Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

Rodrigo A. Mancilla, Paulo Canessa, Augusto Manubens, Rafael Vicuña

Resultado de la investigación: Article

14 Citas (Scopus)

Resumen

The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell.

Idioma originalEnglish
Páginas (desde-hasta)656-661
Número de páginas6
PublicaciónFungal Genetics and Biology
Volumen47
N.º7
DOI
EstadoPublished - 1 jul 2010

Huella dactilar

manganese peroxidase
Coriolaceae
Basidiomycota
Manganese
Enzymes
Laccase
Immunoelectron Microscopy
Mycelium
Enzyme Assays
Cycloheximide
Fungi
Western Blotting
Metals

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Citar esto

Mancilla, Rodrigo A. ; Canessa, Paulo ; Manubens, Augusto ; Vicuña, Rafael. / Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora. En: Fungal Genetics and Biology. 2010 ; Vol. 47, N.º 7. pp. 656-661.
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Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora. / Mancilla, Rodrigo A.; Canessa, Paulo; Manubens, Augusto; Vicuña, Rafael.

En: Fungal Genetics and Biology, Vol. 47, N.º 7, 01.07.2010, p. 656-661.

Resultado de la investigación: Article

TY - JOUR

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AU - Mancilla, Rodrigo A.

AU - Canessa, Paulo

AU - Manubens, Augusto

AU - Vicuña, Rafael

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AB - The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell.

KW - Ligninolysis

KW - Manganese

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