TY - JOUR
T1 - Effect of Several HIV Antigens Simultaneously Loaded with G2-NN16 Carbosilane Dendrimer in the Cell Uptake and Functionality of Human Dendritic Cells
AU - Sepúlveda-Crespo, Daniel
AU - Vacas-Córdoba, Enrique
AU - Márquez-Miranda, Valeria
AU - Araya-Durán, Ingrid
AU - Gómez, Rafael
AU - Mata, Francisco Javier De La
AU - González-Nilo, Fernando Danilo
AU - Muñoz-Fernández, Ma Ángeles
N1 - Funding Information:
This work has been partially funded by the RD12/0017/0037 project as part of the Accion Estrategica en Salud, Plan Nacional de Investigacion Cientifi ca, Desarrollo e Innovacion Tecnologica 2008-2011 and was co-financed by the Instituto de Salud Carlos III (Subdireccion General de Evaluacion) and the Fondo Europeo de Desarrollo Regional (FEDER), RETIC PT13/0010/0028, Fondo de Investigacion Sanitaria (FIS) (grant no. PI13/02016), CTQ2014-54004-P (MIMECO), Comunidad de Madrid (grant nos. S-2010/BMD-2351 and S- 2010/BMD-2332], CYTED 214RT0482. CIBER-BBN is an initiative funded by the VI National R D i Plan 2008-2011, IniciativaIngenio 2010, the Consolider Program, and CIBER Actions and is financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/12/21
Y1 - 2016/12/21
N2 - Dendrimers are highly branched, star-shaped, and nanosized polymers that have been proposed as new carriers for specific HIV-1 peptides. Dendritic cells (DCs) are the most-potent antigen-presenting cells that play a major role in the development of cell-mediated immunotherapy due to the generation and regulation of adaptive immune responses against HIV-1. This article reports on the associated behavior of two or three HIV-derived peptides simultaneously (p24/gp160 or p24/gp160/NEF) with cationic carbosilane dendrimer G2-NN16. We have found that (i) immature DCs (iDCs) and mature (mDCs) did not capture efficiently HIV peptides regarding the uptake level when cells were treated with G2-NN16-peptide complex alone; (ii) the ability of DCs to migrate was not depending on the peptides presence; and (iii) with the use of molecular dynamic simulation, a mixture of peptides decreased the cell uptake of the other peptides (in particular, NEF hinders the binding of more peptides and is especially obstructing of the binding of gp160 to G2-NN16). The results suggest that G2-NN16 cannot be considered as an alternative carrier for delivering two or more HIV-derived peptides to DCs.
AB - Dendrimers are highly branched, star-shaped, and nanosized polymers that have been proposed as new carriers for specific HIV-1 peptides. Dendritic cells (DCs) are the most-potent antigen-presenting cells that play a major role in the development of cell-mediated immunotherapy due to the generation and regulation of adaptive immune responses against HIV-1. This article reports on the associated behavior of two or three HIV-derived peptides simultaneously (p24/gp160 or p24/gp160/NEF) with cationic carbosilane dendrimer G2-NN16. We have found that (i) immature DCs (iDCs) and mature (mDCs) did not capture efficiently HIV peptides regarding the uptake level when cells were treated with G2-NN16-peptide complex alone; (ii) the ability of DCs to migrate was not depending on the peptides presence; and (iii) with the use of molecular dynamic simulation, a mixture of peptides decreased the cell uptake of the other peptides (in particular, NEF hinders the binding of more peptides and is especially obstructing of the binding of gp160 to G2-NN16). The results suggest that G2-NN16 cannot be considered as an alternative carrier for delivering two or more HIV-derived peptides to DCs.
UR - http://www.scopus.com/inward/record.url?scp=85006957132&partnerID=8YFLogxK
U2 - 10.1021/acs.bioconjchem.6b00623
DO - 10.1021/acs.bioconjchem.6b00623
M3 - Article
AN - SCOPUS:85006957132
SN - 1043-1802
VL - 27
SP - 2844
EP - 2849
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 12
ER -