Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins

C. Das, G. P. Talwar, S. Ramakrishnan, M. Salahuddin, S. Kumar, V. Hingorani, E. Coutinho, H. Croxatto, E. Hemmingson, E. Johansson, T. Luukkainen, S. Shahani, K. Sundaram, H. Nash, S. J. Segal

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Serum from monkeys and human subjects immunized with an anti-hCG vaccine, Pr-β-hCG-TT, have been analyzed for their capacity to neutralize hCG/LH induced biological effects in two test systems. The monkey antiserum blocked completely the hCG induced ovulation in mice, but the same amount of the antiserum and even two-fold higher concentrations, did not reduce the number of ovulating animals on priming with ovine LH. The immunized monkey and the human sera were competent to neutralise the hCG induced testosterone production in Leydig cells. The monkey and seven out of twelve human sera did not interfere in the hLH action on these cells. Five human sera, however, showed varying degree of inhibition of hLH induced steroidogenesis in this sensitive bioassay. These subjects nevertheless maintained regular menstrual cycles and serum progesterone levels in the luteal phase were consonant with ovulation. In a subject whose serum had shown a fairly high degree of cross-reaction with hLH in Leydig cell bioassay, estradial, hLH and progesterone levels were determined on different days throughout a menstrual cycle. The hormonal profiles were normal.

Idioma originalEnglish
Páginas (desde-hasta)35-50
Número de páginas16
PublicaciónContraception
Volumen18
N.º1
DOI
EstadoPublished - 1 ene 1978

Huella dactilar

Pituitary Gonadotropins
Haplorhini
Antibodies
Serum
Leydig Cells
Menstrual Cycle
Ovulation
Biological Assay
Progesterone
Immune Sera
Luteal Phase
Cross Reactions
Testosterone
Sheep
Vaccines

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Citar esto

Das, C. ; Talwar, G. P. ; Ramakrishnan, S. ; Salahuddin, M. ; Kumar, S. ; Hingorani, V. ; Coutinho, E. ; Croxatto, H. ; Hemmingson, E. ; Johansson, E. ; Luukkainen, T. ; Shahani, S. ; Sundaram, K. ; Nash, H. ; Segal, S. J. / Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins. En: Contraception. 1978 ; Vol. 18, N.º 1. pp. 35-50.
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title = "Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins",
abstract = "Serum from monkeys and human subjects immunized with an anti-hCG vaccine, Pr-β-hCG-TT, have been analyzed for their capacity to neutralize hCG/LH induced biological effects in two test systems. The monkey antiserum blocked completely the hCG induced ovulation in mice, but the same amount of the antiserum and even two-fold higher concentrations, did not reduce the number of ovulating animals on priming with ovine LH. The immunized monkey and the human sera were competent to neutralise the hCG induced testosterone production in Leydig cells. The monkey and seven out of twelve human sera did not interfere in the hLH action on these cells. Five human sera, however, showed varying degree of inhibition of hLH induced steroidogenesis in this sensitive bioassay. These subjects nevertheless maintained regular menstrual cycles and serum progesterone levels in the luteal phase were consonant with ovulation. In a subject whose serum had shown a fairly high degree of cross-reaction with hLH in Leydig cell bioassay, estradial, hLH and progesterone levels were determined on different days throughout a menstrual cycle. The hormonal profiles were normal.",
author = "C. Das and Talwar, {G. P.} and S. Ramakrishnan and M. Salahuddin and S. Kumar and V. Hingorani and E. Coutinho and H. Croxatto and E. Hemmingson and E. Johansson and T. Luukkainen and S. Shahani and K. Sundaram and H. Nash and Segal, {S. J.}",
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Das, C, Talwar, GP, Ramakrishnan, S, Salahuddin, M, Kumar, S, Hingorani, V, Coutinho, E, Croxatto, H, Hemmingson, E, Johansson, E, Luukkainen, T, Shahani, S, Sundaram, K, Nash, H & Segal, SJ 1978, 'Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins', Contraception, vol. 18, n.º 1, pp. 35-50. https://doi.org/10.1016/0010-7824(78)90100-2

Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins. / Das, C.; Talwar, G. P.; Ramakrishnan, S.; Salahuddin, M.; Kumar, S.; Hingorani, V.; Coutinho, E.; Croxatto, H.; Hemmingson, E.; Johansson, E.; Luukkainen, T.; Shahani, S.; Sundaram, K.; Nash, H.; Segal, S. J.

En: Contraception, Vol. 18, N.º 1, 01.01.1978, p. 35-50.

Resultado de la investigación: Article

TY - JOUR

T1 - Discriminatory effect of anti-Pr-β-hCG-TT antibodies on the neutralization of the biological activity of placental and pituitary gonadotropins

AU - Das, C.

AU - Talwar, G. P.

AU - Ramakrishnan, S.

AU - Salahuddin, M.

AU - Kumar, S.

AU - Hingorani, V.

AU - Coutinho, E.

AU - Croxatto, H.

AU - Hemmingson, E.

AU - Johansson, E.

AU - Luukkainen, T.

AU - Shahani, S.

AU - Sundaram, K.

AU - Nash, H.

AU - Segal, S. J.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Serum from monkeys and human subjects immunized with an anti-hCG vaccine, Pr-β-hCG-TT, have been analyzed for their capacity to neutralize hCG/LH induced biological effects in two test systems. The monkey antiserum blocked completely the hCG induced ovulation in mice, but the same amount of the antiserum and even two-fold higher concentrations, did not reduce the number of ovulating animals on priming with ovine LH. The immunized monkey and the human sera were competent to neutralise the hCG induced testosterone production in Leydig cells. The monkey and seven out of twelve human sera did not interfere in the hLH action on these cells. Five human sera, however, showed varying degree of inhibition of hLH induced steroidogenesis in this sensitive bioassay. These subjects nevertheless maintained regular menstrual cycles and serum progesterone levels in the luteal phase were consonant with ovulation. In a subject whose serum had shown a fairly high degree of cross-reaction with hLH in Leydig cell bioassay, estradial, hLH and progesterone levels were determined on different days throughout a menstrual cycle. The hormonal profiles were normal.

AB - Serum from monkeys and human subjects immunized with an anti-hCG vaccine, Pr-β-hCG-TT, have been analyzed for their capacity to neutralize hCG/LH induced biological effects in two test systems. The monkey antiserum blocked completely the hCG induced ovulation in mice, but the same amount of the antiserum and even two-fold higher concentrations, did not reduce the number of ovulating animals on priming with ovine LH. The immunized monkey and the human sera were competent to neutralise the hCG induced testosterone production in Leydig cells. The monkey and seven out of twelve human sera did not interfere in the hLH action on these cells. Five human sera, however, showed varying degree of inhibition of hLH induced steroidogenesis in this sensitive bioassay. These subjects nevertheless maintained regular menstrual cycles and serum progesterone levels in the luteal phase were consonant with ovulation. In a subject whose serum had shown a fairly high degree of cross-reaction with hLH in Leydig cell bioassay, estradial, hLH and progesterone levels were determined on different days throughout a menstrual cycle. The hormonal profiles were normal.

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U2 - 10.1016/0010-7824(78)90100-2

DO - 10.1016/0010-7824(78)90100-2

M3 - Article

C2 - 98287

AN - SCOPUS:0017850779

VL - 18

SP - 35

EP - 50

JO - Contraception

JF - Contraception

SN - 0010-7824

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