CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS.

James R. Yates, David S. Holmes

Resultado de la investigación: Conference contribution

3 Citas (Scopus)

Resumen

There are special problems associated with the identification and quantitation of microorganisms in biohydrometallurgical operations. In almost every situation there are numerous species of autotrophic and heterotrophic bacteria. Many of these have similar morphologies and are difficult to purify since they grow poorly or not at all on solid media. In addition, analysis is frequently aggravated by the presence of small rock particles and by the production of ferric precipitates. Authors have developed a technique for the characterization and quantitation of bioleaching microorganisms that circumvents many of these problems. The technique utilizes molecular probes consisting of cloned DNA sequences. These probes can distinguish between species of Thiobacillus and can even differentiate between several strains of the single species Thiobacillus ferrooxidans. The probes are exquisitely sensitive permitting the detection of as few as 10**5 bacteria of a given species. Authors propose that the technique will be useful for the identification and quantitation of native microorganisms and also may be used to follow the growth of a laboratory strain or genetically-engineered microorganism in a bioleaching operation.

Idioma originalEnglish
Título de la publicación alojadaUnknown Host Publication Title
EditoresRichard W. Lawrence, Richard M.R. Branion, Hans G. Ebner
EditorialElsevier (Process Metallurgy 4)
Páginas409-418
Número de páginas10
ISBN (versión impresa)0444426582
EstadoPublished - 1986

Huella dactilar

Bioleaching
Microorganisms
Bacteria
DNA sequences
Precipitates
Rocks

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Yates, J. R., & Holmes, D. S. (1986). CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS. En R. W. Lawrence, R. M. R. Branion, & H. G. Ebner (Eds.), Unknown Host Publication Title (pp. 409-418). Elsevier (Process Metallurgy 4).
Yates, James R. ; Holmes, David S. / CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS. Unknown Host Publication Title. editor / Richard W. Lawrence ; Richard M.R. Branion ; Hans G. Ebner. Elsevier (Process Metallurgy 4), 1986. pp. 409-418
@inproceedings{f924e75476994a9bad351ca8e28fae43,
title = "CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS.",
abstract = "There are special problems associated with the identification and quantitation of microorganisms in biohydrometallurgical operations. In almost every situation there are numerous species of autotrophic and heterotrophic bacteria. Many of these have similar morphologies and are difficult to purify since they grow poorly or not at all on solid media. In addition, analysis is frequently aggravated by the presence of small rock particles and by the production of ferric precipitates. Authors have developed a technique for the characterization and quantitation of bioleaching microorganisms that circumvents many of these problems. The technique utilizes molecular probes consisting of cloned DNA sequences. These probes can distinguish between species of Thiobacillus and can even differentiate between several strains of the single species Thiobacillus ferrooxidans. The probes are exquisitely sensitive permitting the detection of as few as 10**5 bacteria of a given species. Authors propose that the technique will be useful for the identification and quantitation of native microorganisms and also may be used to follow the growth of a laboratory strain or genetically-engineered microorganism in a bioleaching operation.",
author = "Yates, {James R.} and Holmes, {David S.}",
year = "1986",
language = "English",
isbn = "0444426582",
pages = "409--418",
editor = "Lawrence, {Richard W.} and Branion, {Richard M.R.} and Ebner, {Hans G.}",
booktitle = "Unknown Host Publication Title",
publisher = "Elsevier (Process Metallurgy 4)",

}

Yates, JR & Holmes, DS 1986, CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS. En RW Lawrence, RMR Branion & HG Ebner (eds.), Unknown Host Publication Title. Elsevier (Process Metallurgy 4), pp. 409-418.

CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS. / Yates, James R.; Holmes, David S.

Unknown Host Publication Title. ed. / Richard W. Lawrence; Richard M.R. Branion; Hans G. Ebner. Elsevier (Process Metallurgy 4), 1986. p. 409-418.

Resultado de la investigación: Conference contribution

TY - GEN

T1 - CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS.

AU - Yates, James R.

AU - Holmes, David S.

PY - 1986

Y1 - 1986

N2 - There are special problems associated with the identification and quantitation of microorganisms in biohydrometallurgical operations. In almost every situation there are numerous species of autotrophic and heterotrophic bacteria. Many of these have similar morphologies and are difficult to purify since they grow poorly or not at all on solid media. In addition, analysis is frequently aggravated by the presence of small rock particles and by the production of ferric precipitates. Authors have developed a technique for the characterization and quantitation of bioleaching microorganisms that circumvents many of these problems. The technique utilizes molecular probes consisting of cloned DNA sequences. These probes can distinguish between species of Thiobacillus and can even differentiate between several strains of the single species Thiobacillus ferrooxidans. The probes are exquisitely sensitive permitting the detection of as few as 10**5 bacteria of a given species. Authors propose that the technique will be useful for the identification and quantitation of native microorganisms and also may be used to follow the growth of a laboratory strain or genetically-engineered microorganism in a bioleaching operation.

AB - There are special problems associated with the identification and quantitation of microorganisms in biohydrometallurgical operations. In almost every situation there are numerous species of autotrophic and heterotrophic bacteria. Many of these have similar morphologies and are difficult to purify since they grow poorly or not at all on solid media. In addition, analysis is frequently aggravated by the presence of small rock particles and by the production of ferric precipitates. Authors have developed a technique for the characterization and quantitation of bioleaching microorganisms that circumvents many of these problems. The technique utilizes molecular probes consisting of cloned DNA sequences. These probes can distinguish between species of Thiobacillus and can even differentiate between several strains of the single species Thiobacillus ferrooxidans. The probes are exquisitely sensitive permitting the detection of as few as 10**5 bacteria of a given species. Authors propose that the technique will be useful for the identification and quantitation of native microorganisms and also may be used to follow the growth of a laboratory strain or genetically-engineered microorganism in a bioleaching operation.

UR - http://www.scopus.com/inward/record.url?scp=0022946693&partnerID=8YFLogxK

M3 - Conference contribution

SN - 0444426582

SP - 409

EP - 418

BT - Unknown Host Publication Title

A2 - Lawrence, Richard W.

A2 - Branion, Richard M.R.

A2 - Ebner, Hans G.

PB - Elsevier (Process Metallurgy 4)

ER -

Yates JR, Holmes DS. CONSTRUCTION AND USE OF MOLECULAR PROBES TO IDENTIFY AND QUANTITATE BIOLEACHING MICROORGANISMS. En Lawrence RW, Branion RMR, Ebner HG, editores, Unknown Host Publication Title. Elsevier (Process Metallurgy 4). 1986. p. 409-418