Cytoplasm of sea urchin unfertilized eggs contains a nucleosome remodeling activity

Ricardo Medina, José Gutiérrez, Marcia Puchi, María Imschenetzky, Martin Montecino

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

After fertilization the sea urchin sperm nucleus transforms into the male pronucleus which later fuses with the female pronucleus re-establishing the diploid genome of the embryo. This process requires remodeling of the sperm chromatin structure including the replacement of the sperm histones by maternally derived cleavage stage histone variants. In recent years, a group of protein complexes that promote chromatin-remodeling in an ATP-dependent manner have been described. To gain understanding into the molecular mechanisms operating during sea urchin male pronuclei formation, we analyzed whether chromatin-remodeling activity was present in unfertilized eggs as well as during early embryogenesis. We report that in the sea urchin Tetrapygus niger, protein extracts from the cytoplasm but not from the nucleus, of unfertilized eggs exhibit ATP-dependent nucleosome remodeling activity. This cytosolic activity was not found at early stages of sea urchin embryogenesis. In addition, by using polyclonal antibodies in Western blot analyses, we found that an ISWI-related protein is primarily localized in the cytoplasm of the sea urchin eggs. Interestingly, SWI2/SNF2-related proteins were not detected neither in the nucleus nor in the cytoplasm of unfertilized eggs. During embryogenesis, as transcriptional activity is increased an ISWI-related protein is found principally in the nuclear fraction. Together, our results indicate that the cytoplasm in sea urchin eggs contains an ATP-dependent chromatin-remodeling activity, which may include ISWI as a catalytic subunit.

Idioma originalEnglish
Páginas (desde-hasta)554-562
Número de páginas9
PublicaciónJournal of Cellular Biochemistry
Volumen83
N.º4
DOI
EstadoPublished - 2001

Huella dactilar

Sea Urchins
Nucleosomes
Ovum
Cytoplasm
Chromatin Assembly and Disassembly
Chromatin
Adenosine Triphosphate
Embryonic Development
Spermatozoa
Proteins
Histones
Eggs
Electric fuses
Diploidy
Fertilization
Genes
Catalytic Domain
Embryonic Structures
Western Blotting
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

Medina, Ricardo ; Gutiérrez, José ; Puchi, Marcia ; Imschenetzky, María ; Montecino, Martin. / Cytoplasm of sea urchin unfertilized eggs contains a nucleosome remodeling activity. En: Journal of Cellular Biochemistry. 2001 ; Vol. 83, N.º 4. pp. 554-562.
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abstract = "After fertilization the sea urchin sperm nucleus transforms into the male pronucleus which later fuses with the female pronucleus re-establishing the diploid genome of the embryo. This process requires remodeling of the sperm chromatin structure including the replacement of the sperm histones by maternally derived cleavage stage histone variants. In recent years, a group of protein complexes that promote chromatin-remodeling in an ATP-dependent manner have been described. To gain understanding into the molecular mechanisms operating during sea urchin male pronuclei formation, we analyzed whether chromatin-remodeling activity was present in unfertilized eggs as well as during early embryogenesis. We report that in the sea urchin Tetrapygus niger, protein extracts from the cytoplasm but not from the nucleus, of unfertilized eggs exhibit ATP-dependent nucleosome remodeling activity. This cytosolic activity was not found at early stages of sea urchin embryogenesis. In addition, by using polyclonal antibodies in Western blot analyses, we found that an ISWI-related protein is primarily localized in the cytoplasm of the sea urchin eggs. Interestingly, SWI2/SNF2-related proteins were not detected neither in the nucleus nor in the cytoplasm of unfertilized eggs. During embryogenesis, as transcriptional activity is increased an ISWI-related protein is found principally in the nuclear fraction. Together, our results indicate that the cytoplasm in sea urchin eggs contains an ATP-dependent chromatin-remodeling activity, which may include ISWI as a catalytic subunit.",
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Cytoplasm of sea urchin unfertilized eggs contains a nucleosome remodeling activity. / Medina, Ricardo; Gutiérrez, José; Puchi, Marcia; Imschenetzky, María; Montecino, Martin.

En: Journal of Cellular Biochemistry, Vol. 83, N.º 4, 2001, p. 554-562.

Resultado de la investigación: Article

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T1 - Cytoplasm of sea urchin unfertilized eggs contains a nucleosome remodeling activity

AU - Medina, Ricardo

AU - Gutiérrez, José

AU - Puchi, Marcia

AU - Imschenetzky, María

AU - Montecino, Martin

PY - 2001

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AB - After fertilization the sea urchin sperm nucleus transforms into the male pronucleus which later fuses with the female pronucleus re-establishing the diploid genome of the embryo. This process requires remodeling of the sperm chromatin structure including the replacement of the sperm histones by maternally derived cleavage stage histone variants. In recent years, a group of protein complexes that promote chromatin-remodeling in an ATP-dependent manner have been described. To gain understanding into the molecular mechanisms operating during sea urchin male pronuclei formation, we analyzed whether chromatin-remodeling activity was present in unfertilized eggs as well as during early embryogenesis. We report that in the sea urchin Tetrapygus niger, protein extracts from the cytoplasm but not from the nucleus, of unfertilized eggs exhibit ATP-dependent nucleosome remodeling activity. This cytosolic activity was not found at early stages of sea urchin embryogenesis. In addition, by using polyclonal antibodies in Western blot analyses, we found that an ISWI-related protein is primarily localized in the cytoplasm of the sea urchin eggs. Interestingly, SWI2/SNF2-related proteins were not detected neither in the nucleus nor in the cytoplasm of unfertilized eggs. During embryogenesis, as transcriptional activity is increased an ISWI-related protein is found principally in the nuclear fraction. Together, our results indicate that the cytoplasm in sea urchin eggs contains an ATP-dependent chromatin-remodeling activity, which may include ISWI as a catalytic subunit.

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