Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile

Klaudia L. Hernández, Renato A. Quiñones, Giovanni Daneri, E. Walter Helbling

Resultado de la investigación: Article

18 Citas (Scopus)

Resumen

The influence of solar radiation on bacterial secondary production (BSP), bacterial growth efficiency (BGE), and growth rates (μ) in upwelling zones of the Humboldt Current System (HCS) is not yet understood. Here we assessed the impact of solar radiation, with special focus on ultraviolet radiation (UVR, 280 to 400 nm) on 2 natural bacterioplankton assemblages with different light history coming from surface waters (0.5 m) and subsurface waters (80 m) off central-southern Chile (36°S). The samples were incubated under ambient irradiance for 4 to 11 h and exposed to 3 spectral radiation treatments: photosynthetically active radiation (PAR, 400 to 700 nm), PAR+UV-A (320 to 700 nm), and PAR+UVR (280, to 700 nm). BSP was estimated by 14C-leucine (protein synthesis) and [methyl-3H]-thymidine (DNA synthesis) uptake. Both bacterioplankton assemblages showed significant (p < 0.05) inhibition of BSP, BGE, and μ rates when exposed to PAR radiation; in contrast, responses to UV radiation were more variable. At noon, BSP inhibition was 49 to 53 % (PAR), 13 to 30 % (UV-A), and 5 to 14 % (UV-B) for both assemblages. At sunset, in surface assemblages, protein and DNA synthesis were more limited by UV-A than by UV-B, whereas protein synthesis in the subsurface assemblage was more inhibited by UV-B than by UV-A. The same inhibition patterns were found for BGE and μ, especially with regard to protein synthesis. The daily inhibition of BSP, BGE, and μ in both assemblages was mainly a function of PAR followed by UV-A and UV-B. Our results suggest that solar radiation could play an important role in modulating bacterioplankton dynamics (especially protein synthesis) during active upwelling periods in the HCS.

Idioma originalEnglish
Páginas (desde-hasta)19-31
Número de páginas13
PublicaciónMarine Ecology Progress Series
Volumen315
DOI
EstadoPublished - 13 jun 2006

Huella dactilar

bacterioplankton
photosynthetically active radiation
ultraviolet radiation
Chile
solar radiation
secondary production
upwelling
production technology
microbial growth
protein synthesis
protein
synthesis
DNA
surface proteins
thymidine
leucine
effect
surface water
irradiance
uptake mechanisms

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology
  • Ecology, Evolution, Behavior and Systematics

Citar esto

Hernández, Klaudia L. ; Quiñones, Renato A. ; Daneri, Giovanni ; Helbling, E. Walter. / Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile. En: Marine Ecology Progress Series. 2006 ; Vol. 315. pp. 19-31.
@article{c0d142f1e533430fa29b13ab89d303cb,
title = "Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile",
abstract = "The influence of solar radiation on bacterial secondary production (BSP), bacterial growth efficiency (BGE), and growth rates (μ) in upwelling zones of the Humboldt Current System (HCS) is not yet understood. Here we assessed the impact of solar radiation, with special focus on ultraviolet radiation (UVR, 280 to 400 nm) on 2 natural bacterioplankton assemblages with different light history coming from surface waters (0.5 m) and subsurface waters (80 m) off central-southern Chile (36°S). The samples were incubated under ambient irradiance for 4 to 11 h and exposed to 3 spectral radiation treatments: photosynthetically active radiation (PAR, 400 to 700 nm), PAR+UV-A (320 to 700 nm), and PAR+UVR (280, to 700 nm). BSP was estimated by 14C-leucine (protein synthesis) and [methyl-3H]-thymidine (DNA synthesis) uptake. Both bacterioplankton assemblages showed significant (p < 0.05) inhibition of BSP, BGE, and μ rates when exposed to PAR radiation; in contrast, responses to UV radiation were more variable. At noon, BSP inhibition was 49 to 53 {\%} (PAR), 13 to 30 {\%} (UV-A), and 5 to 14 {\%} (UV-B) for both assemblages. At sunset, in surface assemblages, protein and DNA synthesis were more limited by UV-A than by UV-B, whereas protein synthesis in the subsurface assemblage was more inhibited by UV-B than by UV-A. The same inhibition patterns were found for BGE and μ, especially with regard to protein synthesis. The daily inhibition of BSP, BGE, and μ in both assemblages was mainly a function of PAR followed by UV-A and UV-B. Our results suggest that solar radiation could play an important role in modulating bacterioplankton dynamics (especially protein synthesis) during active upwelling periods in the HCS.",
keywords = "Bacterioplankton, Growth rates, Humboldt Current System, Par effects, Upwelling, UV effects",
author = "Hern{\'a}ndez, {Klaudia L.} and Qui{\~n}ones, {Renato A.} and Giovanni Daneri and Helbling, {E. Walter}",
year = "2006",
month = "6",
day = "13",
doi = "10.3354/meps315019",
language = "English",
volume = "315",
pages = "19--31",
journal = "Marine Ecology - Progress Series",
issn = "0171-8630",
publisher = "Inter-Research",

}

Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile. / Hernández, Klaudia L.; Quiñones, Renato A.; Daneri, Giovanni; Helbling, E. Walter.

