Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes

Renato Chávez, Francisco Fierro, Felipe Gordillo, Juan Francisco Martín, Jaime Eyzaguirre

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

22 Citas (Scopus)

Resumen

The electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum has been resolved. Using contour-clamped homogeneous electric field gel electrophoresis, five chromosomal bands were separated, with estimated sizes of 7.1, 5.2, 3.7, 2.9 and 2.3 Mbp, giving a total genome size of 21.2 Mbp. To our knowledge, this is the smallest Penicillium genome determined so far. By Southern blots and using homologous probes, the chromosomal location of five xylanolytic genes from P. purpurogenum was determined: axeI (acetyl xylan esterase I), xynB (endoxylanase B) and abf1 (arabinofuranosidase 1) in chromosome I, xynA (endoxylanase A) in chromosome II, and axeII (acetyl xylan esterase II) in chromosome III. This is the first study where the location of xylanase genes in a Penicillium genome has been established.

Idioma originalInglés
Páginas (desde-hasta)379-383
Número de páginas5
PublicaciónFEMS Microbiology Letters
Volumen205
N.º2
DOI
EstadoPublicada - 18 dic. 2001

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular
  • Genética

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