Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1

Daniel Cabrera, Alex Ruiz, Claudio Cabello-Verrugio, Enrique Brandan, Lisbell Estrada, Margarita Pizarro, Nancy Solis, Javiera Torres, Francisco Barrera, Marco Arrese

Resultado de la investigación: Article

18 Citas (Scopus)

Resumen

Background: Decreased muscle mass or sarcopenia has been associated with nonalcoholic fatty liver disease (NAFLD). However, the functional consequences of this association and its pathogenesis remain ill-defined. Aims: To evaluate muscle mass and function in a diet-induced NAFLD mouse model and explore its association with changes in serum insulin-like growth factor-1 (IGF-1). Methods: Weight gain, visceral fat, serum biochemical parameters, liver histology, and hepatic triglyceride content (HTC) were assessed in C57/Bl6 mice fed a westernized diet during 16 weeks. In addition, we determined muscle fiber size and strength of limb skeletal muscle, myosin heavy chain (MHC) protein levels, and IGF-1 serum levels. Results: Westernized diet feeding was associated with weight gain, increased visceral fat mass (epididymal pad weight: 0.76 g ± 0.13 vs. 0.33 ± 0.27 g; p = 0.0023), hepatic steatosis (HTC: 118.2 ± 6.88 mg/g liver vs. 43.26 ± 5.63 mg/g<, p < 0.05), and necroinflammation (histological scores: 1.29 ± 0.42 vs. 4.00 ± 0.53<, p < 0.05). Also, mice fed the experimental diet had an increased proportion of low-diameter muscle fibers (0–30 μm) and a decreased proportion of high-diameter muscle fibers (60–90 μm), which correlated with decreased MHC protein levels, consistent with significant muscle atrophy. Functional studies showed that mice fed a westernized diet had reduced muscle strength and lower serum levels of IGF-1 (284.2 ± 20.04 pg/ml) compared with chow-fed mice (366.0 ± 12.42 pg/ml, p < 0.05). Conclusion: Experimental NAFLD is associated with sarcopenia, decreased muscle strength, and reduced IGF-1 serum levels. IGF-1 reduction may be involved in pathogenesis of NAFLD-associated sarcopenia.

Idioma originalEnglish
Páginas (desde-hasta)3190-3198
Número de páginas9
PublicaciónDigestive Diseases and Sciences
Volumen61
N.º11
DOI
EstadoPublished - 1 nov 2016

Huella dactilar

Sarcopenia
Somatomedins
Diet
Muscles
Liver
Serum
Intra-Abdominal Fat
Myosin Heavy Chains
Muscle Strength
Weight Gain
Triglycerides
Skeletal Muscle Myosins
Muscular Atrophy
Histology
Proteins
Extremities
Non-alcoholic Fatty Liver Disease
Weights and Measures

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Citar esto

Cabrera, Daniel ; Ruiz, Alex ; Cabello-Verrugio, Claudio ; Brandan, Enrique ; Estrada, Lisbell ; Pizarro, Margarita ; Solis, Nancy ; Torres, Javiera ; Barrera, Francisco ; Arrese, Marco. / Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1. En: Digestive Diseases and Sciences. 2016 ; Vol. 61, N.º 11. pp. 3190-3198.
@article{d4db0c74ee7e4fd9af7a5b319fd1842a,
title = "Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1",
abstract = "Background: Decreased muscle mass or sarcopenia has been associated with nonalcoholic fatty liver disease (NAFLD). However, the functional consequences of this association and its pathogenesis remain ill-defined. Aims: To evaluate muscle mass and function in a diet-induced NAFLD mouse model and explore its association with changes in serum insulin-like growth factor-1 (IGF-1). Methods: Weight gain, visceral fat, serum biochemical parameters, liver histology, and hepatic triglyceride content (HTC) were assessed in C57/Bl6 mice fed a westernized diet during 16 weeks. In addition, we determined muscle fiber size and strength of limb skeletal muscle, myosin heavy chain (MHC) protein levels, and IGF-1 serum levels. Results: Westernized diet feeding was associated with weight gain, increased visceral fat mass (epididymal pad weight: 0.76 g ± 0.13 vs. 0.33 ± 0.27 g; p = 0.0023), hepatic steatosis (HTC: 118.2 ± 6.88 mg/g liver vs. 43.26 ± 5.63 mg/g<, p < 0.05), and necroinflammation (histological scores: 1.29 ± 0.42 vs. 4.00 ± 0.53<, p < 0.05). Also, mice fed the experimental diet had an increased proportion of low-diameter muscle fibers (0–30 μm) and a decreased proportion of high-diameter muscle fibers (60–90 μm), which correlated with decreased MHC protein levels, consistent with significant muscle atrophy. Functional studies showed that mice fed a westernized diet had reduced muscle strength and lower serum levels of IGF-1 (284.2 ± 20.04 pg/ml) compared with chow-fed mice (366.0 ± 12.42 pg/ml, p < 0.05). Conclusion: Experimental NAFLD is associated with sarcopenia, decreased muscle strength, and reduced IGF-1 serum levels. IGF-1 reduction may be involved in pathogenesis of NAFLD-associated sarcopenia.",
keywords = "Fructose, Insulin, Muscular atrophy, Nonalcoholic fatty liver disease, Nonalcoholic steatohepatitis, Sarcopenia",
author = "Daniel Cabrera and Alex Ruiz and Claudio Cabello-Verrugio and Enrique Brandan and Lisbell Estrada and Margarita Pizarro and Nancy Solis and Javiera Torres and Francisco Barrera and Marco Arrese",
year = "2016",
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language = "English",
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pages = "3190--3198",
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Cabrera, D, Ruiz, A, Cabello-Verrugio, C, Brandan, E, Estrada, L, Pizarro, M, Solis, N, Torres, J, Barrera, F & Arrese, M 2016, 'Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1', Digestive Diseases and Sciences, vol. 61, n.º 11, pp. 3190-3198. https://doi.org/10.1007/s10620-016-4285-0

Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1. / Cabrera, Daniel; Ruiz, Alex; Cabello-Verrugio, Claudio; Brandan, Enrique; Estrada, Lisbell; Pizarro, Margarita; Solis, Nancy; Torres, Javiera; Barrera, Francisco; Arrese, Marco.

