Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

Rodrigo A. Mancilla, Paulo Canessa, Augusto Manubens, Rafael Vicuña

Resultado de la investigación: Contribución a una revistaArtículo

14 Citas (Scopus)

Resumen

The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell.

Idioma originalInglés
Páginas (desde-hasta)656-661
Número de páginas6
PublicaciónFungal Genetics and Biology
Volumen47
N.º7
DOI
EstadoPublicada - 1 jul 2010

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Genética

Huella Profundice en los temas de investigación de 'Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora'. En conjunto forman una huella única.

  • Citar esto