Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit

Nicolás Franck, Denisse Zamorano, Britt Wallberg, Charlotte Hardy, Mario Ahumada, Natalia Rivera, María Montoya, Claudio Urra, Claudio Meneses, Iván Balic, Nilo Mejía, Antonio Ibacache, Andrés Zurita-Silva

Resultado de la investigación: Article

Resumen

Viticulture is facing water deficit problems related to climate change, new extremes in heat and precipitation regimes and drought events. Rootstocks use was assessed as strategy for enhancing performance of Cabernet Sauvignon (CS) and Syrah (Sy) under water deficit. Vines were grafted onto naturalized grapevines selected from hyper-arid Chile, and compared to own-grafted and commercial Ruggeri 140. Plants were submitted to optimal (100 % ETc) and deficit (30 % ETc) irrigation throughout two seasons at field conditions. Functional traits along both seasons were determined. Water deficit reduced all growth and physiological traits especially in CS. R32 rootstock induced significantly higher values for most traits irrespective of cv and seasons associated to higher root growth. Transcriptomic analysis was further performed in both cultivars grafted over R32 rootstock by RNA-Seq, determining that gene up-regulation extent was higher in Sy. More stable transcriptional landscape was determined in CS than Sy, which might be linked to its hydric strategy. Unexpectedly, major differences in transcriptional behaviour were detected in R32 rootstock, revealing major transcriptional changes occurring at root level, suggesting scion-driven transcriptional regulation in response to stress. Finally, R32 rootstock can be considered for both near iso and anisohydric grapevines as adaptive strategy for climate constrains.

Idioma originalEnglish
Número de artículo109031
PublicaciónScientia Horticulturae
Volumen262
DOI
EstadoPublished - 27 feb 2020

Huella dactilar

scions
transcriptomics
rootstocks
water
viticulture
vines
Chile
stress response
root growth
drought
climate change
irrigation
RNA
climate
heat
cultivars
genes

ASJC Scopus subject areas

  • Horticulture

Citar esto

Franck, Nicolás ; Zamorano, Denisse ; Wallberg, Britt ; Hardy, Charlotte ; Ahumada, Mario ; Rivera, Natalia ; Montoya, María ; Urra, Claudio ; Meneses, Claudio ; Balic, Iván ; Mejía, Nilo ; Ibacache, Antonio ; Zurita-Silva, Andrés. / Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit. En: Scientia Horticulturae. 2020 ; Vol. 262.
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title = "Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit",
abstract = "Viticulture is facing water deficit problems related to climate change, new extremes in heat and precipitation regimes and drought events. Rootstocks use was assessed as strategy for enhancing performance of Cabernet Sauvignon (CS) and Syrah (Sy) under water deficit. Vines were grafted onto naturalized grapevines selected from hyper-arid Chile, and compared to own-grafted and commercial Ruggeri 140. Plants were submitted to optimal (100 {\%} ETc) and deficit (30 {\%} ETc) irrigation throughout two seasons at field conditions. Functional traits along both seasons were determined. Water deficit reduced all growth and physiological traits especially in CS. R32 rootstock induced significantly higher values for most traits irrespective of cv and seasons associated to higher root growth. Transcriptomic analysis was further performed in both cultivars grafted over R32 rootstock by RNA-Seq, determining that gene up-regulation extent was higher in Sy. More stable transcriptional landscape was determined in CS than Sy, which might be linked to its hydric strategy. Unexpectedly, major differences in transcriptional behaviour were detected in R32 rootstock, revealing major transcriptional changes occurring at root level, suggesting scion-driven transcriptional regulation in response to stress. Finally, R32 rootstock can be considered for both near iso and anisohydric grapevines as adaptive strategy for climate constrains.",
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Franck, N, Zamorano, D, Wallberg, B, Hardy, C, Ahumada, M, Rivera, N, Montoya, M, Urra, C, Meneses, C, Balic, I, Mejía, N, Ibacache, A & Zurita-Silva, A 2020, 'Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit', Scientia Horticulturae, vol. 262, 109031. https://doi.org/10.1016/j.scienta.2019.109031

Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit. / Franck, Nicolás; Zamorano, Denisse; Wallberg, Britt; Hardy, Charlotte; Ahumada, Mario; Rivera, Natalia; Montoya, María; Urra, Claudio; Meneses, Claudio; Balic, Iván; Mejía, Nilo; Ibacache, Antonio; Zurita-Silva, Andrés.

