Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells

R. Alvarez, O. Inostroza, M. Garavaglia, N. P. Minton, D. Paredes-Sabja, F. Gil

Resultado de la investigación: Article

Resumen

Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persisterlike non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10× the MIC of every antibiotic for up to 6 days. Results: Using up to 10× the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.

Idioma originalEnglish
Páginas (desde-hasta)2396-2399
Número de páginas4
PublicaciónJournal of Antimicrobial Chemotherapy
Volumen73
N.º9
DOI
EstadoPublished - 1 ene 2018

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)

Citar esto

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title = "Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells",
abstract = "Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persisterlike non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10× the MIC of every antibiotic for up to 6 days. Results: Using up to 10× the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.",
author = "R. Alvarez and O. Inostroza and M. Garavaglia and Minton, {N. P.} and D. Paredes-Sabja and F. Gil",
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Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells. / Alvarez, R.; Inostroza, O.; Garavaglia, M.; Minton, N. P.; Paredes-Sabja, D.; Gil, F.

En: Journal of Antimicrobial Chemotherapy, Vol. 73, N.º 9, 01.01.2018, p. 2396-2399.

Resultado de la investigación: Article

TY - JOUR

T1 - Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells

AU - Alvarez, R.

AU - Inostroza, O.

AU - Garavaglia, M.

AU - Minton, N. P.

AU - Paredes-Sabja, D.

AU - Gil, F.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persisterlike non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10× the MIC of every antibiotic for up to 6 days. Results: Using up to 10× the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.

AB - Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persisterlike non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10× the MIC of every antibiotic for up to 6 days. Results: Using up to 10× the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen.

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U2 - 10.1093/jac/dky186

DO - 10.1093/jac/dky186

M3 - Article

VL - 73

SP - 2396

EP - 2399

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

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