Edible scaffolds based on non-mammalian biopolymers for myoblast growth

Javier Enrione, Jonny J. Blaker, Donald I. Brown, Caroline R. Weinstein-Oppenheimer, Marzena Pepczynska, Yusser Olguín, Elizabeth Sánchez, Cristian A. Acevedo

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

In vitro meat has recently emerged as a new concept in food biotechnology. Methods to produce in vitro meat generally involve the growth of muscle cells that are cultured on scaffolds using bioreactors. Suitable scaffold design and manufacture are critical to downstream culture and meat production. Most current scaffolds are based on mammalian-derived biomaterials, the use of which is counter to the desire to obviate mammal slaughter in artificial meat production. Consequently, most of the knowledge is related to the design and control of scaffold properties based on these mammalian-sourced materials. To address this, four different scaffold materials were formulated using non-mammalian sources, namely, salmon gelatin, alginate, and additives including gelling agents and plasticizers. The scaffolds were produced using a freeze-drying process, and the physical, mechanical, and biological properties of the scaffolds were evaluated. The most promising scaffolds were produced from salmon gelatin, alginate, agarose, and glycerol, which exhibited relatively large pore sizes (~200 μmdiameter) and biocompatibility, permitting myoblast cell adhesion (~40%) and growth (~24 h duplication time). The biodegradation profiles of the scaffolds were followed, and were observed to be less than 25% after 4 weeks. The scaffolds enabled suitable myogenic response, with high cell proliferation, viability, and adequate cell distribution throughout. This system composed of non-mammalian edible scaffold material and muscle-cells is promising for the production of in vitro meat.

Idioma originalEnglish
Número de artículo1404
PublicaciónMaterials
Volumen10
N.º12
DOI
EstadoPublished - 8 dic 2017

Huella dactilar

Biopolymers
Scaffolds
Meats
Alginate
Gelatin
Muscle
Plasticizers
Mammals
Cell adhesion
Cell proliferation
Cell growth
Biocompatible Materials
Scaffolds (biology)
Biotechnology
Biodegradation
Bioreactors
Glycerol
Biocompatibility
Biomaterials
Sepharose

ASJC Scopus subject areas

  • Materials Science(all)

Citar esto

Enrione, J., Blaker, J. J., Brown, D. I., Weinstein-Oppenheimer, C. R., Pepczynska, M., Olguín, Y., ... Acevedo, C. A. (2017). Edible scaffolds based on non-mammalian biopolymers for myoblast growth. Materials, 10(12), [1404]. https://doi.org/10.3390/ma10121404
Enrione, Javier ; Blaker, Jonny J. ; Brown, Donald I. ; Weinstein-Oppenheimer, Caroline R. ; Pepczynska, Marzena ; Olguín, Yusser ; Sánchez, Elizabeth ; Acevedo, Cristian A. / Edible scaffolds based on non-mammalian biopolymers for myoblast growth. En: Materials. 2017 ; Vol. 10, N.º 12.
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abstract = "In vitro meat has recently emerged as a new concept in food biotechnology. Methods to produce in vitro meat generally involve the growth of muscle cells that are cultured on scaffolds using bioreactors. Suitable scaffold design and manufacture are critical to downstream culture and meat production. Most current scaffolds are based on mammalian-derived biomaterials, the use of which is counter to the desire to obviate mammal slaughter in artificial meat production. Consequently, most of the knowledge is related to the design and control of scaffold properties based on these mammalian-sourced materials. To address this, four different scaffold materials were formulated using non-mammalian sources, namely, salmon gelatin, alginate, and additives including gelling agents and plasticizers. The scaffolds were produced using a freeze-drying process, and the physical, mechanical, and biological properties of the scaffolds were evaluated. The most promising scaffolds were produced from salmon gelatin, alginate, agarose, and glycerol, which exhibited relatively large pore sizes (~200 μmdiameter) and biocompatibility, permitting myoblast cell adhesion (~40{\%}) and growth (~24 h duplication time). The biodegradation profiles of the scaffolds were followed, and were observed to be less than 25{\%} after 4 weeks. The scaffolds enabled suitable myogenic response, with high cell proliferation, viability, and adequate cell distribution throughout. This system composed of non-mammalian edible scaffold material and muscle-cells is promising for the production of in vitro meat.",
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Enrione, J, Blaker, JJ, Brown, DI, Weinstein-Oppenheimer, CR, Pepczynska, M, Olguín, Y, Sánchez, E & Acevedo, CA 2017, 'Edible scaffolds based on non-mammalian biopolymers for myoblast growth', Materials, vol. 10, n.º 12, 1404. https://doi.org/10.3390/ma10121404

Edible scaffolds based on non-mammalian biopolymers for myoblast growth. / Enrione, Javier; Blaker, Jonny J.; Brown, Donald I.; Weinstein-Oppenheimer, Caroline R.; Pepczynska, Marzena; Olguín, Yusser; Sánchez, Elizabeth; Acevedo, Cristian A.

