Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium

Steffen Porwollik, Carlos A. Santiviago, Pui Cheng, Fred Long, Prerak Desai, Jennifer Fredlund, Shabarinath Srikumar, Cecilia A. Silva, Weiping Chu, Xin Chen, Rocío Canals, M. Megan Reynolds, Lydia Bogomolnaya, Christine Shields, Ping Cui, Jinbai Guo, Yi Zheng, Tiana Endicott-Yazdani, Hee Jeong Yang, Aimee MapleYury Ragoza, Carlos J. Blondel, Camila Valenzuela, Helene Andrews-Polymenis, Michael McClelland

Resultado de la investigación: Article

47 Citas (Scopus)

Resumen

We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.

Idioma originalEnglish
Número de artículoe99820
PublicaciónPLoS ONE
Volumen9
N.º7
DOI
EstadoPublished - 9 jul 2014

Huella dactilar

Salmonella
Salmonella enterica
gene deletion
Gene Deletion
Genes
mutants
genes
Kanamycin Resistance
kanamycin
Chloramphenicol Resistance
Kanamycin
screening
chloramphenicol
Screening
Bearings (structural)
Libraries
antibiotics
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Citar esto

Porwollik, S., Santiviago, C. A., Cheng, P., Long, F., Desai, P., Fredlund, J., ... McClelland, M. (2014). Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium. PLoS ONE, 9(7), [e99820]. https://doi.org/10.1371/journal.pone.0099820
Porwollik, Steffen ; Santiviago, Carlos A. ; Cheng, Pui ; Long, Fred ; Desai, Prerak ; Fredlund, Jennifer ; Srikumar, Shabarinath ; Silva, Cecilia A. ; Chu, Weiping ; Chen, Xin ; Canals, Rocío ; Reynolds, M. Megan ; Bogomolnaya, Lydia ; Shields, Christine ; Cui, Ping ; Guo, Jinbai ; Zheng, Yi ; Endicott-Yazdani, Tiana ; Yang, Hee Jeong ; Maple, Aimee ; Ragoza, Yury ; Blondel, Carlos J. ; Valenzuela, Camila ; Andrews-Polymenis, Helene ; McClelland, Michael. / Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium. En: PLoS ONE. 2014 ; Vol. 9, N.º 7.
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title = "Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium",
abstract = "We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.",
author = "Steffen Porwollik and Santiviago, {Carlos A.} and Pui Cheng and Fred Long and Prerak Desai and Jennifer Fredlund and Shabarinath Srikumar and Silva, {Cecilia A.} and Weiping Chu and Xin Chen and Roc{\'i}o Canals and Reynolds, {M. Megan} and Lydia Bogomolnaya and Christine Shields and Ping Cui and Jinbai Guo and Yi Zheng and Tiana Endicott-Yazdani and Yang, {Hee Jeong} and Aimee Maple and Yury Ragoza and Blondel, {Carlos J.} and Camila Valenzuela and Helene Andrews-Polymenis and Michael McClelland",
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Porwollik, S, Santiviago, CA, Cheng, P, Long, F, Desai, P, Fredlund, J, Srikumar, S, Silva, CA, Chu, W, Chen, X, Canals, R, Reynolds, MM, Bogomolnaya, L, Shields, C, Cui, P, Guo, J, Zheng, Y, Endicott-Yazdani, T, Yang, HJ, Maple, A, Ragoza, Y, Blondel, CJ, Valenzuela, C, Andrews-Polymenis, H & McClelland, M 2014, 'Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium', PLoS ONE, vol. 9, n.º 7, e99820. https://doi.org/10.1371/journal.pone.0099820

Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium. / Porwollik, Steffen; Santiviago, Carlos A.; Cheng, Pui; Long, Fred; Desai, Prerak; Fredlund, Jennifer; Srikumar, Shabarinath; Silva, Cecilia A.; Chu, Weiping; Chen, Xin; Canals, Rocío; Reynolds, M. Megan; Bogomolnaya, Lydia; Shields, Christine; Cui, Ping; Guo, Jinbai; Zheng, Yi; Endicott-Yazdani, Tiana; Yang, Hee Jeong; Maple, Aimee; Ragoza, Yury; Blondel, Carlos J.; Valenzuela, Camila; Andrews-Polymenis, Helene; McClelland, Michael.

En: PLoS ONE, Vol. 9, N.º 7, e99820, 09.07.2014.

Resultado de la investigación: Article

TY - JOUR

T1 - Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium

AU - Porwollik, Steffen

AU - Santiviago, Carlos A.

AU - Cheng, Pui

AU - Long, Fred

AU - Desai, Prerak

AU - Fredlund, Jennifer

AU - Srikumar, Shabarinath

AU - Silva, Cecilia A.

AU - Chu, Weiping

AU - Chen, Xin

AU - Canals, Rocío

AU - Reynolds, M. Megan

AU - Bogomolnaya, Lydia

AU - Shields, Christine

AU - Cui, Ping

AU - Guo, Jinbai

AU - Zheng, Yi

AU - Endicott-Yazdani, Tiana

AU - Yang, Hee Jeong

AU - Maple, Aimee

AU - Ragoza, Yury

AU - Blondel, Carlos J.

AU - Valenzuela, Camila

AU - Andrews-Polymenis, Helene

AU - McClelland, Michael

PY - 2014/7/9

Y1 - 2014/7/9

N2 - We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.

AB - We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.

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Porwollik S, Santiviago CA, Cheng P, Long F, Desai P, Fredlund J y otros. Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium. PLoS ONE. 2014 jul 9;9(7). e99820. https://doi.org/10.1371/journal.pone.0099820