Efficient lung recruitment of respiratory syncytial virus-specific Th1 cells induced by recombinant bacillus Calmette-Guérin promotes virus clearance and protects from Infection

Kelly M. Cautivo, Susan M. Bueno, Claudia M. Cortes, Aniela Wozniak, Claudia A. Riedel, Alexis M. Kalergis

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

62 Citas (Scopus)

Resumen

Infection by the respiratory syncytial virus (RSV) can cause extensive inflammation and lung damage in susceptible hosts due to a Th2-biased immune response. Such a deleterious inflammatory response can be enhanced by immunization with formalin-or UV-inactivated RSV, as well as with vaccinia virus expressing the RSV-G protein. Recently, we have shown that vaccination with rBCG-expressing RSVAgs can prevent the disease in the mouse. To further understand the immunological mechanisms responsible for protection against RSV, we have characterized the T cell populations contributing to virus clearance in mice immunized with this BCG-based vaccine. We found that both CD4+ and CD8+ T cells were recruited significantly earlier to the lungs of infected mice that were previously vaccinated. Furthermore, we observed that simultaneous adoptive transfer of CD8+ and CD4+ RSV-specific T cells from vaccinated mice was required to confer protection against virus infection in naive recipients. In addition, CD4+ T cells induced by vaccination released IFN-γ after RSV challenge, indicating that protection is mediated by a Th1 immune response. These data suggest that vaccination with rBCG-expressing RSV Ags can induce a specific effector/memory Th1 immune response consisting on CD4+ and CD8 + T cells, both necessary for a fully protective response against RSV. These results support the notion that an effective induction of Th1 T cell immunity against RSV during childhood could counteract the unbalanced Th2-like immune response triggered by the natural RSV infection.

Idioma originalInglés
Páginas (desde-hasta)7633-7645
Número de páginas13
PublicaciónJournal of Immunology
Volumen185
N.º12
DOI
EstadoPublicada - 15 dic. 2010

Áreas temáticas de ASJC Scopus

  • Inmulogía y alergología
  • Inmunología

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