TY - JOUR
T1 - Dynamic expression pattern of the growth hormone receptor during early development of the Chilean flounder
AU - Fuentes, Eduardo
AU - Poblete, Erika
AU - Reyes, Ariel E.
AU - Vera, María Inés
AU - Álvarez, Marco
AU - Molina, Alfredo
N1 - Funding Information:
This work was supported by Grants No. 1050272 from FONDECYT and 03-05/I from UNAB Research Fund to A.M and FONDECYT No. 1060441 to A.E.R. We would like to thank Dr. Manuel Krauskopf for critical reading of the manuscript. We thank Juan Manuel Estrada for technical assistance and animal care in the Centro de Investigación Marina de Quintay (CIMARQ).
PY - 2008/5
Y1 - 2008/5
N2 - The entire cDNA sequence of the growth hormone receptor (GHR) of the Chilean flounder (Paralichthys. adspersus) was cloned by RT-PCR and RNA ligase rapid amplification of 5′ and 3′ends. The deduced amino acid sequence contains 641 residues and codes for the GHR1 form. The receptor includes all the structural domains and motifs responsible for its interaction with the growth hormone and growth signal transduction. Sequence comparison revealed 95 and 88% identity with other flat fish such as the Japanese flounder and Atlantic halibut respectively, but decreased to 41% with the GHR of other teleosts such as salmon. In addition we performed a phylogenetic analysis of this receptor in teleosts. RT-PCR experiments were performed to study the expression of GHR1 mRNA in different tissues of juvenile fish, detecting the transcripts in all tissues investigated with higher expressions in the liver, brain and gonads. Additionally, using whole-mount in situ hybridization in larvae stages, we observed an on and off GHR1 mRNA expression pattern. This novel finding evidences that during early development of a teleost, GHR1 is transiently expressed in somites, a source of muscle, bone and spinal chord precursors cells, suggesting a relevant role of GH in fish development. GHR1 was also temporally detected in the notochord, intestines, brain and retinal layers, before its ubiquitous establishment.
AB - The entire cDNA sequence of the growth hormone receptor (GHR) of the Chilean flounder (Paralichthys. adspersus) was cloned by RT-PCR and RNA ligase rapid amplification of 5′ and 3′ends. The deduced amino acid sequence contains 641 residues and codes for the GHR1 form. The receptor includes all the structural domains and motifs responsible for its interaction with the growth hormone and growth signal transduction. Sequence comparison revealed 95 and 88% identity with other flat fish such as the Japanese flounder and Atlantic halibut respectively, but decreased to 41% with the GHR of other teleosts such as salmon. In addition we performed a phylogenetic analysis of this receptor in teleosts. RT-PCR experiments were performed to study the expression of GHR1 mRNA in different tissues of juvenile fish, detecting the transcripts in all tissues investigated with higher expressions in the liver, brain and gonads. Additionally, using whole-mount in situ hybridization in larvae stages, we observed an on and off GHR1 mRNA expression pattern. This novel finding evidences that during early development of a teleost, GHR1 is transiently expressed in somites, a source of muscle, bone and spinal chord precursors cells, suggesting a relevant role of GH in fish development. GHR1 was also temporally detected in the notochord, intestines, brain and retinal layers, before its ubiquitous establishment.
KW - Chilean flounder
KW - Early development expression pattern
KW - Growth hormone receptor
KW - Whole mount in situ hybridization
UR - http://www.scopus.com/inward/record.url?scp=41749121700&partnerID=8YFLogxK
U2 - 10.1016/j.cbpb.2008.01.009
DO - 10.1016/j.cbpb.2008.01.009
M3 - Article
C2 - 18313961
AN - SCOPUS:41749121700
SN - 1096-4959
VL - 150
SP - 93
EP - 102
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 1
ER -