Detection of evolutionarily distinct avian influenza a viruses in Antarctica

Aeron C. Hurt, Dhanasekaran Vijaykrishna, Jeffrey Butler, Chantal Baas, Sebastian Maurer-Stroh, M. Carolina Silva-de-la-Fuente, Gonzalo Medina-Vogel, Bjorn Olsen, Anne Kelso, Ian G. Barr, Daniel González-Acuña

Resultado de la investigación: Article

31 Citas (Scopus)

Resumen

Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adélie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra- and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment.

Idioma originalEnglish
PublicaciónmBio
Volumen5
N.º3
DOI
EstadoPublished - 6 may 2014

Huella dactilar

Influenza in Birds
Orthomyxoviridae
Genes
H3N8 Subtype Influenza A Virus
Spheniscidae
Viruses
Ferrets
Neuraminidase
Serology
Phylogeny
Ecology
Human Influenza
Horses
Birds
Genome

ASJC Scopus subject areas

  • Microbiology
  • Virology

Citar esto

Hurt, A. C., Vijaykrishna, D., Butler, J., Baas, C., Maurer-Stroh, S., Carolina Silva-de-la-Fuente, M., ... González-Acuña, D. (2014). Detection of evolutionarily distinct avian influenza a viruses in Antarctica. mBio, 5(3). https://doi.org/10.1128/mBio.01098-14
Hurt, Aeron C. ; Vijaykrishna, Dhanasekaran ; Butler, Jeffrey ; Baas, Chantal ; Maurer-Stroh, Sebastian ; Carolina Silva-de-la-Fuente, M. ; Medina-Vogel, Gonzalo ; Olsen, Bjorn ; Kelso, Anne ; Barr, Ian G. ; González-Acuña, Daniel. / Detection of evolutionarily distinct avian influenza a viruses in Antarctica. En: mBio. 2014 ; Vol. 5, N.º 3.
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abstract = "Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Ad{\'e}lie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra- and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment.",
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Hurt, AC, Vijaykrishna, D, Butler, J, Baas, C, Maurer-Stroh, S, Carolina Silva-de-la-Fuente, M, Medina-Vogel, G, Olsen, B, Kelso, A, Barr, IG & González-Acuña, D 2014, 'Detection of evolutionarily distinct avian influenza a viruses in Antarctica', mBio, vol. 5, n.º 3. https://doi.org/10.1128/mBio.01098-14

Detection of evolutionarily distinct avian influenza a viruses in Antarctica. / Hurt, Aeron C.; Vijaykrishna, Dhanasekaran; Butler, Jeffrey; Baas, Chantal; Maurer-Stroh, Sebastian; Carolina Silva-de-la-Fuente, M.; Medina-Vogel, Gonzalo; Olsen, Bjorn; Kelso, Anne; Barr, Ian G.; González-Acuña, Daniel.

En: mBio, Vol. 5, N.º 3, 06.05.2014.

Resultado de la investigación: Article

TY - JOUR

T1 - Detection of evolutionarily distinct avian influenza a viruses in Antarctica

AU - Hurt, Aeron C.

AU - Vijaykrishna, Dhanasekaran

AU - Butler, Jeffrey

AU - Baas, Chantal

AU - Maurer-Stroh, Sebastian

AU - Carolina Silva-de-la-Fuente, M.

AU - Medina-Vogel, Gonzalo

AU - Olsen, Bjorn

AU - Kelso, Anne

AU - Barr, Ian G.

AU - González-Acuña, Daniel

PY - 2014/5/6

Y1 - 2014/5/6

N2 - Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adélie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra- and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment.

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Hurt AC, Vijaykrishna D, Butler J, Baas C, Maurer-Stroh S, Carolina Silva-de-la-Fuente M y otros. Detection of evolutionarily distinct avian influenza a viruses in Antarctica. mBio. 2014 may 6;5(3). https://doi.org/10.1128/mBio.01098-14