En: Digestive Diseases and Sciences, Vol. 61, N.º 11, 01.11.2016, p. 3190-3198.

Resultado de la investigación: Article

TY - JOUR

T1 - Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1

AU - Cabrera, Daniel

AU - Ruiz, Alex

AU - Cabello-Verrugio, Claudio

AU - Brandan, Enrique

AU - Estrada, Lisbell

AU - Pizarro, Margarita

AU - Solis, Nancy

AU - Torres, Javiera

AU - Barrera, Francisco

AU - Arrese, Marco

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Background: Decreased muscle mass or sarcopenia has been associated with nonalcoholic fatty liver disease (NAFLD). However, the functional consequences of this association and its pathogenesis remain ill-defined. Aims: To evaluate muscle mass and function in a diet-induced NAFLD mouse model and explore its association with changes in serum insulin-like growth factor-1 (IGF-1). Methods: Weight gain, visceral fat, serum biochemical parameters, liver histology, and hepatic triglyceride content (HTC) were assessed in C57/Bl6 mice fed a westernized diet during 16 weeks. In addition, we determined muscle fiber size and strength of limb skeletal muscle, myosin heavy chain (MHC) protein levels, and IGF-1 serum levels. Results: Westernized diet feeding was associated with weight gain, increased visceral fat mass (epididymal pad weight: 0.76 g ± 0.13 vs. 0.33 ± 0.27 g; p = 0.0023), hepatic steatosis (HTC: 118.2 ± 6.88 mg/g liver vs. 43.26 ± 5.63 mg/g<, p < 0.05), and necroinflammation (histological scores: 1.29 ± 0.42 vs. 4.00 ± 0.53<, p < 0.05). Also, mice fed the experimental diet had an increased proportion of low-diameter muscle fibers (0–30 μm) and a decreased proportion of high-diameter muscle fibers (60–90 μm), which correlated with decreased MHC protein levels, consistent with significant muscle atrophy. Functional studies showed that mice fed a westernized diet had reduced muscle strength and lower serum levels of IGF-1 (284.2 ± 20.04 pg/ml) compared with chow-fed mice (366.0 ± 12.42 pg/ml, p < 0.05). Conclusion: Experimental NAFLD is associated with sarcopenia, decreased muscle strength, and reduced IGF-1 serum levels. IGF-1 reduction may be involved in pathogenesis of NAFLD-associated sarcopenia.

AB - Background: Decreased muscle mass or sarcopenia has been associated with nonalcoholic fatty liver disease (NAFLD). However, the functional consequences of this association and its pathogenesis remain ill-defined. Aims: To evaluate muscle mass and function in a diet-induced NAFLD mouse model and explore its association with changes in serum insulin-like growth factor-1 (IGF-1). Methods: Weight gain, visceral fat, serum biochemical parameters, liver histology, and hepatic triglyceride content (HTC) were assessed in C57/Bl6 mice fed a westernized diet during 16 weeks. In addition, we determined muscle fiber size and strength of limb skeletal muscle, myosin heavy chain (MHC) protein levels, and IGF-1 serum levels. Results: Westernized diet feeding was associated with weight gain, increased visceral fat mass (epididymal pad weight: 0.76 g ± 0.13 vs. 0.33 ± 0.27 g; p = 0.0023), hepatic steatosis (HTC: 118.2 ± 6.88 mg/g liver vs. 43.26 ± 5.63 mg/g<, p < 0.05), and necroinflammation (histological scores: 1.29 ± 0.42 vs. 4.00 ± 0.53<, p < 0.05). Also, mice fed the experimental diet had an increased proportion of low-diameter muscle fibers (0–30 μm) and a decreased proportion of high-diameter muscle fibers (60–90 μm), which correlated with decreased MHC protein levels, consistent with significant muscle atrophy. Functional studies showed that mice fed a westernized diet had reduced muscle strength and lower serum levels of IGF-1 (284.2 ± 20.04 pg/ml) compared with chow-fed mice (366.0 ± 12.42 pg/ml, p < 0.05). Conclusion: Experimental NAFLD is associated with sarcopenia, decreased muscle strength, and reduced IGF-1 serum levels. IGF-1 reduction may be involved in pathogenesis of NAFLD-associated sarcopenia.

KW - Fructose

KW - Insulin

KW - Muscular atrophy

KW - Nonalcoholic fatty liver disease

KW - Nonalcoholic steatohepatitis

KW - Sarcopenia

UR - http://www.scopus.com/inward/record.url?scp=84984596140&partnerID=8YFLogxK

U2 - 10.1007/s10620-016-4285-0

DO - 10.1007/s10620-016-4285-0

M3 - Article

AN - SCOPUS:84984596140

VL - 61

SP - 3190

EP - 3198

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 11

ER -