Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development

María Isabel Oliver, Carla Rodríguez, Paula Bustos, Violeta Morín, Soraya Gutierrez, Martín Montecino, Anne Marie Genevière, Marcia Puchi, María Imschenetzky

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

Three sets of histone variants are coexisting in the embryo at larval stages of sea urchin's development: the maternally inherited cleavage stage variants (CS) expressed during the two initial cleavage divisions, the early histone variants, which are recruited into embryonic chromatin from middle cleavage stages until hatching and the late variants, that are fundamentally expressed from blastula stage onward. Since the expression of the CS histones is confined to the initial cleavage stages, these variants represent a very minor proportion of the histones present in the plutei larvae, whereas the late histone variants are predominant. To determine the position of these CS in the embryonic territories, we have immunolocalized the CS histone variants in plutei larvas harvested 72 h post-fertilization. In parallel, we have pulse labeled the DNA replicated during the initial cleavage cycle with bromodeoxyuridine (BrdU) and its position was further determined in the plutei larvas by immunofluorescence. We have found that the CS histone variants were segregated to specific territories in the plutei. The position in which the CS histone variants were found to be segregated was consistent with the position in which the DNA molecules that were replicated during the initial cleavage divisions were localized. These results strongly suggest that a specification of embryonic nuclei occurs at the initial cleavage divisions which is determined by a chromatin organized by CS histone variants.

Idioma originalEnglish
Páginas (desde-hasta)643-649
Número de páginas7
PublicaciónJournal of Cellular Biochemistry
Volumen88
N.º4
DOI
EstadoPublished - 1 mar 2003

Huella dactilar

Sea Urchins
Histones
Larva
Chromatin
Blastula
Maternal Inheritance
DNA
Bromodeoxyuridine
Fertilization
Fluorescent Antibody Technique
Embryonic Structures
Specifications
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

Oliver, María Isabel ; Rodríguez, Carla ; Bustos, Paula ; Morín, Violeta ; Gutierrez, Soraya ; Montecino, Martín ; Genevière, Anne Marie ; Puchi, Marcia ; Imschenetzky, María. / Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development. En: Journal of Cellular Biochemistry. 2003 ; Vol. 88, N.º 4. pp. 643-649.
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abstract = "Three sets of histone variants are coexisting in the embryo at larval stages of sea urchin's development: the maternally inherited cleavage stage variants (CS) expressed during the two initial cleavage divisions, the early histone variants, which are recruited into embryonic chromatin from middle cleavage stages until hatching and the late variants, that are fundamentally expressed from blastula stage onward. Since the expression of the CS histones is confined to the initial cleavage stages, these variants represent a very minor proportion of the histones present in the plutei larvae, whereas the late histone variants are predominant. To determine the position of these CS in the embryonic territories, we have immunolocalized the CS histone variants in plutei larvas harvested 72 h post-fertilization. In parallel, we have pulse labeled the DNA replicated during the initial cleavage cycle with bromodeoxyuridine (BrdU) and its position was further determined in the plutei larvas by immunofluorescence. We have found that the CS histone variants were segregated to specific territories in the plutei. The position in which the CS histone variants were found to be segregated was consistent with the position in which the DNA molecules that were replicated during the initial cleavage divisions were localized. These results strongly suggest that a specification of embryonic nuclei occurs at the initial cleavage divisions which is determined by a chromatin organized by CS histone variants.",
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Oliver, MI, Rodríguez, C, Bustos, P, Morín, V, Gutierrez, S, Montecino, M, Genevière, AM, Puchi, M & Imschenetzky, M 2003, 'Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development', Journal of Cellular Biochemistry, vol. 88, n.º 4, pp. 643-649. https://doi.org/10.1002/jcb.10416

Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development. / Oliver, María Isabel; Rodríguez, Carla; Bustos, Paula; Morín, Violeta; Gutierrez, Soraya; Montecino, Martín; Genevière, Anne Marie; Puchi, Marcia; Imschenetzky, María.

En: Journal of Cellular Biochemistry, Vol. 88, N.º 4, 01.03.2003, p. 643-649.

Resultado de la investigación: Article

TY - JOUR

T1 - Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development

AU - Oliver, María Isabel

AU - Rodríguez, Carla

AU - Bustos, Paula

AU - Morín, Violeta

AU - Gutierrez, Soraya

AU - Montecino, Martín

AU - Genevière, Anne Marie

AU - Puchi, Marcia

AU - Imschenetzky, María

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Three sets of histone variants are coexisting in the embryo at larval stages of sea urchin's development: the maternally inherited cleavage stage variants (CS) expressed during the two initial cleavage divisions, the early histone variants, which are recruited into embryonic chromatin from middle cleavage stages until hatching and the late variants, that are fundamentally expressed from blastula stage onward. Since the expression of the CS histones is confined to the initial cleavage stages, these variants represent a very minor proportion of the histones present in the plutei larvae, whereas the late histone variants are predominant. To determine the position of these CS in the embryonic territories, we have immunolocalized the CS histone variants in plutei larvas harvested 72 h post-fertilization. In parallel, we have pulse labeled the DNA replicated during the initial cleavage cycle with bromodeoxyuridine (BrdU) and its position was further determined in the plutei larvas by immunofluorescence. We have found that the CS histone variants were segregated to specific territories in the plutei. The position in which the CS histone variants were found to be segregated was consistent with the position in which the DNA molecules that were replicated during the initial cleavage divisions were localized. These results strongly suggest that a specification of embryonic nuclei occurs at the initial cleavage divisions which is determined by a chromatin organized by CS histone variants.

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KW - Echinoderms

KW - Embryogenesis

KW - Histones

KW - Nucleus

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