Effects of CDP-Choline on acetylcholine-induced relaxation of the perfused carotid vascular beds of the rat

G. Pinardi, T. Pelissier, V. Kramer, C. Paeile, H. F. Miranda

Resultado de la investigación: Article

10 Citas (Scopus)

Resumen

1. The effects of an infusion of cytidine-5′-diphosphocholine (CDP-choline) on the relaxation induced by exogenous acetylcholine (ACh) was studied in the isolated and perfuded external (ECB) and internal (ICB) carotid vascular beds of the rat. Changes in perfusion pressure were recorded during a dose-response curve to ACh and after a 30 min perfusion with CDP-choline (1 mg/min). 2. ACh induced a dose-dependent relaxation in both vascular beds, indicating the presence of muscarinic receptors. The affinity of the receptors for ACh in the ICB was significantly lower than in the ECB (ED50: 120 ± 21.4 ng and 69 ± 10.3 ng, respectively). 3. In the ICB the infusion of CDP-choline for 30 min significantly shifted the dose-response curve to ACh to the left, potentiating the relaxation. This effect was not seen in the ECB. 4. The infusion of hemicholinium (4 μM) for 30 min together with CDP-choline completely prevented the potentiation of exogenous ACh-induced relaxation in the ICB. 5. The results of the present work suggest that CDP-choline is acting by increasing choline levels in the cholinergic nerve terminals of the ICB, increasing the synthesis and/or release of ACh.

Idioma originalEnglish
Páginas (desde-hasta)635-638
Número de páginas4
PublicaciónGeneral Pharmacology
Volumen25
N.º4
DOI
EstadoPublished - 1 ene 1994

Huella dactilar

Cytidine Diphosphate Choline
Acetylcholine
Blood Vessels
Perfusion
Hemicholinium 3
Cholinergic Receptors
Muscarinic Receptors
Choline
Cholinergic Agents
Pressure

ASJC Scopus subject areas

  • Pharmacology

Citar esto

Pinardi, G. ; Pelissier, T. ; Kramer, V. ; Paeile, C. ; Miranda, H. F. / Effects of CDP-Choline on acetylcholine-induced relaxation of the perfused carotid vascular beds of the rat. En: General Pharmacology. 1994 ; Vol. 25, N.º 4. pp. 635-638.
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Effects of CDP-Choline on acetylcholine-induced relaxation of the perfused carotid vascular beds of the rat. / Pinardi, G.; Pelissier, T.; Kramer, V.; Paeile, C.; Miranda, H. F.

En: General Pharmacology, Vol. 25, N.º 4, 01.01.1994, p. 635-638.

Resultado de la investigación: Article

TY - JOUR

T1 - Effects of CDP-Choline on acetylcholine-induced relaxation of the perfused carotid vascular beds of the rat

AU - Pinardi, G.

AU - Pelissier, T.

AU - Kramer, V.

AU - Paeile, C.

AU - Miranda, H. F.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - 1. The effects of an infusion of cytidine-5′-diphosphocholine (CDP-choline) on the relaxation induced by exogenous acetylcholine (ACh) was studied in the isolated and perfuded external (ECB) and internal (ICB) carotid vascular beds of the rat. Changes in perfusion pressure were recorded during a dose-response curve to ACh and after a 30 min perfusion with CDP-choline (1 mg/min). 2. ACh induced a dose-dependent relaxation in both vascular beds, indicating the presence of muscarinic receptors. The affinity of the receptors for ACh in the ICB was significantly lower than in the ECB (ED50: 120 ± 21.4 ng and 69 ± 10.3 ng, respectively). 3. In the ICB the infusion of CDP-choline for 30 min significantly shifted the dose-response curve to ACh to the left, potentiating the relaxation. This effect was not seen in the ECB. 4. The infusion of hemicholinium (4 μM) for 30 min together with CDP-choline completely prevented the potentiation of exogenous ACh-induced relaxation in the ICB. 5. The results of the present work suggest that CDP-choline is acting by increasing choline levels in the cholinergic nerve terminals of the ICB, increasing the synthesis and/or release of ACh.

AB - 1. The effects of an infusion of cytidine-5′-diphosphocholine (CDP-choline) on the relaxation induced by exogenous acetylcholine (ACh) was studied in the isolated and perfuded external (ECB) and internal (ICB) carotid vascular beds of the rat. Changes in perfusion pressure were recorded during a dose-response curve to ACh and after a 30 min perfusion with CDP-choline (1 mg/min). 2. ACh induced a dose-dependent relaxation in both vascular beds, indicating the presence of muscarinic receptors. The affinity of the receptors for ACh in the ICB was significantly lower than in the ECB (ED50: 120 ± 21.4 ng and 69 ± 10.3 ng, respectively). 3. In the ICB the infusion of CDP-choline for 30 min significantly shifted the dose-response curve to ACh to the left, potentiating the relaxation. This effect was not seen in the ECB. 4. The infusion of hemicholinium (4 μM) for 30 min together with CDP-choline completely prevented the potentiation of exogenous ACh-induced relaxation in the ICB. 5. The results of the present work suggest that CDP-choline is acting by increasing choline levels in the cholinergic nerve terminals of the ICB, increasing the synthesis and/or release of ACh.

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KW - carotid vascular beds

KW - Cytidine-5′-diphosphocholine

KW - hemicholinium

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