Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars

Carolina Klagges, José Antonio Campoy, José Quero-García, Alejandra Guzmán, Levi Mansur, Eduardo Gratacós, Herman Silva, Umesh R. Rosyara, Amy Iezzoni, Lee A. Meisel, Elisabeth Dirlewanger

Resultado de la investigación: Contribución a la publicaciónArticle

  • 33 Citas

Resumen

Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs) provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L.) intra-specific progenies derived from crosses between 'Black Tartarian' × 'Kordia' (BT×K) and 'Regina' × 'Lapins'(R×L), high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F1 plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs) in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1-LG8). These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family.

IdiomaEnglish
Número de artículoe54743
PublicaciónPLoS ONE
Volumen8
Número de edición1
DOI
EstadoPublished - 31 ene 2013

Huella dactilar

Prunus avium
Synteny
peaches
chromosome mapping
Genome
genome
Prunus
cultivars
Genes
linkage groups
single nucleotide polymorphism
Single Nucleotide Polymorphism
Rosaceae
temperate fruits
Polymorphism
geneticists
Genetic Linkage
Chromosome Mapping
Malus
Nucleotides

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Klagges, C., Campoy, J. A., Quero-García, J., Guzmán, A., Mansur, L., Gratacós, E., ... Dirlewanger, E. (2013). Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars. PLoS ONE, 8(1), [e54743]. DOI: 10.1371/journal.pone.0054743
Klagges, Carolina ; Campoy, José Antonio ; Quero-García, José ; Guzmán, Alejandra ; Mansur, Levi ; Gratacós, Eduardo ; Silva, Herman ; Rosyara, Umesh R. ; Iezzoni, Amy ; Meisel, Lee A. ; Dirlewanger, Elisabeth. / Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars. En: PLoS ONE. 2013 ; Vol. 8, N.º 1.
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abstract = "Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs) provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L.) intra-specific progenies derived from crosses between 'Black Tartarian' × 'Kordia' (BT×K) and 'Regina' × 'Lapins'(R×L), high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F1 plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs) in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1-LG8). These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family.",
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Klagges, C, Campoy, JA, Quero-García, J, Guzmán, A, Mansur, L, Gratacós, E, Silva, H, Rosyara, UR, Iezzoni, A, Meisel, LA & Dirlewanger, E 2013, 'Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars' PLoS ONE, vol. 8, n.º 1, e54743. DOI: 10.1371/journal.pone.0054743

Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars. / Klagges, Carolina; Campoy, José Antonio; Quero-García, José; Guzmán, Alejandra; Mansur, Levi; Gratacós, Eduardo; Silva, Herman; Rosyara, Umesh R.; Iezzoni, Amy; Meisel, Lee A.; Dirlewanger, Elisabeth.

En: PLoS ONE, Vol. 8, N.º 1, e54743, 31.01.2013.

Resultado de la investigación: Contribución a la publicaciónArticle

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AU - Guzmán,Alejandra

AU - Mansur,Levi

AU - Gratacós,Eduardo

AU - Silva,Herman

AU - Rosyara,Umesh R.

AU - Iezzoni,Amy

AU - Meisel,Lee A.

AU - Dirlewanger,Elisabeth

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Klagges C, Campoy JA, Quero-García J, Guzmán A, Mansur L, Gratacós E y otros. Construction and Comparative Analyses of Highly Dense Linkage Maps of Two Sweet Cherry Intra-Specific Progenies of Commercial Cultivars. PLoS ONE. 2013 ene 31;8(1). e54743. Disponible desde, DOI: 10.1371/journal.pone.0054743