Cytotoxicity and in vivo plasma kinetic behavior of surface-functionalized PAMAM dendrimers

Carola Diaz, Carolina Benitez, Felipe Vidal, Luis F. Barraza, Verónica A. Jiménez, Leonardo Guzman, Jorge Fuentealba, Gonzalo E. Yevenes, Joel B. Alderete

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

18 Citas (Scopus)

Resumen

Understanding the molecular features responsible for the plasma kinetics of surface-modified polyamido amine (PAMAM) dendrimers is critical to explore novel biomedical applications for these nanomaterials. In this report, polyethylene glycol (PEG) and folic acid (FA) were employed to obtain partially-substituted PAMAM dendrimers as model biocompatible nanomaterials with different size, charge and surface functionality. Cytotoxicity assays on HEK cells at 1-500 μM concentration confirmed that PEG and FA incorporation increased the cell viability of PAMAM-based nanomaterials. Measurements of plasma kinetics in vivo revealed that PEG-PAMAM has an extended circulation time in mice blood (71.7 min) over native PAMAM (53.3 min) and FA-PAMAM (41.8 min). Molecular dynamics simulations revealed a direct relationship between circulation time and dendrimer size, thus providing valuable evidence to increase understanding about the modulation of functional properties of PAMAM-based systems through surface modification, and to guide future efforts on the rational design of novel biomedical nanomaterials.

Idioma originalInglés
Páginas (desde-hasta)2227-2234
Número de páginas8
PublicaciónNanomedicine: Nanotechnology, Biology, and Medicine
Volumen14
N.º7
DOI
EstadoPublicada - 1 oct 2018

Áreas temáticas de ASJC Scopus

  • Bioingeniería
  • Medicina (miscelánea)
  • Medicina molecular
  • Ingeniería biomédica
  • Ciencia de los materiales (todo)
  • Ciencias farmacéuticas

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