Development of a molecular marker for self-compatible S4′ haplotype in sweet cherry (Prunus avium L.) using high-resolution melting

Claudia Muñoz-Espinoza, Evelyn Espinosa, Roberto Bascuñán, Sebastián Tapia, Claudio Meneses, Andréa Miyasaka Almeida

Resultado de la investigación: Article

Resumen

Sweet cherry (Prunus avium L.) has stylar gametophytic self-incompatibility, which is controlled by the multi-allelic S-locus and encompasses the highly polymorphic genes for the S-ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB), which are female and male determinants, respectively. The self-compatible mutant SFB4′ corresponds to an allele variant of SFB4 and presents a frameshift mutation. Even though male-determinant molecular markers can discriminate between SFB4 and SFB4′ alleles, the methods required are laborious, time-consuming and expensive, and not suitable for massive analysis and integration into breeding programmes. Our aim was to develop molecular markers for the evaluation of self-compatibility alleles in sweet cherry, that could be used as a high-throughput screening strategy to identify SFB4 and SFB4′ alleles, based on a marker for male determinacy. Our results were consistent using primers flanking the mutation responsible for the SFB4′ allele. We designed a specific molecular marker and confirmed it in sweet cherry commercial varieties. This new molecular marker is feasible for self-compatibility alleles in the male determinant in sweet cherry-assisted breeding programs.

Idioma originalEnglish
Páginas (desde-hasta)987-993
Número de páginas7
PublicaciónPlant Breeding
Volumen136
N.º6
DOI
EstadoPublished - 1 dic 2017

Huella dactilar

Prunus avium
melting
Haplotypes
Freezing
haplotypes
Alleles
alleles
genetic markers
Breeding
frameshift mutation
Frameshift Mutation
ribonucleases
breeding
screening
mutation
mutants
Mutation
loci
Genes
genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Citar esto

Muñoz-Espinoza, Claudia ; Espinosa, Evelyn ; Bascuñán, Roberto ; Tapia, Sebastián ; Meneses, Claudio ; Almeida, Andréa Miyasaka. / Development of a molecular marker for self-compatible S4′ haplotype in sweet cherry (Prunus avium L.) using high-resolution melting. En: Plant Breeding. 2017 ; Vol. 136, N.º 6. pp. 987-993.
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abstract = "Sweet cherry (Prunus avium L.) has stylar gametophytic self-incompatibility, which is controlled by the multi-allelic S-locus and encompasses the highly polymorphic genes for the S-ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB), which are female and male determinants, respectively. The self-compatible mutant SFB4′ corresponds to an allele variant of SFB4 and presents a frameshift mutation. Even though male-determinant molecular markers can discriminate between SFB4 and SFB4′ alleles, the methods required are laborious, time-consuming and expensive, and not suitable for massive analysis and integration into breeding programmes. Our aim was to develop molecular markers for the evaluation of self-compatibility alleles in sweet cherry, that could be used as a high-throughput screening strategy to identify SFB4 and SFB4′ alleles, based on a marker for male determinacy. Our results were consistent using primers flanking the mutation responsible for the SFB4′ allele. We designed a specific molecular marker and confirmed it in sweet cherry commercial varieties. This new molecular marker is feasible for self-compatibility alleles in the male determinant in sweet cherry-assisted breeding programs.",
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Development of a molecular marker for self-compatible S4′ haplotype in sweet cherry (Prunus avium L.) using high-resolution melting. / Muñoz-Espinoza, Claudia; Espinosa, Evelyn; Bascuñán, Roberto; Tapia, Sebastián; Meneses, Claudio; Almeida, Andréa Miyasaka.

En: Plant Breeding, Vol. 136, N.º 6, 01.12.2017, p. 987-993.

Resultado de la investigación: Article

TY - JOUR

T1 - Development of a molecular marker for self-compatible S4′ haplotype in sweet cherry (Prunus avium L.) using high-resolution melting

AU - Muñoz-Espinoza, Claudia

AU - Espinosa, Evelyn

AU - Bascuñán, Roberto

AU - Tapia, Sebastián

AU - Meneses, Claudio

AU - Almeida, Andréa Miyasaka

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N2 - Sweet cherry (Prunus avium L.) has stylar gametophytic self-incompatibility, which is controlled by the multi-allelic S-locus and encompasses the highly polymorphic genes for the S-ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB), which are female and male determinants, respectively. The self-compatible mutant SFB4′ corresponds to an allele variant of SFB4 and presents a frameshift mutation. Even though male-determinant molecular markers can discriminate between SFB4 and SFB4′ alleles, the methods required are laborious, time-consuming and expensive, and not suitable for massive analysis and integration into breeding programmes. Our aim was to develop molecular markers for the evaluation of self-compatibility alleles in sweet cherry, that could be used as a high-throughput screening strategy to identify SFB4 and SFB4′ alleles, based on a marker for male determinacy. Our results were consistent using primers flanking the mutation responsible for the SFB4′ allele. We designed a specific molecular marker and confirmed it in sweet cherry commercial varieties. This new molecular marker is feasible for self-compatibility alleles in the male determinant in sweet cherry-assisted breeding programs.

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