Down-regulation of the antisense mitochondrial non-coding RNAs (ncRNAs) is a unique vulnerability of cancer cells and a potential target for cancer therapy

Soledad Vidaurre, Christopher Fitzpatrick, Verónica A. Burzio, Macarena Briones, Claudio Villota, Jaime Villegas, Javiera Echenique, Luciana Oliveira-Cruz, Mariela Araya, Vincenzo Borgna, Teresa Socías, Constanza Lopez, Rodolfo Avila, Luis O. Burzio

Resultado de la investigación: Contribución a una revistaArtículo

39 Citas (Scopus)

Resumen

Hallmarks of cancer are fundamental principles involved in cancer progression. We propose an additional generalized hallmark of malignant transformation corresponding to the differential expression of a family of mitochondrial ncRNAs (ncmtRNAs) that comprises sense and antisense members, all of which contain stem-loop structures. Normal proliferating cells express sense (SncmtRNA) and antisense (ASncmtRNA) transcripts. In contrast, the ASncmtRNAs are down-regulated in tumor cells regardless of tissue of origin. Here we show that knockdown of the low copy number of the ASncmtRNAs in several tumor cell lines induces cell death by apoptosis without affecting the viability of normal cells. In addition, knockdown of ASncmtRNAs potentiates apoptotic cell death by inhibiting survivin expression, a member of the inhibitor of apoptosis (IAP) family. Down-regulation of survivin is at the translational level and is probably mediated by microRNAs generated by dicing of the double-stranded stem of the ASncmtRNAs, as suggested by evidence presented here, in which the ASncmtRNAs are bound to Dicer and knockdown of the ASncmtRNAs reduces reporter luciferase activity in a vector carrying the 3′-UTR of survivin mRNA. Taken together, down-regulation of the ASncmtRNAs constitutes a vulnerability or Achilles' heel of cancer cells, suggesting that the ASncmtRNAs are promising targets for cancer therapy.

Idioma originalInglés
Páginas (desde-hasta)27182-27198
Número de páginas17
PublicaciónJournal of Biological Chemistry
Volumen289
N.º39
DOI
EstadoPublicada - 26 sep 2014

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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    Vidaurre, S., Fitzpatrick, C., Burzio, V. A., Briones, M., Villota, C., Villegas, J., Echenique, J., Oliveira-Cruz, L., Araya, M., Borgna, V., Socías, T., Lopez, C., Avila, R., & Burzio, L. O. (2014). Down-regulation of the antisense mitochondrial non-coding RNAs (ncRNAs) is a unique vulnerability of cancer cells and a potential target for cancer therapy. Journal of Biological Chemistry, 289(39), 27182-27198. https://doi.org/10.1074/jbc.M114.558841