Effect of drimenol and synthetic derivatives on growth and germination of Botrytis cinerea

Evaluation of possible mechanism of action

Christian Robles-Kelly, Julia Rubio, Mario Thomas, Claudia Sedán, Rolando Martinez, Andrés F. Olea, Héctor Carrasco, Lautaro Taborga, Evelyn Silva-Moreno

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

The aim of this study was to determine the antifungal activity of Drimenol (1) and its synthetic derivatives, nordrimenone (2), drimenyl acetate (3), and drimenyl-epoxy-acetate (4), and to establish a possible mechanism of action for drimenol. For that, the effect of each compound on mycelial growth of Botrytis cinerea was assessed. Our results showed that compounds 1, 2, 3 and 4 are able to affect Botrytis cinerea growth with EC50 values of 80, 92, 80 and 314 ppm, respectively. These values suggest that the activity of these compounds is mainly determined by presence of the double bond between carbons 7 and 8 of the drimane ring. In addition, germination of B. cinerea in presence of 40 and 80 ppm of drimenol is reduced almost to a half of the control value. Finally, in order to elucidate a possible mechanism by which drimenol is affecting B. cinerea, the determination of membrane integrity, reactive oxygen species production and gene expression studies of specific genes were performed.

Idioma originalEnglish
Páginas (desde-hasta)50-56
Número de páginas7
PublicaciónPesticide Biochemistry and Physiology
Volumen141
DOI
EstadoPublished - 1 sep 2017

Huella dactilar

Botrytis
Germination
Botrytis cinerea
mechanism of action
chemical derivatives
germination
Growth
Acetates
acetates
epoxides
reactive oxygen species
Reactive Oxygen Species
Carbon
Gene Expression
gene expression
Membranes
drimenol
carbon
Genes
genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Citar esto

Robles-Kelly, Christian ; Rubio, Julia ; Thomas, Mario ; Sedán, Claudia ; Martinez, Rolando ; Olea, Andrés F. ; Carrasco, Héctor ; Taborga, Lautaro ; Silva-Moreno, Evelyn. / Effect of drimenol and synthetic derivatives on growth and germination of Botrytis cinerea : Evaluation of possible mechanism of action. En: Pesticide Biochemistry and Physiology. 2017 ; Vol. 141. pp. 50-56.
@article{01bfec1c1ff94fbe86d890aa94499afe,
title = "Effect of drimenol and synthetic derivatives on growth and germination of Botrytis cinerea: Evaluation of possible mechanism of action",
abstract = "The aim of this study was to determine the antifungal activity of Drimenol (1) and its synthetic derivatives, nordrimenone (2), drimenyl acetate (3), and drimenyl-epoxy-acetate (4), and to establish a possible mechanism of action for drimenol. For that, the effect of each compound on mycelial growth of Botrytis cinerea was assessed. Our results showed that compounds 1, 2, 3 and 4 are able to affect Botrytis cinerea growth with EC50 values of 80, 92, 80 and 314 ppm, respectively. These values suggest that the activity of these compounds is mainly determined by presence of the double bond between carbons 7 and 8 of the drimane ring. In addition, germination of B. cinerea in presence of 40 and 80 ppm of drimenol is reduced almost to a half of the control value. Finally, in order to elucidate a possible mechanism by which drimenol is affecting B. cinerea, the determination of membrane integrity, reactive oxygen species production and gene expression studies of specific genes were performed.",
keywords = "Antifungal activity, Botrytis cinerea, Drimenol",
author = "Christian Robles-Kelly and Julia Rubio and Mario Thomas and Claudia Sed{\'a}n and Rolando Martinez and Olea, {Andr{\'e}s F.} and H{\'e}ctor Carrasco and Lautaro Taborga and Evelyn Silva-Moreno",
year = "2017",
month = "9",
day = "1",
doi = "10.1016/j.pestbp.2016.11.006",
language = "English",
volume = "141",
pages = "50--56",
journal = "Pesticide Biochemistry and Physiology",
issn = "0048-3575",
publisher = "Academic Press Inc.",

}

Effect of drimenol and synthetic derivatives on growth and germination of Botrytis cinerea : Evaluation of possible mechanism of action. / Robles-Kelly, Christian; Rubio, Julia; Thomas, Mario; Sedán, Claudia; Martinez, Rolando; Olea, Andrés F.; Carrasco, Héctor; Taborga, Lautaro; Silva-Moreno, Evelyn.

En: Pesticide Biochemistry and Physiology, Vol. 141, 01.09.2017, p. 50-56.

Resultado de la investigación: Article

TY - JOUR

T1 - Effect of drimenol and synthetic derivatives on growth and germination of Botrytis cinerea

T2 - Evaluation of possible mechanism of action

AU - Robles-Kelly, Christian

AU - Rubio, Julia

AU - Thomas, Mario

AU - Sedán, Claudia

AU - Martinez, Rolando

AU - Olea, Andrés F.

AU - Carrasco, Héctor

AU - Taborga, Lautaro

AU - Silva-Moreno, Evelyn

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The aim of this study was to determine the antifungal activity of Drimenol (1) and its synthetic derivatives, nordrimenone (2), drimenyl acetate (3), and drimenyl-epoxy-acetate (4), and to establish a possible mechanism of action for drimenol. For that, the effect of each compound on mycelial growth of Botrytis cinerea was assessed. Our results showed that compounds 1, 2, 3 and 4 are able to affect Botrytis cinerea growth with EC50 values of 80, 92, 80 and 314 ppm, respectively. These values suggest that the activity of these compounds is mainly determined by presence of the double bond between carbons 7 and 8 of the drimane ring. In addition, germination of B. cinerea in presence of 40 and 80 ppm of drimenol is reduced almost to a half of the control value. Finally, in order to elucidate a possible mechanism by which drimenol is affecting B. cinerea, the determination of membrane integrity, reactive oxygen species production and gene expression studies of specific genes were performed.

AB - The aim of this study was to determine the antifungal activity of Drimenol (1) and its synthetic derivatives, nordrimenone (2), drimenyl acetate (3), and drimenyl-epoxy-acetate (4), and to establish a possible mechanism of action for drimenol. For that, the effect of each compound on mycelial growth of Botrytis cinerea was assessed. Our results showed that compounds 1, 2, 3 and 4 are able to affect Botrytis cinerea growth with EC50 values of 80, 92, 80 and 314 ppm, respectively. These values suggest that the activity of these compounds is mainly determined by presence of the double bond between carbons 7 and 8 of the drimane ring. In addition, germination of B. cinerea in presence of 40 and 80 ppm of drimenol is reduced almost to a half of the control value. Finally, in order to elucidate a possible mechanism by which drimenol is affecting B. cinerea, the determination of membrane integrity, reactive oxygen species production and gene expression studies of specific genes were performed.

KW - Antifungal activity

KW - Botrytis cinerea

KW - Drimenol

UR - http://www.scopus.com/inward/record.url?scp=85007013007&partnerID=8YFLogxK

U2 - 10.1016/j.pestbp.2016.11.006

DO - 10.1016/j.pestbp.2016.11.006

M3 - Article

VL - 141

SP - 50

EP - 56

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

SN - 0048-3575

ER -