Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II

Pablo Aránguiz-Urroz, Dagoberto Soto, Ariel Contreras, Rodrigo Troncoso, Mario Chiong, José Montenegro, Daniel Venegas, Christian Smolic, Pedro Ayala, Walter G. Thomas, Sergio Lavandero, Guillermo Díaz-Araya

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Background: The Angiotensin II (Ang II) type 1 (AT"1R) and type 2 (AT"2R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.MethodsWe ectopically expressed AT"1R and AT"2R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.ResultsIn adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT"1R, and no effect of Ang II on cell viability was observed following AT"1R or AT"2R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT"1R (but not the AT"2R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.ConclusionsWe conclude that Ang II/AT"1R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.

Idioma originalEnglish
Páginas (desde-hasta)569-576
Número de páginas8
PublicaciónAmerican Journal of Hypertension
Volumen22
N.º5
DOI
EstadoPublished - 1 may 2009

Huella dactilar

Angiotensin Type 2 Receptor
Angiotensin Type 1 Receptor
Angiotensin II
Cell Death
Cardiac Myocytes
Fibroblasts
Cell Survival
Apoptosis
Mitochondrial Membrane Potential
Dilated Cardiomyopathy
Cardiomegaly
Hypertrophy
Myocardial Infarction
Calcium

ASJC Scopus subject areas

  • Internal Medicine

Citar esto

Aránguiz-Urroz, P., Soto, D., Contreras, A., Troncoso, R., Chiong, M., Montenegro, J., ... Díaz-Araya, G. (2009). Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II. American Journal of Hypertension, 22(5), 569-576. https://doi.org/10.1038/ajh.2009.32
Aránguiz-Urroz, Pablo ; Soto, Dagoberto ; Contreras, Ariel ; Troncoso, Rodrigo ; Chiong, Mario ; Montenegro, José ; Venegas, Daniel ; Smolic, Christian ; Ayala, Pedro ; Thomas, Walter G. ; Lavandero, Sergio ; Díaz-Araya, Guillermo. / Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II. En: American Journal of Hypertension. 2009 ; Vol. 22, N.º 5. pp. 569-576.
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title = "Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II",
abstract = "Background: The Angiotensin II (Ang II) type 1 (AT{"}1R) and type 2 (AT{"}2R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.MethodsWe ectopically expressed AT{"}1R and AT{"}2R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.ResultsIn adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT{"}1R, and no effect of Ang II on cell viability was observed following AT{"}1R or AT{"}2R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT{"}1R (but not the AT{"}2R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.ConclusionsWe conclude that Ang II/AT{"}1R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.",
author = "Pablo Ar{\'a}nguiz-Urroz and Dagoberto Soto and Ariel Contreras and Rodrigo Troncoso and Mario Chiong and Jos{\'e} Montenegro and Daniel Venegas and Christian Smolic and Pedro Ayala and Thomas, {Walter G.} and Sergio Lavandero and Guillermo D{\'i}az-Araya",
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Aránguiz-Urroz, P, Soto, D, Contreras, A, Troncoso, R, Chiong, M, Montenegro, J, Venegas, D, Smolic, C, Ayala, P, Thomas, WG, Lavandero, S & Díaz-Araya, G 2009, 'Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II', American Journal of Hypertension, vol. 22, n.º 5, pp. 569-576. https://doi.org/10.1038/ajh.2009.32

Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II. / Aránguiz-Urroz, Pablo; Soto, Dagoberto; Contreras, Ariel; Troncoso, Rodrigo; Chiong, Mario; Montenegro, José; Venegas, Daniel; Smolic, Christian; Ayala, Pedro; Thomas, Walter G.; Lavandero, Sergio; Díaz-Araya, Guillermo.

En: American Journal of Hypertension, Vol. 22, N.º 5, 01.05.2009, p. 569-576.

Resultado de la investigación: Article

TY - JOUR

T1 - Differential participation of angiotensin II type 1 and 2 receptors in the regulation of cardiac cell death triggered by angiotensin II

AU - Aránguiz-Urroz, Pablo

AU - Soto, Dagoberto

AU - Contreras, Ariel

AU - Troncoso, Rodrigo

AU - Chiong, Mario

AU - Montenegro, José

AU - Venegas, Daniel

AU - Smolic, Christian

AU - Ayala, Pedro

AU - Thomas, Walter G.

AU - Lavandero, Sergio

AU - Díaz-Araya, Guillermo

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Background: The Angiotensin II (Ang II) type 1 (AT"1R) and type 2 (AT"2R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.MethodsWe ectopically expressed AT"1R and AT"2R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.ResultsIn adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT"1R, and no effect of Ang II on cell viability was observed following AT"1R or AT"2R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT"1R (but not the AT"2R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.ConclusionsWe conclude that Ang II/AT"1R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.

AB - Background: The Angiotensin II (Ang II) type 1 (AT"1R) and type 2 (AT"2R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.MethodsWe ectopically expressed AT"1R and AT"2R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.ResultsIn adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT"1R, and no effect of Ang II on cell viability was observed following AT"1R or AT"2R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT"1R (but not the AT"2R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.ConclusionsWe conclude that Ang II/AT"1R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.

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JO - American Journal of Hypertension

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SN - 0895-7061

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