Defense mechanism of citrus limon seedlings against fungal pathogens: signal transduction and pal gene

D. Seelenfreund, P. Castafieda, S. Lobes, X. P. Ortega, R. Polanco, M. Quiroz, L. M. Pdrez

Resultado de la investigación: Article

Resumen

Citrus limon seedlings respond to fungat attack inducing the phenylpropanoid pathway producing phytoalexins. The de nero synthesis of one of the main enzymes, phenylalanine ammonia-lyase (PAL), is accomplished after 24 hours inoculation with Alternaria aIternata, or after 3 - 4 hours in response to elicitors. The transduction of these signals requires Ca2+ active calcium ion channels. We present evidence of other components of the signal transduction pathway, as well as characteristics of the PAL gene. The participation of calmodulin, PK and phosphoinositides was analyzed using specific antagonists or inhibitors, before eliciting lemon seedlings. Trifluoperazine and W-7 (CaM antagonists), staurosporine (PKC inhibitor), 2,5-dihydroxyeinnamate methyl ester and lavendustin A (PTK inhibitors) and caffeine (IP3 receptor antagonist) prevented the induction of PAL. The corresponding ICs0 values are similar to those observed in animal systems. A PAL gene was isolated using PCR primers based on a cDNA sequence from C. limon (access number U43338), which contains a single intron of 1.678 kb, splitting the codifying sequence into exon 1 (N-terminal) of 413 bp and exon 2 (C-terminal) of 1,753 bp, the size of the gene being 3.84 kb. FONDECYT 1,940441-1970532.

Idioma originalEnglish
PublicaciónFASEB Journal
Volumen11
N.º9
EstadoPublished - 1997

Huella dactilar

Phenylalanine Ammonia-Lyase
Citrus limon
Signal transduction
Citrus
phenylalanine ammonia-lyase
Pathogens
Seedlings
defense mechanisms
signal transduction
Signal Transduction
Genes
antagonists
seedlings
pathogens
exons
Exons
genes
trifluoperazine
Inositol 1,4,5-Trisphosphate Receptors
calcium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Biotechnology

Citar esto

Seelenfreund, D., Castafieda, P., Lobes, S., Ortega, X. P., Polanco, R., Quiroz, M., & Pdrez, L. M. (1997). Defense mechanism of citrus limon seedlings against fungal pathogens: signal transduction and pal gene. FASEB Journal, 11(9).
Seelenfreund, D. ; Castafieda, P. ; Lobes, S. ; Ortega, X. P. ; Polanco, R. ; Quiroz, M. ; Pdrez, L. M. / Defense mechanism of citrus limon seedlings against fungal pathogens : signal transduction and pal gene. En: FASEB Journal. 1997 ; Vol. 11, N.º 9.
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Seelenfreund, D, Castafieda, P, Lobes, S, Ortega, XP, Polanco, R, Quiroz, M & Pdrez, LM 1997, 'Defense mechanism of citrus limon seedlings against fungal pathogens: signal transduction and pal gene', FASEB Journal, vol. 11, n.º 9.

Defense mechanism of citrus limon seedlings against fungal pathogens : signal transduction and pal gene. / Seelenfreund, D.; Castafieda, P.; Lobes, S.; Ortega, X. P.; Polanco, R.; Quiroz, M.; Pdrez, L. M.

En: FASEB Journal, Vol. 11, N.º 9, 1997.

Resultado de la investigación: Article

TY - JOUR

T1 - Defense mechanism of citrus limon seedlings against fungal pathogens

T2 - signal transduction and pal gene

AU - Seelenfreund, D.

AU - Castafieda, P.

AU - Lobes, S.

AU - Ortega, X. P.

AU - Polanco, R.

AU - Quiroz, M.

AU - Pdrez, L. M.

PY - 1997

Y1 - 1997

N2 - Citrus limon seedlings respond to fungat attack inducing the phenylpropanoid pathway producing phytoalexins. The de nero synthesis of one of the main enzymes, phenylalanine ammonia-lyase (PAL), is accomplished after 24 hours inoculation with Alternaria aIternata, or after 3 - 4 hours in response to elicitors. The transduction of these signals requires Ca2+ active calcium ion channels. We present evidence of other components of the signal transduction pathway, as well as characteristics of the PAL gene. The participation of calmodulin, PK and phosphoinositides was analyzed using specific antagonists or inhibitors, before eliciting lemon seedlings. Trifluoperazine and W-7 (CaM antagonists), staurosporine (PKC inhibitor), 2,5-dihydroxyeinnamate methyl ester and lavendustin A (PTK inhibitors) and caffeine (IP3 receptor antagonist) prevented the induction of PAL. The corresponding ICs0 values are similar to those observed in animal systems. A PAL gene was isolated using PCR primers based on a cDNA sequence from C. limon (access number U43338), which contains a single intron of 1.678 kb, splitting the codifying sequence into exon 1 (N-terminal) of 413 bp and exon 2 (C-terminal) of 1,753 bp, the size of the gene being 3.84 kb. FONDECYT 1,940441-1970532.

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