Dendritic Cells and B Cells Cooperate in the Generation of CD4+CD25+FOXP3+ Allogeneic T Cells

C. Moore, D. Sauma, P. A. Reyes, J. Morales, M. Rosemblatt, M. R. Bono, J. A. Fierro

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Background: CD4+CD25+Foxp3+ regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF-β) is able to differentiate naïve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF-β. Methods: Splenic CD4+CD25- naïve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF-β, RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs. Results: Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF-β, RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival.

Idioma originalEnglish
Páginas (desde-hasta)371-375
Número de páginas5
PublicaciónTransplantation Proceedings
Volumen42
N.º1
DOI
EstadoPublished - ene 2010

Huella dactilar

Transforming Growth Factors
Antigen-Presenting Cells
Tretinoin
Dendritic Cells
Interleukin-2
B-Lymphocytes
T-Lymphocytes
Regulatory T-Lymphocytes
CD11c Antigens
Immune Tolerance
Isoantigens
Autoantigens
Coculture Techniques
Inbred C57BL Mouse
Immunosuppression
Cell Differentiation
Cell Survival
Flow Cytometry
Cell Culture Techniques
Antigens

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Citar esto

Moore, C. ; Sauma, D. ; Reyes, P. A. ; Morales, J. ; Rosemblatt, M. ; Bono, M. R. ; Fierro, J. A. / Dendritic Cells and B Cells Cooperate in the Generation of CD4+CD25+FOXP3+ Allogeneic T Cells. En: Transplantation Proceedings. 2010 ; Vol. 42, N.º 1. pp. 371-375.
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title = "Dendritic Cells and B Cells Cooperate in the Generation of CD4+CD25+FOXP3+ Allogeneic T Cells",
abstract = "Background: CD4+CD25+Foxp3+ regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF-β) is able to differentiate na{\"i}ve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF-β. Methods: Splenic CD4+CD25- na{\"i}ve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF-β, RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs. Results: Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF-β, RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival.",
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Dendritic Cells and B Cells Cooperate in the Generation of CD4+CD25+FOXP3+ Allogeneic T Cells. / Moore, C.; Sauma, D.; Reyes, P. A.; Morales, J.; Rosemblatt, M.; Bono, M. R.; Fierro, J. A.

En: Transplantation Proceedings, Vol. 42, N.º 1, 01.2010, p. 371-375.

Resultado de la investigación: Article

TY - JOUR

T1 - Dendritic Cells and B Cells Cooperate in the Generation of CD4+CD25+FOXP3+ Allogeneic T Cells

AU - Moore, C.

AU - Sauma, D.

AU - Reyes, P. A.

AU - Morales, J.

AU - Rosemblatt, M.

AU - Bono, M. R.

AU - Fierro, J. A.

PY - 2010/1

Y1 - 2010/1

N2 - Background: CD4+CD25+Foxp3+ regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF-β) is able to differentiate naïve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF-β. Methods: Splenic CD4+CD25- naïve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF-β, RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs. Results: Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF-β, RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival.

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