En: Marine Ecology Progress Series, Vol. 315, 13.06.2006, p. 19-31.

Resultado de la investigación: Article

TY - JOUR

T1 - Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile

AU - Hernández, Klaudia L.

AU - Quiñones, Renato A.

AU - Daneri, Giovanni

AU - Helbling, E. Walter

PY - 2006/6/13

Y1 - 2006/6/13

N2 - The influence of solar radiation on bacterial secondary production (BSP), bacterial growth efficiency (BGE), and growth rates (μ) in upwelling zones of the Humboldt Current System (HCS) is not yet understood. Here we assessed the impact of solar radiation, with special focus on ultraviolet radiation (UVR, 280 to 400 nm) on 2 natural bacterioplankton assemblages with different light history coming from surface waters (0.5 m) and subsurface waters (80 m) off central-southern Chile (36°S). The samples were incubated under ambient irradiance for 4 to 11 h and exposed to 3 spectral radiation treatments: photosynthetically active radiation (PAR, 400 to 700 nm), PAR+UV-A (320 to 700 nm), and PAR+UVR (280, to 700 nm). BSP was estimated by 14C-leucine (protein synthesis) and [methyl-3H]-thymidine (DNA synthesis) uptake. Both bacterioplankton assemblages showed significant (p < 0.05) inhibition of BSP, BGE, and μ rates when exposed to PAR radiation; in contrast, responses to UV radiation were more variable. At noon, BSP inhibition was 49 to 53 % (PAR), 13 to 30 % (UV-A), and 5 to 14 % (UV-B) for both assemblages. At sunset, in surface assemblages, protein and DNA synthesis were more limited by UV-A than by UV-B, whereas protein synthesis in the subsurface assemblage was more inhibited by UV-B than by UV-A. The same inhibition patterns were found for BGE and μ, especially with regard to protein synthesis. The daily inhibition of BSP, BGE, and μ in both assemblages was mainly a function of PAR followed by UV-A and UV-B. Our results suggest that solar radiation could play an important role in modulating bacterioplankton dynamics (especially protein synthesis) during active upwelling periods in the HCS.

AB - The influence of solar radiation on bacterial secondary production (BSP), bacterial growth efficiency (BGE), and growth rates (μ) in upwelling zones of the Humboldt Current System (HCS) is not yet understood. Here we assessed the impact of solar radiation, with special focus on ultraviolet radiation (UVR, 280 to 400 nm) on 2 natural bacterioplankton assemblages with different light history coming from surface waters (0.5 m) and subsurface waters (80 m) off central-southern Chile (36°S). The samples were incubated under ambient irradiance for 4 to 11 h and exposed to 3 spectral radiation treatments: photosynthetically active radiation (PAR, 400 to 700 nm), PAR+UV-A (320 to 700 nm), and PAR+UVR (280, to 700 nm). BSP was estimated by 14C-leucine (protein synthesis) and [methyl-3H]-thymidine (DNA synthesis) uptake. Both bacterioplankton assemblages showed significant (p < 0.05) inhibition of BSP, BGE, and μ rates when exposed to PAR radiation; in contrast, responses to UV radiation were more variable. At noon, BSP inhibition was 49 to 53 % (PAR), 13 to 30 % (UV-A), and 5 to 14 % (UV-B) for both assemblages. At sunset, in surface assemblages, protein and DNA synthesis were more limited by UV-A than by UV-B, whereas protein synthesis in the subsurface assemblage was more inhibited by UV-B than by UV-A. The same inhibition patterns were found for BGE and μ, especially with regard to protein synthesis. The daily inhibition of BSP, BGE, and μ in both assemblages was mainly a function of PAR followed by UV-A and UV-B. Our results suggest that solar radiation could play an important role in modulating bacterioplankton dynamics (especially protein synthesis) during active upwelling periods in the HCS.

KW - Bacterioplankton

KW - Growth rates

KW - Humboldt Current System

KW - Par effects

KW - Upwelling

KW - UV effects

UR - http://www.scopus.com/inward/record.url?scp=33746088503&partnerID=8YFLogxK

U2 - 10.3354/meps315019

DO - 10.3354/meps315019

M3 - Article

AN - SCOPUS:33746088503

VL - 315

SP - 19

EP - 31

JO - Marine Ecology - Progress Series

JF - Marine Ecology - Progress Series

SN - 0171-8630

ER -