signaling in women refractory to embryo implantation. / Tapia-Pizarro, Alejandro; Figueroa, Paula; Brito, Julio; Marín, Juan C.; Munroe, David J.; Croxatto, Horacio B.

    En: Reproductive Biology and Endocrinology, Vol. 12, N.º 1, 92, 23.09.2014.

    Resultado de la investigación: Contribución a la publicaciónArticle

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    T1 - Endometrial gene expression reveals compromised progesterone signaling in women refractory to embryo implantation

    AU - Tapia-Pizarro,Alejandro

    AU - Figueroa,Paula

    AU - Brito,Julio

    AU - Marín,Juan C.

    AU - Munroe,David J.

    AU - Croxatto,Horacio B.

    PY - 2014/9/23

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    N2 - Background: Endometrial function is essential for embryo implantation. The aim of this study was to analyze gene expression profiles from individual endometrial samples obtained from women with repeated implantation failure after IVF in oocyte donation programs. Methods: Seventeen volunteers were recruited: women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group, n = 5) or had at least one successful cycle (Group B, control group, n = 6) and spontaneously fertile women (Group C, normal fertility group, n = 6). An endometrial cycle was induced with exogenous estradiol (E2) and progesterone (P) and an endometrial sample was collected on the seventh day of P treatment. Results: Transcriptome analysis showed 82 genes with consistent differential gene expression when comparing A vs. B and A vs. C. One hundred transcripts differentially expressed in group A vs. B have been shown to be regulated by P, suggesting compromised P signaling in the endometrium. The P receptor (PR) mutation PROGINS was not detected in women from group A. Semi-quantitation of immunoreactive PRA/B, PRB and Sp1 (a transcription factor related to P signaling) in paraffin-embedded endometrial sections, did not show statistically significant differences amongst groups. However immunostaining glycodelin was significantly decreased in endometrial samples from group A. Conclusions: We conclude that some cases of repeated implantation failure could be associated with an aberrant gene expression profile. Compromised P signaling might be the underlying mechanism for such endometrial gene expression deregulation in women with repeated implantation failure.

    AB - Background: Endometrial function is essential for embryo implantation. The aim of this study was to analyze gene expression profiles from individual endometrial samples obtained from women with repeated implantation failure after IVF in oocyte donation programs. Methods: Seventeen volunteers were recruited: women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group, n = 5) or had at least one successful cycle (Group B, control group, n = 6) and spontaneously fertile women (Group C, normal fertility group, n = 6). An endometrial cycle was induced with exogenous estradiol (E2) and progesterone (P) and an endometrial sample was collected on the seventh day of P treatment. Results: Transcriptome analysis showed 82 genes with consistent differential gene expression when comparing A vs. B and A vs. C. One hundred transcripts differentially expressed in group A vs. B have been shown to be regulated by P, suggesting compromised P signaling in the endometrium. The P receptor (PR) mutation PROGINS was not detected in women from group A. Semi-quantitation of immunoreactive PRA/B, PRB and Sp1 (a transcription factor related to P signaling) in paraffin-embedded endometrial sections, did not show statistically significant differences amongst groups. However immunostaining glycodelin was significantly decreased in endometrial samples from group A. Conclusions: We conclude that some cases of repeated implantation failure could be associated with an aberrant gene expression profile. Compromised P signaling might be the underlying mechanism for such endometrial gene expression deregulation in women with repeated implantation failure.

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    KW - Gene expression

    KW - Implantation failure

    KW - PROGINS

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    DO - 10.1186/1477-7827-12-92

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    VL - 12

    JO - Reproductive Biology and Endocrinology

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