Detection of antimicrobial residues in poultry litter: Monitoring a risk through a selective and sensitive hplc–ms/ms method

Karina Yévenes, Ekaterina Pokrant, Lina Trincado, Lisette Lapierre, Nicolás Galarce, Betty San Martín, Aldo Maddaleno, Héctor Hidalgo, Javiera Cornejo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread AMs residues in the environment. The aim of this research was to develop a method that could simultaneously identify and quantify three AMs families in poultry litter by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Samples of AMs free poultry litter were used to validate the method according to 657/2002/EC and VICH GL49. Results indicate that limit of detection (LOD) ranged from 8.95 to 20.86 µg kg−1, while limits of quantitation (LOQ) values were between 26.85 and 62.58 µg kg−1 of tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, enrofloxacin, ciprofloxacin, flumequine, sulfachloropyridazine, and sulfadiazine. Recoveries obtained ranged from 93 to 108%. The analysis of field samples obtained from seven commercial poultry flocks confirmed the adequacy of the method since it detected means concentrations ranging from 20 to 10,364 µg kg−1 . This provides us an accurate and reliable tool to monitor AMs residues in poultry litter and control its use as agricultural fertilizer.

Idioma originalInglés
Número de artículo1399
PublicaciónAnimals
Volumen11
N.º5
DOI
EstadoPublicada - may. 2021
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Animales y zoología
  • Veterinaria (todo)

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