Effect of temperature and photoperiod on prolactin transcription in Cyprinus carpio

Jaime Figueroa, Ariel Reyes, Mauricio Ríos, María Inés Vera, Manuel Krauskopf

Resultado de la investigación: Article

26 Citas (Scopus)

Resumen

We studied the effect of temperature and photoperiod on prolactin (PRL) gene expression in the pituitaries of summer-aclimatized carp (C. carpio). To assess the level of PRL gene transcription, we used a 24mer synthetic oligonucleotide probe derived from the known carp PRL gene sequence. Employing in situ hybridization assays, we observed high expression of PRL mRNA in the rostral pars distalis of summer-acclimatized carp in contrast to the almost negligible PRL transcription which occurs in the winter-acclimatized fish. When experimental combinations of long and short photoperiods with 10°C and 20°C environmental temperatures were studied, only a short photoperiod (8L-16D) in summer-acclimatized carp acclimated to a winter temperature (10°C) markedly depressed PRL gene expression. Our observations indicate that photoperiod constitutes a particularly relevant modulator in the neuroendocrine cascade that activates PRL transcription in the carp.

Idioma originalEnglish
Páginas (desde-hasta)353-357
Número de páginas5
PublicaciónZoological Science
Volumen14
N.º2
EstadoPublished - abr 1997

Huella dactilar

prolactin
Cyprinus carpio
transcription (genetics)
photoperiod
carp
temperature
summer
gene expression
oligonucleotide probes
winter
in situ hybridization
ambient temperature
nucleotide sequences
assays
fish
genes

ASJC Scopus subject areas

  • Animal Science and Zoology

Citar esto

Figueroa, J., Reyes, A., Ríos, M., Vera, M. I., & Krauskopf, M. (1997). Effect of temperature and photoperiod on prolactin transcription in Cyprinus carpio. Zoological Science, 14(2), 353-357.
Figueroa, Jaime ; Reyes, Ariel ; Ríos, Mauricio ; Vera, María Inés ; Krauskopf, Manuel. / Effect of temperature and photoperiod on prolactin transcription in Cyprinus carpio. En: Zoological Science. 1997 ; Vol. 14, N.º 2. pp. 353-357.
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abstract = "We studied the effect of temperature and photoperiod on prolactin (PRL) gene expression in the pituitaries of summer-aclimatized carp (C. carpio). To assess the level of PRL gene transcription, we used a 24mer synthetic oligonucleotide probe derived from the known carp PRL gene sequence. Employing in situ hybridization assays, we observed high expression of PRL mRNA in the rostral pars distalis of summer-acclimatized carp in contrast to the almost negligible PRL transcription which occurs in the winter-acclimatized fish. When experimental combinations of long and short photoperiods with 10°C and 20°C environmental temperatures were studied, only a short photoperiod (8L-16D) in summer-acclimatized carp acclimated to a winter temperature (10°C) markedly depressed PRL gene expression. Our observations indicate that photoperiod constitutes a particularly relevant modulator in the neuroendocrine cascade that activates PRL transcription in the carp.",
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Figueroa, J, Reyes, A, Ríos, M, Vera, MI & Krauskopf, M 1997, 'Effect of temperature and photoperiod on prolactin transcription in Cyprinus carpio', Zoological Science, vol. 14, n.º 2, pp. 353-357.

Effect of temperature and photoperiod on prolactin transcription in Cyprinus carpio. / Figueroa, Jaime; Reyes, Ariel; Ríos, Mauricio; Vera, María Inés; Krauskopf, Manuel.

En: Zoological Science, Vol. 14, N.º 2, 04.1997, p. 353-357.

Resultado de la investigación: Article

TY - JOUR

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AU - Reyes, Ariel

AU - Ríos, Mauricio

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AU - Krauskopf, Manuel

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