Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development

María Isabel Oliver, Carla Rodríguez, Paula Bustos, Violeta Morín, Soraya Gutierrez, Martín Montecino, Anne Marie Genevière, Marcia Puchi, María Imschenetzky

Resultado de la investigación: Contribución a una revistaArtículo

3 Citas (Scopus)

Resumen

Three sets of histone variants are coexisting in the embryo at larval stages of sea urchin's development: the maternally inherited cleavage stage variants (CS) expressed during the two initial cleavage divisions, the early histone variants, which are recruited into embryonic chromatin from middle cleavage stages until hatching and the late variants, that are fundamentally expressed from blastula stage onward. Since the expression of the CS histones is confined to the initial cleavage stages, these variants represent a very minor proportion of the histones present in the plutei larvae, whereas the late histone variants are predominant. To determine the position of these CS in the embryonic territories, we have immunolocalized the CS histone variants in plutei larvas harvested 72 h post-fertilization. In parallel, we have pulse labeled the DNA replicated during the initial cleavage cycle with bromodeoxyuridine (BrdU) and its position was further determined in the plutei larvas by immunofluorescence. We have found that the CS histone variants were segregated to specific territories in the plutei. The position in which the CS histone variants were found to be segregated was consistent with the position in which the DNA molecules that were replicated during the initial cleavage divisions were localized. These results strongly suggest that a specification of embryonic nuclei occurs at the initial cleavage divisions which is determined by a chromatin organized by CS histone variants.

Idioma originalInglés
Páginas (desde-hasta)643-649
Número de páginas7
PublicaciónJournal of Cellular Biochemistry
Volumen88
N.º4
DOI
EstadoPublicada - 1 mar 2003

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología celular
  • Biología molecular

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    Oliver, M. I., Rodríguez, C., Bustos, P., Morín, V., Gutierrez, S., Montecino, M., Genevière, A. M., Puchi, M., & Imschenetzky, M. (2003). Conservative segregation of maternally inherited CS histone variants in larval stages of sea urchin development. Journal of Cellular Biochemistry, 88(4), 643-649. https://doi.org/10.1002/jcb.10416