Differences in the immune response elicited by two immunization schedules with an inactivated SARS-CoV-2 vaccine in a randomized phase 3 clinical trial

Nicolás M.S. Gálvez, Gaspar A. Pacheco, Bárbara M. Schultz, Felipe Melo-González, Jorge A. Soto, Luisa F. Duarte, Liliana A. González, Daniela Rivera-Pérez, Mariana Ríos, Roslye V. Berrios, Yaneisi Vázquez, Daniela Moreno-Tapia, Omar P. Vallejos, Catalina A. Andrade, Guillermo Hoppe-Elsholz, Carolina Iturriaga, Marcela Urzua, María S. Navarrete, Álvaro Rojas, Rodrigo FasceJorge Fernández, Judith Mora, Eugenio Ramírez, Aracelly Gaete-Argel, Mónica L. Acevedo, Fernando Valiente-Echeverría, Ricardo Soto-Rifo, Daniela Weiskopf, Alba Grifoni, Alessandro Sette, Gang Zeng, Weining Meng, José V. González-Aramundiz, Marina Johnson, David Goldblatt, Pablo A. González, Katia Abarca, Susan M. Bueno, Alexis M. Kalergis

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3 Citas (Scopus)

Resumen

Background: The development of vaccines to control the coronavirus disease 2019 (COVID-19) pandemic progression is a worldwide priority. CoronaVac is an inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine approved for emergency use with robust efficacy and immunogenicity data reported in trials in China, Brazil, Indonesia, Turkey, and Chile. Methods: This study is a randomized, multicenter, and controlled phase 3 trial in healthy Chilean adults aged ≥18 years. Volunteers received two doses of CoronaVac separated by 2 (0–14 schedule) or 4 weeks (0–28 schedule); 2302 volunteers were enrolled, 440 were part of the immunogenicity arm, and blood samples were obtained at different times. Samples from a single center are reported. Humoral immune responses were evaluated by measuring the neutralizing capacities of circulating antibodies. Cellular immune responses were assessed by ELISPOT and flow cytometry. Correlation matrixes were performed to evaluate correlations in the data measured. Results: Both schedules exhibited robust neutralizing capacities with the response induced by the 0–28 schedule being better. No differences were found in the concentration of antibodies against the virus and different variants of concern (VOCs) between schedules. Stimulation of peripheral blood mononuclear cells (PBMCs) with Mega pools of Peptides (MPs) induced the secretion of interferon (IFN)-γ and the expression of activation induced markers in CD4+ T cells for both schedules. Correlation matrixes showed strong correlations between neutralizing antibodies and IFN-γ secretion. Conclusions: Immunization with CoronaVac in Chilean adults promotes robust cellular and humoral immune responses. The 0–28 schedule induced a stronger humoral immune response than the 0–14 schedule.

Idioma originalInglés
Número de artículoe81477
PublicacióneLife
Volumen11
DOI
EstadoPublicada - 2022

Áreas temáticas de ASJC Scopus

  • Neurociencias General
  • Bioquímica, Genética y Biología Molecular General
  • Inmunología y Microbiología General

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