En: Scientia Horticulturae, Vol. 262, 109031, 27.02.2020.

Resultado de la investigación: Article

TY - JOUR

T1 - Contrasting grapevines grafted into naturalized rootstock suggest scion-driven transcriptomic changes in response to water deficit

AU - Franck, Nicolás

AU - Zamorano, Denisse

AU - Wallberg, Britt

AU - Hardy, Charlotte

AU - Ahumada, Mario

AU - Rivera, Natalia

AU - Montoya, María

AU - Urra, Claudio

AU - Meneses, Claudio

AU - Balic, Iván

AU - Mejía, Nilo

AU - Ibacache, Antonio

AU - Zurita-Silva, Andrés

PY - 2020/2/27

Y1 - 2020/2/27

N2 - Viticulture is facing water deficit problems related to climate change, new extremes in heat and precipitation regimes and drought events. Rootstocks use was assessed as strategy for enhancing performance of Cabernet Sauvignon (CS) and Syrah (Sy) under water deficit. Vines were grafted onto naturalized grapevines selected from hyper-arid Chile, and compared to own-grafted and commercial Ruggeri 140. Plants were submitted to optimal (100 % ETc) and deficit (30 % ETc) irrigation throughout two seasons at field conditions. Functional traits along both seasons were determined. Water deficit reduced all growth and physiological traits especially in CS. R32 rootstock induced significantly higher values for most traits irrespective of cv and seasons associated to higher root growth. Transcriptomic analysis was further performed in both cultivars grafted over R32 rootstock by RNA-Seq, determining that gene up-regulation extent was higher in Sy. More stable transcriptional landscape was determined in CS than Sy, which might be linked to its hydric strategy. Unexpectedly, major differences in transcriptional behaviour were detected in R32 rootstock, revealing major transcriptional changes occurring at root level, suggesting scion-driven transcriptional regulation in response to stress. Finally, R32 rootstock can be considered for both near iso and anisohydric grapevines as adaptive strategy for climate constrains.

AB - Viticulture is facing water deficit problems related to climate change, new extremes in heat and precipitation regimes and drought events. Rootstocks use was assessed as strategy for enhancing performance of Cabernet Sauvignon (CS) and Syrah (Sy) under water deficit. Vines were grafted onto naturalized grapevines selected from hyper-arid Chile, and compared to own-grafted and commercial Ruggeri 140. Plants were submitted to optimal (100 % ETc) and deficit (30 % ETc) irrigation throughout two seasons at field conditions. Functional traits along both seasons were determined. Water deficit reduced all growth and physiological traits especially in CS. R32 rootstock induced significantly higher values for most traits irrespective of cv and seasons associated to higher root growth. Transcriptomic analysis was further performed in both cultivars grafted over R32 rootstock by RNA-Seq, determining that gene up-regulation extent was higher in Sy. More stable transcriptional landscape was determined in CS than Sy, which might be linked to its hydric strategy. Unexpectedly, major differences in transcriptional behaviour were detected in R32 rootstock, revealing major transcriptional changes occurring at root level, suggesting scion-driven transcriptional regulation in response to stress. Finally, R32 rootstock can be considered for both near iso and anisohydric grapevines as adaptive strategy for climate constrains.

KW - Climate change

KW - Hydric strategy

KW - Rootstocks

KW - Transcriptomic

KW - Vitis vinifera

KW - Water deficit

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