Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant

Andrea Rivas-Aravena, Yazmin Fuentes, Julio Cartagena, Tania Brito, Verónica Poggio, José La Torre, Hegaly Mendoza, Fernando Gonzalez-Nilo, Ana María Sandino, Eugenio Spencer

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

27 Citas (Scopus)

Resumen

Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.

Idioma originalInglés
Páginas (desde-hasta)157-166
Número de páginas10
PublicaciónFish and Shellfish Immunology
Volumen45
N.º1
DOI
EstadoPublicada - 1 jul 2015

Áreas temáticas de ASJC Scopus

  • Química ambiental
  • Ciencias acuáticas

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