Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Matthew C. Fisher, Pria Ghosh, Jennifer M.G. Shelton, Kieran Bates, Lola Brookes, Claudia Wierzbicki, Gonçalo M. Rosa, Rhys A. Farrer, David M. Aanensen, Mario Alvarado-Rybak, Arnaud Bataille, Lee Berger, Susanne Böll, Jaime Bosch, Frances C. Clare, Elodie A. Courtois, Angelica Crottini, Andrew A. Cunningham, Thomas M. Doherty-Bone, Fikirte GebresenbetDavid J. Gower, Jacob Höglund, Timothy Y. James, Thomas S. Jenkinson, Tiffany A. Kosch, Carolina Lambertini, Anssi Laurila, Chun Fu Lin, Adeline Loyau, An Martel, Sara Meurling, Claude Miaud, Pete Minting, Serge Ndriantsoa, Simon J. O'Hanlon, Frank Pasmans, Tsanta Rakotonanahary, Falitiana C.E. Rabemananjara, Luisa P. Ribeiro, Dirk S. Schmeller, Benedikt R. Schmidt, Lee Skerratt, Freya Smith, Claudio Soto-Azat, Giulia Tessa, Luís Felipe Toledo, Andrés Valenzuela-Sánchez, Ruhan Verster, Judit Vörös, Bruce Waldman

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.

Idioma originalEnglish
Número de artículo7772
PublicaciónScientific Reports
Volumen8
N.º1
DOI
EstadoPublished - 1 dic 2018

Huella dactilar

amphibians
fungi
tadpoles
pathogens
mouthparts
methodology
biopsy
researchers
animals

ASJC Scopus subject areas

  • General

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Fisher, Matthew C. ; Ghosh, Pria ; Shelton, Jennifer M.G. ; Bates, Kieran ; Brookes, Lola ; Wierzbicki, Claudia ; Rosa, Gonçalo M. ; Farrer, Rhys A. ; Aanensen, David M. ; Alvarado-Rybak, Mario ; Bataille, Arnaud ; Berger, Lee ; Böll, Susanne ; Bosch, Jaime ; Clare, Frances C. ; Courtois, Elodie A. ; Crottini, Angelica ; Cunningham, Andrew A. ; Doherty-Bone, Thomas M. ; Gebresenbet, Fikirte ; Gower, David J. ; Höglund, Jacob ; James, Timothy Y. ; Jenkinson, Thomas S. ; Kosch, Tiffany A. ; Lambertini, Carolina ; Laurila, Anssi ; Lin, Chun Fu ; Loyau, Adeline ; Martel, An ; Meurling, Sara ; Miaud, Claude ; Minting, Pete ; Ndriantsoa, Serge ; O'Hanlon, Simon J. ; Pasmans, Frank ; Rakotonanahary, Tsanta ; Rabemananjara, Falitiana C.E. ; Ribeiro, Luisa P. ; Schmeller, Dirk S. ; Schmidt, Benedikt R. ; Skerratt, Lee ; Smith, Freya ; Soto-Azat, Claudio ; Tessa, Giulia ; Toledo, Luís Felipe ; Valenzuela-Sánchez, Andrés ; Verster, Ruhan ; Vörös, Judit ; Waldman, Bruce. / Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. En: Scientific Reports. 2018 ; Vol. 8, N.º 1.
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title = "Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi",
abstract = "Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18{\%} of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43{\%} of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.",
author = "Fisher, {Matthew C.} and Pria Ghosh and Shelton, {Jennifer M.G.} and Kieran Bates and Lola Brookes and Claudia Wierzbicki and Rosa, {Gon{\cc}alo M.} and Farrer, {Rhys A.} and Aanensen, {David M.} and Mario Alvarado-Rybak and Arnaud Bataille and Lee Berger and Susanne B{\"o}ll and Jaime Bosch and Clare, {Frances C.} and Courtois, {Elodie A.} and Angelica Crottini and Cunningham, {Andrew A.} and Doherty-Bone, {Thomas M.} and Fikirte Gebresenbet and Gower, {David J.} and Jacob H{\"o}glund and James, {Timothy Y.} and Jenkinson, {Thomas S.} and Kosch, {Tiffany A.} and Carolina Lambertini and Anssi Laurila and Lin, {Chun Fu} and Adeline Loyau and An Martel and Sara Meurling and Claude Miaud and Pete Minting and Serge Ndriantsoa and O'Hanlon, {Simon J.} and Frank Pasmans and Tsanta Rakotonanahary and Rabemananjara, {Falitiana C.E.} and Ribeiro, {Luisa P.} and Schmeller, {Dirk S.} and Schmidt, {Benedikt R.} and Lee Skerratt and Freya Smith and Claudio Soto-Azat and Giulia Tessa and Toledo, {Lu{\'i}s Felipe} and Andr{\'e}s Valenzuela-S{\'a}nchez and Ruhan Verster and Judit V{\"o}r{\"o}s and Bruce Waldman",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-24472-2",
language = "English",
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journal = "Scientific Reports",
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Fisher, MC, Ghosh, P, Shelton, JMG, Bates, K, Brookes, L, Wierzbicki, C, Rosa, GM, Farrer, RA, Aanensen, DM, Alvarado-Rybak, M, Bataille, A, Berger, L, Böll, S, Bosch, J, Clare, FC, Courtois, EA, Crottini, A, Cunningham, AA, Doherty-Bone, TM, Gebresenbet, F, Gower, DJ, Höglund, J, James, TY, Jenkinson, TS, Kosch, TA, Lambertini, C, Laurila, A, Lin, CF, Loyau, A, Martel, A, Meurling, S, Miaud, C, Minting, P, Ndriantsoa, S, O'Hanlon, SJ, Pasmans, F, Rakotonanahary, T, Rabemananjara, FCE, Ribeiro, LP, Schmeller, DS, Schmidt, BR, Skerratt, L, Smith, F, Soto-Azat, C, Tessa, G, Toledo, LF, Valenzuela-Sánchez, A, Verster, R, Vörös, J & Waldman, B 2018, 'Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi', Scientific Reports, vol. 8, n.º 1, 7772. https://doi.org/10.1038/s41598-018-24472-2

Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. / Fisher, Matthew C.; Ghosh, Pria; Shelton, Jennifer M.G.; Bates, Kieran; Brookes, Lola; Wierzbicki, Claudia; Rosa, Gonçalo M.; Farrer, Rhys A.; Aanensen, David M.; Alvarado-Rybak, Mario; Bataille, Arnaud; Berger, Lee; Böll, Susanne; Bosch, Jaime; Clare, Frances C.; Courtois, Elodie A.; Crottini, Angelica; Cunningham, Andrew A.; Doherty-Bone, Thomas M.; Gebresenbet, Fikirte; Gower, David J.; Höglund, Jacob; James, Timothy Y.; Jenkinson, Thomas S.; Kosch, Tiffany A.; Lambertini, Carolina; Laurila, Anssi; Lin, Chun Fu; Loyau, Adeline; Martel, An; Meurling, Sara; Miaud, Claude; Minting, Pete; Ndriantsoa, Serge; O'Hanlon, Simon J.; Pasmans, Frank; Rakotonanahary, Tsanta; Rabemananjara, Falitiana C.E.; Ribeiro, Luisa P.; Schmeller, Dirk S.; Schmidt, Benedikt R.; Skerratt, Lee; Smith, Freya; Soto-Azat, Claudio; Tessa, Giulia; Toledo, Luís Felipe; Valenzuela-Sánchez, Andrés; Verster, Ruhan; Vörös, Judit; Waldman, Bruce.

En: Scientific Reports, Vol. 8, N.º 1, 7772, 01.12.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

AU - Fisher, Matthew C.

AU - Ghosh, Pria

AU - Shelton, Jennifer M.G.

AU - Bates, Kieran

AU - Brookes, Lola

AU - Wierzbicki, Claudia

AU - Rosa, Gonçalo M.

AU - Farrer, Rhys A.

AU - Aanensen, David M.

AU - Alvarado-Rybak, Mario

AU - Bataille, Arnaud

AU - Berger, Lee

AU - Böll, Susanne

AU - Bosch, Jaime

AU - Clare, Frances C.

AU - Courtois, Elodie A.

AU - Crottini, Angelica

AU - Cunningham, Andrew A.

AU - Doherty-Bone, Thomas M.

AU - Gebresenbet, Fikirte

AU - Gower, David J.

AU - Höglund, Jacob

AU - James, Timothy Y.

AU - Jenkinson, Thomas S.

AU - Kosch, Tiffany A.

AU - Lambertini, Carolina

AU - Laurila, Anssi

AU - Lin, Chun Fu

AU - Loyau, Adeline

AU - Martel, An

AU - Meurling, Sara

AU - Miaud, Claude

AU - Minting, Pete

AU - Ndriantsoa, Serge

AU - O'Hanlon, Simon J.

AU - Pasmans, Frank

AU - Rakotonanahary, Tsanta

AU - Rabemananjara, Falitiana C.E.

AU - Ribeiro, Luisa P.

AU - Schmeller, Dirk S.

AU - Schmidt, Benedikt R.

AU - Skerratt, Lee

AU - Smith, Freya

AU - Soto-Azat, Claudio

AU - Tessa, Giulia

AU - Toledo, Luís Felipe

AU - Valenzuela-Sánchez, Andrés

AU - Verster, Ruhan

AU - Vörös, Judit

AU - Waldman, Bruce

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.

AB - Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.

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DO - 10.1038/s41598-018-24472-2

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VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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