En: Materials, Vol. 10, N.º 12, 1404, 08.12.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Edible scaffolds based on non-mammalian biopolymers for myoblast growth

AU - Enrione, Javier

AU - Blaker, Jonny J.

AU - Brown, Donald I.

AU - Weinstein-Oppenheimer, Caroline R.

AU - Pepczynska, Marzena

AU - Olguín, Yusser

AU - Sánchez, Elizabeth

AU - Acevedo, Cristian A.

PY - 2017/12/8

Y1 - 2017/12/8

N2 - In vitro meat has recently emerged as a new concept in food biotechnology. Methods to produce in vitro meat generally involve the growth of muscle cells that are cultured on scaffolds using bioreactors. Suitable scaffold design and manufacture are critical to downstream culture and meat production. Most current scaffolds are based on mammalian-derived biomaterials, the use of which is counter to the desire to obviate mammal slaughter in artificial meat production. Consequently, most of the knowledge is related to the design and control of scaffold properties based on these mammalian-sourced materials. To address this, four different scaffold materials were formulated using non-mammalian sources, namely, salmon gelatin, alginate, and additives including gelling agents and plasticizers. The scaffolds were produced using a freeze-drying process, and the physical, mechanical, and biological properties of the scaffolds were evaluated. The most promising scaffolds were produced from salmon gelatin, alginate, agarose, and glycerol, which exhibited relatively large pore sizes (~200 μmdiameter) and biocompatibility, permitting myoblast cell adhesion (~40%) and growth (~24 h duplication time). The biodegradation profiles of the scaffolds were followed, and were observed to be less than 25% after 4 weeks. The scaffolds enabled suitable myogenic response, with high cell proliferation, viability, and adequate cell distribution throughout. This system composed of non-mammalian edible scaffold material and muscle-cells is promising for the production of in vitro meat.

AB - In vitro meat has recently emerged as a new concept in food biotechnology. Methods to produce in vitro meat generally involve the growth of muscle cells that are cultured on scaffolds using bioreactors. Suitable scaffold design and manufacture are critical to downstream culture and meat production. Most current scaffolds are based on mammalian-derived biomaterials, the use of which is counter to the desire to obviate mammal slaughter in artificial meat production. Consequently, most of the knowledge is related to the design and control of scaffold properties based on these mammalian-sourced materials. To address this, four different scaffold materials were formulated using non-mammalian sources, namely, salmon gelatin, alginate, and additives including gelling agents and plasticizers. The scaffolds were produced using a freeze-drying process, and the physical, mechanical, and biological properties of the scaffolds were evaluated. The most promising scaffolds were produced from salmon gelatin, alginate, agarose, and glycerol, which exhibited relatively large pore sizes (~200 μmdiameter) and biocompatibility, permitting myoblast cell adhesion (~40%) and growth (~24 h duplication time). The biodegradation profiles of the scaffolds were followed, and were observed to be less than 25% after 4 weeks. The scaffolds enabled suitable myogenic response, with high cell proliferation, viability, and adequate cell distribution throughout. This system composed of non-mammalian edible scaffold material and muscle-cells is promising for the production of in vitro meat.

KW - Biopolymer

KW - Edible material

KW - In vitro meat

KW - Scaffold

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U2 - 10.3390/ma10121404

DO - 10.3390/ma10121404

M3 - Article

AN - SCOPUS:85037534986

VL - 10

JO - Materials

JF - Materials

SN - 1996-1944

IS - 12

M1 - 1404

ER -

Enrione J, Blaker JJ, Brown DI, Weinstein-Oppenheimer CR, Pepczynska M, Olguín Y y otros. Edible scaffolds based on non-mammalian biopolymers for myoblast growth. Materials. 2017 dic 8;10(12). 1404. https://doi.org/10.3390/ma10121404