Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado

Carlos Herrera, Maria Tello, Ignacio Ibanez, Luis Velasquez, Marcelo Mancini, Sandra Alvarez

Resultado de la investigación: Article

Resumen

Hydrolysis of albumin (BSA), as a model protein, in 6N HCI inside a PFA screw capped sample vial, was analysed at diferent times by heating in a household microwave oven. The protein hydrolisis reaction times were followed through molecular filtration chromatography, by measuring in a UV monitor at 254 nm, the decreasing in protein and increasing in free amino acids (tyrosine) peak's absorbances, of the samples eluted from a Sephadex G-25 column. The total hydrolysis of the protein was achieved after 30 minutes and at a half of the nominal power of the microwave oven (approx. 300 W). The method was succesfully applied to the hydrolysis of the protein present in fish meal, as previous preparation of the sample, for later determination of basic amino acids and biogenic amines, by ion exchange chromatography, followed by HPLC.

Idioma originalSpanish
Páginas (desde-hasta)487-494
Número de páginas8
PublicaciónBoletin de la Sociedad Chilena de Quimica
Volumen46
N.º4
EstadoPublished - 2001

ASJC Scopus subject areas

  • Chemistry(all)

Citar esto

Herrera, C., Tello, M., Ibanez, I., Velasquez, L., Mancini, M., & Alvarez, S. (2001). Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado. Boletin de la Sociedad Chilena de Quimica, 46(4), 487-494.
Herrera, Carlos ; Tello, Maria ; Ibanez, Ignacio ; Velasquez, Luis ; Mancini, Marcelo ; Alvarez, Sandra. / Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado. En: Boletin de la Sociedad Chilena de Quimica. 2001 ; Vol. 46, N.º 4. pp. 487-494.
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Herrera, C, Tello, M, Ibanez, I, Velasquez, L, Mancini, M & Alvarez, S 2001, 'Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado', Boletin de la Sociedad Chilena de Quimica, vol. 46, n.º 4, pp. 487-494.

Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado. / Herrera, Carlos; Tello, Maria; Ibanez, Ignacio; Velasquez, Luis; Mancini, Marcelo; Alvarez, Sandra.

En: Boletin de la Sociedad Chilena de Quimica, Vol. 46, N.º 4, 2001, p. 487-494.

Resultado de la investigación: Article

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T1 - Digestion proteica con microondas y su aplicaciön en preparacion de muestras para analisis de marina de pescado

AU - Herrera, Carlos

AU - Tello, Maria

AU - Ibanez, Ignacio

AU - Velasquez, Luis

AU - Mancini, Marcelo

AU - Alvarez, Sandra

PY - 2001

Y1 - 2001

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KW - Fish meal

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