Conformational Changes of Enzymes and Aptamers Immobilized on Electrodes

Alejandra Tello, Roberto Cao, María José Marchant, Humberto Gomez

Resultado de la investigación: Review article

13 Citas (Scopus)

Resumen

Conformation constitutes a vital property of biomolecules, especially in the cases of enzymes and aptamers, and is essential in defining their molecular recognition ability. When biomolecules are immobilized on electrode surfaces, it is very important to have a control on all the possible conformational changes that may occur, either upon the recognition of their targets or by undesired alterations. Both enzymes and aptamers immobilized on electrodes are susceptible to conformational changes as a response to the nature of the charge of the surface and of the surrounding environment (pH, temperature, ionic strength, etc.). The main goal of this review is to analyze how the conformational changes of enzymes and aptamers immobilized on electrode surfaces have been treated in reports on biosensors and biofuel cells. This topic was selected due to insufficient information found on the actual conformational changes involved in the function of these bioelectrochemical devices despite its importance.

Idioma originalEnglish
Páginas (desde-hasta)2581-2591
Número de páginas11
PublicaciónBioconjugate Chemistry
Volumen27
N.º11
DOI
EstadoPublished - 16 nov 2016
Publicado de forma externa

Huella dactilar

Immobilized Enzymes
Electrodes
Enzymes
Biomolecules
Bioelectric Energy Sources
Biological fuel cells
Molecular recognition
Biosensing Techniques
Ionic strength
Biosensors
Osmolar Concentration
Conformations
Equipment and Supplies
Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Citar esto

Tello, Alejandra ; Cao, Roberto ; Marchant, María José ; Gomez, Humberto. / Conformational Changes of Enzymes and Aptamers Immobilized on Electrodes. En: Bioconjugate Chemistry. 2016 ; Vol. 27, N.º 11. pp. 2581-2591.
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Conformational Changes of Enzymes and Aptamers Immobilized on Electrodes. / Tello, Alejandra; Cao, Roberto; Marchant, María José; Gomez, Humberto.

En: Bioconjugate Chemistry, Vol. 27, N.º 11, 16.11.2016, p. 2581-2591.

Resultado de la investigación: Review article

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