Cyclosporin a-treated dendritic cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory t cell proliferation

Karina Pino-Lagos, Paula Michea, Daniela Sauma, Andrea Alba, Jorge Morales, María Rosa Bono, Alberto Fierro, Mario Rosemblatt

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (TREG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect TREG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled TREG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and increased IL-10 secretion when stimulated with LPS, indicating an effect on the polarization of the immune response. Interestingly, CsA-treated DCs show an anti-tolerogenic effect since they reduce the proliferation of TREG cells from 72 to 47%. Further inhibition to a 24% of TREG proliferation was obtained as a direct effect of CsA on TREG. In conclusion, the anti-tolerogenic effect of CsA should be considered in the planning of immunosuppression in the context of clinical transplantation.

Idioma originalEnglish
Páginas (desde-hasta)333-337
Número de páginas5
PublicaciónBiological Research
Volumen43
N.º3
DOI
EstadoPublished - 1 ene 2010

Huella dactilar

cyclosporins
Transplantation (surgical)
organ transplantation
cyclosporine
Cell proliferation
Organ Transplantation
dendritic cells
Dendritic Cells
Cyclosporine
cell proliferation
Cell Proliferation
Phenotype
phenotype
immunosuppressive agents
T-cells
Flow cytometry
interleukin-12
immunosuppression
mice
Interleukin-12

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Pino-Lagos, Karina ; Michea, Paula ; Sauma, Daniela ; Alba, Andrea ; Morales, Jorge ; Bono, María Rosa ; Fierro, Alberto ; Rosemblatt, Mario. / Cyclosporin a-treated dendritic cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory t cell proliferation. En: Biological Research. 2010 ; Vol. 43, N.º 3. pp. 333-337.
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abstract = "One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (TREG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect TREG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled TREG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and increased IL-10 secretion when stimulated with LPS, indicating an effect on the polarization of the immune response. Interestingly, CsA-treated DCs show an anti-tolerogenic effect since they reduce the proliferation of TREG cells from 72 to 47{\%}. Further inhibition to a 24{\%} of TREG proliferation was obtained as a direct effect of CsA on TREG. In conclusion, the anti-tolerogenic effect of CsA should be considered in the planning of immunosuppression in the context of clinical transplantation.",
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Cyclosporin a-treated dendritic cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory t cell proliferation. / Pino-Lagos, Karina; Michea, Paula; Sauma, Daniela; Alba, Andrea; Morales, Jorge; Bono, María Rosa; Fierro, Alberto; Rosemblatt, Mario.

En: Biological Research, Vol. 43, N.º 3, 01.01.2010, p. 333-337.

Resultado de la investigación: Article

TY - JOUR

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AU - Pino-Lagos, Karina

AU - Michea, Paula

AU - Sauma, Daniela

AU - Alba, Andrea

AU - Morales, Jorge

AU - Bono, María Rosa

AU - Fierro, Alberto

AU - Rosemblatt, Mario

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