Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies

Juan Aguayo, Alvaro Miquel, Naddim Aranki, Adolfo Jamett, Pablo D.T. Valenzuela, Luis O. Burzio

Resultado de la investigación: Article

20 Citas (Scopus)

Resumen

An enzyme-linked immunosorbent assay (ELISA) for the detection of Piscirickettsia salmonis in fish tissue samples was developed. The test uses a combination of different monoclonal antibodies specific against P. salmonis in the capture step of the assay. The antibodies 7G4, 6E2 and 2C1 chosen for the capture step are bound to the solid support with an adhesive protein purified from a bivalve mollusc, resulting in a high yield of adsorption and binding stability. The monoclonal antibody 7G4, used as a second antibody, is conjugated to horseradish peroxidase. The resulting ELISA test detected 7 different isolates of P. salmonis and does not cross-react with several other fish pathogens, revealing a high specificity and sensitivity. The test also detects P. salmonis in kidney tissue of infected coho salmon with 98% correlation with the immunofluorescence assay.

Idioma originalEnglish
Páginas (desde-hasta)33-38
Número de páginas6
PublicaciónDiseases of Aquatic Organisms
Volumen49
N.º1
EstadoPublished - 24 abr 2002

Huella dactilar

Piscirickettsia salmonis
antibody
monoclonal antibodies
enzyme-linked immunosorbent assay
assay
enzyme
fish
antibodies
Oncorhynchus kisutch
testing
assays
adhesives
mollusc
fluorescent antibody technique
bivalve
Bivalvia
peroxidase
adsorption
pathogen
kidneys

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Citar esto

Aguayo, J., Miquel, A., Aranki, N., Jamett, A., Valenzuela, P. D. T., & Burzio, L. O. (2002). Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies. Diseases of Aquatic Organisms, 49(1), 33-38.
Aguayo, Juan ; Miquel, Alvaro ; Aranki, Naddim ; Jamett, Adolfo ; Valenzuela, Pablo D.T. ; Burzio, Luis O. / Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies. En: Diseases of Aquatic Organisms. 2002 ; Vol. 49, N.º 1. pp. 33-38.
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abstract = "An enzyme-linked immunosorbent assay (ELISA) for the detection of Piscirickettsia salmonis in fish tissue samples was developed. The test uses a combination of different monoclonal antibodies specific against P. salmonis in the capture step of the assay. The antibodies 7G4, 6E2 and 2C1 chosen for the capture step are bound to the solid support with an adhesive protein purified from a bivalve mollusc, resulting in a high yield of adsorption and binding stability. The monoclonal antibody 7G4, used as a second antibody, is conjugated to horseradish peroxidase. The resulting ELISA test detected 7 different isolates of P. salmonis and does not cross-react with several other fish pathogens, revealing a high specificity and sensitivity. The test also detects P. salmonis in kidney tissue of infected coho salmon with 98{\%} correlation with the immunofluorescence assay.",
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Aguayo, J, Miquel, A, Aranki, N, Jamett, A, Valenzuela, PDT & Burzio, LO 2002, 'Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies', Diseases of Aquatic Organisms, vol. 49, n.º 1, pp. 33-38.

Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies. / Aguayo, Juan; Miquel, Alvaro; Aranki, Naddim; Jamett, Adolfo; Valenzuela, Pablo D.T.; Burzio, Luis O.

En: Diseases of Aquatic Organisms, Vol. 49, N.º 1, 24.04.2002, p. 33-38.

Resultado de la investigación: Article

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T1 - Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies

AU - Aguayo, Juan

AU - Miquel, Alvaro

AU - Aranki, Naddim

AU - Jamett, Adolfo

AU - Valenzuela, Pablo D.T.

AU - Burzio, Luis O.

PY - 2002/4/24

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N2 - An enzyme-linked immunosorbent assay (ELISA) for the detection of Piscirickettsia salmonis in fish tissue samples was developed. The test uses a combination of different monoclonal antibodies specific against P. salmonis in the capture step of the assay. The antibodies 7G4, 6E2 and 2C1 chosen for the capture step are bound to the solid support with an adhesive protein purified from a bivalve mollusc, resulting in a high yield of adsorption and binding stability. The monoclonal antibody 7G4, used as a second antibody, is conjugated to horseradish peroxidase. The resulting ELISA test detected 7 different isolates of P. salmonis and does not cross-react with several other fish pathogens, revealing a high specificity and sensitivity. The test also detects P. salmonis in kidney tissue of infected coho salmon with 98% correlation with the immunofluorescence assay.

AB - An enzyme-linked immunosorbent assay (ELISA) for the detection of Piscirickettsia salmonis in fish tissue samples was developed. The test uses a combination of different monoclonal antibodies specific against P. salmonis in the capture step of the assay. The antibodies 7G4, 6E2 and 2C1 chosen for the capture step are bound to the solid support with an adhesive protein purified from a bivalve mollusc, resulting in a high yield of adsorption and binding stability. The monoclonal antibody 7G4, used as a second antibody, is conjugated to horseradish peroxidase. The resulting ELISA test detected 7 different isolates of P. salmonis and does not cross-react with several other fish pathogens, revealing a high specificity and sensitivity. The test also detects P. salmonis in kidney tissue of infected coho salmon with 98% correlation with the immunofluorescence assay.

KW - ELISA

KW - Monoclonal antibodies

KW - Piscirickettsia salmonis

KW - Piscirickettsiosis

KW - Salmon diseases

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JF - Diseases of Aquatic Organisms

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