Embryotoxicity of serum from women smoking cocaine base paste (CBP)

Luis J. Del Valle, Ricardo Pella, Azucena Mercedes, Luis A. Velasquez, Pedro A. Orihuela

Resultado de la investigación: Article

Resumen

Objective: Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years. Study design: Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48 h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo. Results: Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio. Conclusion: These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.

Idioma originalEnglish
Páginas (desde-hasta)28-31
Número de páginas4
PublicaciónEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volumen139
N.º1
DOI
EstadoPublished - 1 jul 2008

Huella dactilar

Ointments
Cocaine
Embryonic Structures
Smoking
Serum
Morula
Biological Assay

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Citar esto

Del Valle, Luis J. ; Pella, Ricardo ; Mercedes, Azucena ; Velasquez, Luis A. ; Orihuela, Pedro A. / Embryotoxicity of serum from women smoking cocaine base paste (CBP). En: European Journal of Obstetrics Gynecology and Reproductive Biology. 2008 ; Vol. 139, N.º 1. pp. 28-31.
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abstract = "Objective: Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years. Study design: Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48 h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo. Results: Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio. Conclusion: These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.",
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Embryotoxicity of serum from women smoking cocaine base paste (CBP). / Del Valle, Luis J.; Pella, Ricardo; Mercedes, Azucena; Velasquez, Luis A.; Orihuela, Pedro A.

En: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 139, N.º 1, 01.07.2008, p. 28-31.

Resultado de la investigación: Article

TY - JOUR

T1 - Embryotoxicity of serum from women smoking cocaine base paste (CBP)

AU - Del Valle, Luis J.

AU - Pella, Ricardo

AU - Mercedes, Azucena

AU - Velasquez, Luis A.

AU - Orihuela, Pedro A.

PY - 2008/7/1

Y1 - 2008/7/1

N2 - Objective: Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years. Study design: Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48 h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo. Results: Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio. Conclusion: These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.

AB - Objective: Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years. Study design: Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48 h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo. Results: Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio. Conclusion: These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.

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KW - Embryotoxic factors

KW - Preimplantation development

KW - Reproductive risks

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