Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

T. Kurobe, K. T. Kwak, E. MacConnell, T. S. McDowell, F. O. Mardones, R. P. Hedrick

Resultado de la investigación: Article

10 Citas (Scopus)

Resumen

The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.

Idioma originalEnglish
Páginas (desde-hasta)31-42
Número de páginas12
PublicaciónDiseases of Aquatic Organisms
Volumen93
N.º1
DOI
EstadoPublished - 7 dic 2010

Huella dactilar

Iridovirus
Missouri River
captive population
sturgeon
wild population
assay
assays
virus
river
infection
electron microscopy
hatchery
viruses
viral disease
hatcheries
DNA
cytoplasm
rearing
microscopy
virion

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Citar esto

Kurobe, T. ; Kwak, K. T. ; MacConnell, E. ; McDowell, T. S. ; Mardones, F. O. ; Hedrick, R. P. / Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon. En: Diseases of Aquatic Organisms. 2010 ; Vol. 93, N.º 1. pp. 31-42.
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abstract = "The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.",
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Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon. / Kurobe, T.; Kwak, K. T.; MacConnell, E.; McDowell, T. S.; Mardones, F. O.; Hedrick, R. P.

En: Diseases of Aquatic Organisms, Vol. 93, N.º 1, 07.12.2010, p. 31-42.

Resultado de la investigación: Article

TY - JOUR

T1 - Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon

AU - Kurobe, T.

AU - Kwak, K. T.

AU - MacConnell, E.

AU - McDowell, T. S.

AU - Mardones, F. O.

AU - Hedrick, R. P.

PY - 2010/12/7

Y1 - 2010/12/7

N2 - The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.

AB - The Missouri River sturgeon iridovirus (MRSIV) is an important factor contributing to losses during the hatchery rearing of juvenile pallid Scaphirhynchus albus and shovelnose S. platorynchus sturgeon. As the virus has not been isolated in cell culture, current detection procedures rely upon a combination of light and electron microscopy. Detection of characteristic virus-infected cells in the integument, usually of the fins, in hematoxylin and eosin (H&E)-stained tissue sections provides a presumptive finding. Confirmation requires observation by electron microscopy of characteristic doubly enveloped hexagonal virions of the appropriate size in the host cell cytoplasm. To improve these diagnostic procedures, a conventional polymerase chain reduction (PCR) assay was developed as a sensitive and specific method for detection of MRSIV DNA as found in numerous tissues of both naturally and experimentally infected pallid and shovelnose sturgeon. Sequences of amplicons obtained from testing of wild-caught shovelnose sturgeon and juvenile pallid sturgeon during hatchery outbreaks were identical, suggesting that the viruses found in both sturgeon are similar or closely related. In addition, a TaqMan PCR was developed that allowed estimates of the concentrations of MRSIV DNA present in the tissues of pallid and shovelnose sturgeon during acute and persistent infection. These new PCR assays are improved methods to detect MRSIV, but equally importantly, they provide insights into to the biology of the agent for more effective management of viral diseases in captive and wild Missouri River sturgeon populations.

KW - Diagnostic PCR

KW - Fish iridovirus

KW - Missouri River sturgeon iridovirus

KW - MRSIV

KW - Pallid sturgeon

KW - Real-time PCR

KW - Shovelnose sturgeon

UR - http://www.scopus.com/inward/record.url?scp=78651513084&partnerID=8YFLogxK

U2 - 10.3354/dao02284

DO - 10.3354/dao02284

M3 - Article

C2 - 21290894

AN - SCOPUS:78651513084

VL - 93

SP - 31

EP - 42

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 1

ER -