Development of a molecular marker for self-compatible S4′ haplotype in sweet cherry (Prunus avium L.) using high-resolution melting

Claudia Muñoz-Espinoza, Evelyn Espinosa, Roberto Bascuñán, Sebastián Tapia, Claudio Meneses, Andréa Miyasaka Almeida

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

Sweet cherry (Prunus avium L.) has stylar gametophytic self-incompatibility, which is controlled by the multi-allelic S-locus and encompasses the highly polymorphic genes for the S-ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB), which are female and male determinants, respectively. The self-compatible mutant SFB4′ corresponds to an allele variant of SFB4 and presents a frameshift mutation. Even though male-determinant molecular markers can discriminate between SFB4 and SFB4′ alleles, the methods required are laborious, time-consuming and expensive, and not suitable for massive analysis and integration into breeding programmes. Our aim was to develop molecular markers for the evaluation of self-compatibility alleles in sweet cherry, that could be used as a high-throughput screening strategy to identify SFB4 and SFB4′ alleles, based on a marker for male determinacy. Our results were consistent using primers flanking the mutation responsible for the SFB4′ allele. We designed a specific molecular marker and confirmed it in sweet cherry commercial varieties. This new molecular marker is feasible for self-compatibility alleles in the male determinant in sweet cherry-assisted breeding programs.

Idioma originalInglés
Páginas (desde-hasta)987-993
Número de páginas7
PublicaciónPlant Breeding
Volumen136
N.º6
DOI
EstadoPublicada - 1 dic 2017

Áreas temáticas de ASJC Scopus

  • Agronomía y cultivos
  • Genética
  • Botánica

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