Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy

Alejandro Tapia, Lisa M. Gangi, Fernando Zegers-Hochschild, José Balmaceda, Ricardo Pommer, León Trejo, Isabel Margarita Pacheco, Ana María Salvatierra, Soledad Henríquez, Marisol Quezada, MacArena Vargas, Miguel Ríos, David J. Munroe, Horacio B. Croxatto, Luis Velasquez

Resultado de la investigación: Article

58 Citas (Scopus)

Resumen

BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (≥2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.

Idioma originalEnglish
Páginas (desde-hasta)340-351
Número de páginas12
PublicaciónHuman Reproduction
Volumen23
N.º2
DOI
EstadoPublished - 1 feb 2008

Huella dactilar

Endometrium
Pregnancy
Progesterone
Oocyte Donation
Gene Expression
Gene Expression Profiling
Menstrual Cycle
Oligonucleotide Array Sequence Analysis
Matrix Metalloproteinases
Genes
Oocytes
Fertility
Real-Time Polymerase Chain Reaction
Estradiol
RNA
Biopsy
Control Groups

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Medicine(all)

Citar esto

Tapia, Alejandro ; Gangi, Lisa M. ; Zegers-Hochschild, Fernando ; Balmaceda, José ; Pommer, Ricardo ; Trejo, León ; Pacheco, Isabel Margarita ; Salvatierra, Ana María ; Henríquez, Soledad ; Quezada, Marisol ; Vargas, MacArena ; Ríos, Miguel ; Munroe, David J. ; Croxatto, Horacio B. ; Velasquez, Luis. / Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy. En: Human Reproduction. 2008 ; Vol. 23, N.º 2. pp. 340-351.
@article{7553e78c9f194543ab744a63875e8bda,
title = "Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy",
abstract = "BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (≥2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.",
keywords = "Endometrium, Implantation, Microarrays, Oocyte donation, Uterine receptivity",
author = "Alejandro Tapia and Gangi, {Lisa M.} and Fernando Zegers-Hochschild and Jos{\'e} Balmaceda and Ricardo Pommer and Le{\'o}n Trejo and Pacheco, {Isabel Margarita} and Salvatierra, {Ana Mar{\'i}a} and Soledad Henr{\'i}quez and Marisol Quezada and MacArena Vargas and Miguel R{\'i}os and Munroe, {David J.} and Croxatto, {Horacio B.} and Luis Velasquez",
year = "2008",
month = "2",
day = "1",
doi = "10.1093/humrep/dem319",
language = "English",
volume = "23",
pages = "340--351",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "2",

}

Tapia, A, Gangi, LM, Zegers-Hochschild, F, Balmaceda, J, Pommer, R, Trejo, L, Pacheco, IM, Salvatierra, AM, Henríquez, S, Quezada, M, Vargas, M, Ríos, M, Munroe, DJ, Croxatto, HB & Velasquez, L 2008, 'Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy', Human Reproduction, vol. 23, n.º 2, pp. 340-351. https://doi.org/10.1093/humrep/dem319

Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy. / Tapia, Alejandro; Gangi, Lisa M.; Zegers-Hochschild, Fernando; Balmaceda, José; Pommer, Ricardo; Trejo, León; Pacheco, Isabel Margarita; Salvatierra, Ana María; Henríquez, Soledad; Quezada, Marisol; Vargas, MacArena; Ríos, Miguel; Munroe, David J.; Croxatto, Horacio B.; Velasquez, Luis.

En: Human Reproduction, Vol. 23, N.º 2, 01.02.2008, p. 340-351.

Resultado de la investigación: Article

TY - JOUR

T1 - Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy

AU - Tapia, Alejandro

AU - Gangi, Lisa M.

AU - Zegers-Hochschild, Fernando

AU - Balmaceda, José

AU - Pommer, Ricardo

AU - Trejo, León

AU - Pacheco, Isabel Margarita

AU - Salvatierra, Ana María

AU - Henríquez, Soledad

AU - Quezada, Marisol

AU - Vargas, MacArena

AU - Ríos, Miguel

AU - Munroe, David J.

AU - Croxatto, Horacio B.

AU - Velasquez, Luis

PY - 2008/2/1

Y1 - 2008/2/1

N2 - BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (≥2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.

AB - BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (≥2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.

KW - Endometrium

KW - Implantation

KW - Microarrays

KW - Oocyte donation

KW - Uterine receptivity

UR - http://www.scopus.com/inward/record.url?scp=40349108575&partnerID=8YFLogxK

U2 - 10.1093/humrep/dem319

DO - 10.1093/humrep/dem319

M3 - Article

C2 - 18077318

AN - SCOPUS:40349108575

VL - 23

SP - 340

EP - 351

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 2

ER -