Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells

R. Alvarez, O. Inostroza, M. Garavaglia, N. P. Minton, D. Paredes-Sabja, F. Gil

Resultado de la investigación: Contribución a una revistaArtículo

1 Cita (Scopus)

Resumen

Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persisterlike non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10× the MIC of every antibiotic for up to 6 days. Results: Using up to 10× the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.

Idioma originalInglés
Páginas (desde-hasta)2396-2399
Número de páginas4
PublicaciónJournal of Antimicrobial Chemotherapy
Volumen73
N.º9
DOI
EstadoPublicada - 1 ene 2018

Áreas temáticas de ASJC Scopus

  • Enfermedades infecciosas
  • Farmacología
  • Microbiología (médica)
  • Farmacología (médica)

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