Differential regulation of Notch ligands in dendritic cells upon interaction with T helper cells

D. Sauma, P. Espejo, A. Ramirez, A. Fierro, M. Rosemblatt, M. R. Bono

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

The Notch signalling pathway has recently been linked to T helper 1 (Th1)/T helper 2 (Th2) cell polarization via a mechanism involving differential expression of Notch ligands, Delta-like and Jagged, in antigen-presenting cells. However, whether stimuli other than pathogen-derived factors are involved in the regulation of Notch ligand expression in dendritic cells (DCs) remains unknown. Here, we address the effect of T helper cells (Th1 and Th2) on Delta-like 4 and Jagged 2 expression in bone marrow-derived DCs. We demonstrate that both Th1 and Th2 cells induce Delta-like 4 mRNA expression in DCs, in a process that is, in part, mediated by CD40 signalling. In contrast, only Th2 cells induce a significant increase in Jagged 2 mRNA levels in DCs. Additionally, we show that IL-4, a hallmark Th2 cytokine, plays a role in Jagged 2 expression, as evidenced by the fact that cholera toxin, a Th2-promoting stimulus, induces Jagged 2 mRNA expression in DCs only in the presence of IL-4. Finally, we demonstrate that DCs also express Notch 1 and that this expression is downregulated by IL-4. These data suggest that Notch ligands are differentially regulated in DCs: Delta-like 4 is regulated by T helper cells and by pathogen-derived Th1 stimuli, whereas Jagged 2 is regulated by Th2 cells and pathogen-derived Th2-promoting stimuli. Based on our results, we propose that the positive feedback loop that Th2 cells exert on T cell polarization may involve the induction of Jagged 2 expression in DCs.

Idioma originalEnglish
Páginas (desde-hasta)62-70
Número de páginas9
PublicaciónScandinavian Journal of Immunology
Volumen74
N.º1
DOI
EstadoPublished - jul 2011

Huella dactilar

Helper-Inducer T-Lymphocytes
Cell Communication
Dendritic Cells
Th2 Cells
Ligands
Interleukin-4
Messenger RNA
Cholera Toxin
Antigen-Presenting Cells
Down-Regulation
Bone Marrow
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Citar esto

Sauma, D. ; Espejo, P. ; Ramirez, A. ; Fierro, A. ; Rosemblatt, M. ; Bono, M. R. / Differential regulation of Notch ligands in dendritic cells upon interaction with T helper cells. En: Scandinavian Journal of Immunology. 2011 ; Vol. 74, N.º 1. pp. 62-70.
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Differential regulation of Notch ligands in dendritic cells upon interaction with T helper cells. / Sauma, D.; Espejo, P.; Ramirez, A.; Fierro, A.; Rosemblatt, M.; Bono, M. R.

En: Scandinavian Journal of Immunology, Vol. 74, N.º 1, 07.2011, p. 62-70.

Resultado de la investigación: Article

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AU - Espejo, P.

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AU - Bono, M. R.

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