Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle

Patricia S. Diaz, Paula A. Solar, Natalia E. Juica, Pedro A. Orihuela, Hugo Cardenas, Myron Christodoulides, Renato Vargas, Luis A. Velasquez

Resultado de la investigación: Article

13 Citas (Scopus)

Resumen

Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.

Idioma originalEnglish
Número de artículo56
PublicaciónReproductive Biology and Endocrinology
Volumen10
DOI
EstadoPublished - 16 ago 2012

Huella dactilar

Fallopian Tubes
Menstrual Cycle
Extracellular Matrix
Immunohistochemistry
Matrix Metalloproteinase 3
Follicular Phase
Extracellular Matrix Proteins
Matrix Metalloproteinase 9
Gelatin
Computational Biology
Matrix Metalloproteinases
Peptide Hydrolases
Steroids
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Developmental Biology
  • Endocrinology
  • Reproductive Medicine

Citar esto

Diaz, P. S., Solar, P. A., Juica, N. E., Orihuela, P. A., Cardenas, H., Christodoulides, M., ... Velasquez, L. A. (2012). Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle. Reproductive Biology and Endocrinology, 10, [56]. https://doi.org/10.1186/1477-7827-10-56
Diaz, Patricia S. ; Solar, Paula A. ; Juica, Natalia E. ; Orihuela, Pedro A. ; Cardenas, Hugo ; Christodoulides, Myron ; Vargas, Renato ; Velasquez, Luis A. / Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle. En: Reproductive Biology and Endocrinology. 2012 ; Vol. 10.
@article{8619801819a24da48779f9a062f10bea,
title = "Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle",
abstract = "Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.",
keywords = "Extracellular matrix, Fallopian tubes, Menstrual cycle, Metalloproteinases",
author = "Diaz, {Patricia S.} and Solar, {Paula A.} and Juica, {Natalia E.} and Orihuela, {Pedro A.} and Hugo Cardenas and Myron Christodoulides and Renato Vargas and Velasquez, {Luis A.}",
year = "2012",
month = "8",
day = "16",
doi = "10.1186/1477-7827-10-56",
language = "English",
volume = "10",
journal = "Reproductive Biology and Endocrinology",
issn = "1477-7827",
publisher = "BioMed Central",

}

Diaz, PS, Solar, PA, Juica, NE, Orihuela, PA, Cardenas, H, Christodoulides, M, Vargas, R & Velasquez, LA 2012, 'Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle', Reproductive Biology and Endocrinology, vol. 10, 56. https://doi.org/10.1186/1477-7827-10-56

Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle. / Diaz, Patricia S.; Solar, Paula A.; Juica, Natalia E.; Orihuela, Pedro A.; Cardenas, Hugo; Christodoulides, Myron; Vargas, Renato; Velasquez, Luis A.

En: Reproductive Biology and Endocrinology, Vol. 10, 56, 16.08.2012.

Resultado de la investigación: Article

TY - JOUR

T1 - Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle

AU - Diaz, Patricia S.

AU - Solar, Paula A.

AU - Juica, Natalia E.

AU - Orihuela, Pedro A.

AU - Cardenas, Hugo

AU - Christodoulides, Myron

AU - Vargas, Renato

AU - Velasquez, Luis A.

PY - 2012/8/16

Y1 - 2012/8/16

N2 - Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.

AB - Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.

KW - Extracellular matrix

KW - Fallopian tubes

KW - Menstrual cycle

KW - Metalloproteinases

UR - http://www.scopus.com/inward/record.url?scp=84864962620&partnerID=8YFLogxK

U2 - 10.1186/1477-7827-10-56

DO - 10.1186/1477-7827-10-56

M3 - Article

C2 - 22897899

AN - SCOPUS:84864962620

VL - 10

JO - Reproductive Biology and Endocrinology

JF - Reproductive Biology and Endocrinology

SN - 1477-7827

M1 - 56

ER -