Dual transcriptomic profiling of host and microbiota during health and disease in pediatric asthma

Marcos Pérez-Losada, Eduardo Castro-Nallar, Matthew L. Bendall, Robert J. Freishtat, Keith A. Crandall

Resultado de la investigación: Article

40 Citas (Scopus)

Resumen

Background: High-throughput sequencing (HTS) analysis of microbial communities from the respiratory airways has heavily relied on the 16S rRNA gene. Given the intrinsic limitations of this approach, airway microbiome research has focused on assessing bacterial composition during health and disease, and its variation in relation to clinical and environmental factors, or other microbiomes. Consequently, very little effort has been dedicated to describing the functional characteristics of the airway microbiota and even less to explore the microbe-host interactions. Here we present a simultaneous assessment of microbiome and host functional diversity and host-microbe interactions from the same RNA-seq experiment, while accounting for variation in clinical metadata. Methods: Transcriptomic (host) and metatranscriptomic (microbiota) sequences from the nasal epithelium of 8 asthmatics and 6 healthy controls were separated in silico and mapped to available human and NCBI-NR protein reference databases. Human genes differentially expressed in asthmatics and controls were then used to infer upstream regulators involved in immune and inflammatory responses. Concomitantly, microbial genes were mapped to metabolic databases (COG, SEED, and KEGG) to infer microbial functions differentially expressed in asthmatics and controls. Finally, multivariate analysis was applied to find associations between microbiome characteristics and host upstream regulators while accounting for clinical variation. Results and Discussion: Our study showed significant differences in the metabolism of microbiomes from asthmatic and non-asthmatic children for up to 25% of the functional properties tested. Enrichment analysis of 499 differentially expressed host genes for inflammatory and immune responses revealed 43 upstream regulators differentially activated in asthma. Microbial adhesion (virulence) and Proteobacteria abundance were significantly associated with variation in the expression of the upstream regulator IL1A; suggesting that microbiome characteristics modulate host inflammatory and immune systems during asthma.

Idioma originalEnglish
Número de artículoe0131819
PublicaciónPLoS ONE
Volumen10
N.º6
DOI
EstadoPublished - 30 jun 2015

Huella dactilar

Pediatrics
Microbiota
asthma
transcriptomics
Asthma
Genes
Health
Immune system
Metadata
Metabolism
functional properties
Microbial Genes
Adhesion
genes
inflammation
Throughput
immune response
RNA
microorganisms
Proteobacteria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Pérez-Losada, Marcos ; Castro-Nallar, Eduardo ; Bendall, Matthew L. ; Freishtat, Robert J. ; Crandall, Keith A. / Dual transcriptomic profiling of host and microbiota during health and disease in pediatric asthma. En: PLoS ONE. 2015 ; Vol. 10, N.º 6.
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Dual transcriptomic profiling of host and microbiota during health and disease in pediatric asthma. / Pérez-Losada, Marcos; Castro-Nallar, Eduardo; Bendall, Matthew L.; Freishtat, Robert J.; Crandall, Keith A.

En: PLoS ONE, Vol. 10, N.º 6, e0131819, 30.06.2015.

Resultado de la investigación: Article

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AU - Pérez-Losada, Marcos

AU - Castro-Nallar, Eduardo

AU - Bendall, Matthew L.

AU - Freishtat, Robert J.

AU - Crandall, Keith A.

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