Differential transport of fertilized and unfertilized ova in the rat

M. Villalon, M. E. Ortiz, C. Aguayo, J. Muñoz, H. B. Croxatto

Resultado de la investigación: Article

20 Citas (Scopus)

Resumen

A possible role of the quality of eggs in the regulation of ovum transport was investigated in pseudopregnant rats. Pseudopregnancy was induced by mechanical stimulation of the cervix at 2200 h on the day of proestrus. This was immediately followed by artificial insemination with fertile or infertile epididymal spermatozoa. The following day was designated as Day 1 of pseudopregnancy. Spermatozoa were rendered infertile by freezing and thawing. Insemination with fertile spermatozoa yielded between 80% and 100% of normally developing embryos, as judged by their microscopic appearance and by the implantation rate on Day 10 of pseudopregnancy. Insemination with infertile spermatozoa yielded only cytolyzed or fragmented ova. Animals were randomly assigned to either one of three groups: In group 1 they were inseminated in both uterine horns with fertile spermatozoa. Females in Group 2 were inseminated in both uterine horns with infertile spermatozoa and those in Group 3 were inseminated with fertile spermatozoa in one uterine horn and with infertile spermatozoa in the contralateral horn. Animals in a subgroup of Group 2 had the uterine horns ligated on Day 4. The females were sacrificed at different intervals between Days 2 and 5 of pseudopregnancy, to determine the number, condition and distribution of ova in the genital tract and to determine plasma progesterone concentrations in blood obtained from the aorta. The number and distribution of ova in Groups 1 and 2 and in both sides of Group 3 was similar until morning of Day 4, but after their passage to the uterus there was a significant loss of unfertilized ova which was prevented by placement of a ligature in the uterine horns. The differential transport of embryos and unfertilized ova was not associated with different plasma progesterone levels. On the other hand, differential transport was observed in animals transporting the two types of eggs at the same time but in different uterine horns. The results of this study indicate that ova can exert a local influence upon the uterine phase of transport which is associated with fertilization and/or development.

Idioma originalEnglish
Páginas (desde-hasta)337-341
Número de páginas5
PublicaciónBiology of Reproduction
Volumen26
N.º2
DOI
EstadoPublished - 1982

Huella dactilar

Zygote
Spermatozoa
Pseudopregnancy
Ovum
Insemination
Eggs
Progesterone
Ovum Transport
Embryonic Structures
Proestrus
Artificial Insemination
Fertilization
Cervix Uteri
Freezing
Uterus
Ligation
Aorta

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology
  • Medicine(all)

Citar esto

Villalon, M. ; Ortiz, M. E. ; Aguayo, C. ; Muñoz, J. ; Croxatto, H. B. / Differential transport of fertilized and unfertilized ova in the rat. En: Biology of Reproduction. 1982 ; Vol. 26, N.º 2. pp. 337-341.
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abstract = "A possible role of the quality of eggs in the regulation of ovum transport was investigated in pseudopregnant rats. Pseudopregnancy was induced by mechanical stimulation of the cervix at 2200 h on the day of proestrus. This was immediately followed by artificial insemination with fertile or infertile epididymal spermatozoa. The following day was designated as Day 1 of pseudopregnancy. Spermatozoa were rendered infertile by freezing and thawing. Insemination with fertile spermatozoa yielded between 80{\%} and 100{\%} of normally developing embryos, as judged by their microscopic appearance and by the implantation rate on Day 10 of pseudopregnancy. Insemination with infertile spermatozoa yielded only cytolyzed or fragmented ova. Animals were randomly assigned to either one of three groups: In group 1 they were inseminated in both uterine horns with fertile spermatozoa. Females in Group 2 were inseminated in both uterine horns with infertile spermatozoa and those in Group 3 were inseminated with fertile spermatozoa in one uterine horn and with infertile spermatozoa in the contralateral horn. Animals in a subgroup of Group 2 had the uterine horns ligated on Day 4. The females were sacrificed at different intervals between Days 2 and 5 of pseudopregnancy, to determine the number, condition and distribution of ova in the genital tract and to determine plasma progesterone concentrations in blood obtained from the aorta. The number and distribution of ova in Groups 1 and 2 and in both sides of Group 3 was similar until morning of Day 4, but after their passage to the uterus there was a significant loss of unfertilized ova which was prevented by placement of a ligature in the uterine horns. The differential transport of embryos and unfertilized ova was not associated with different plasma progesterone levels. On the other hand, differential transport was observed in animals transporting the two types of eggs at the same time but in different uterine horns. The results of this study indicate that ova can exert a local influence upon the uterine phase of transport which is associated with fertilization and/or development.",
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Differential transport of fertilized and unfertilized ova in the rat. / Villalon, M.; Ortiz, M. E.; Aguayo, C.; Muñoz, J.; Croxatto, H. B.

En: Biology of Reproduction, Vol. 26, N.º 2, 1982, p. 337-341.

Resultado de la investigación: Article

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