Computational studies of the metal-binding site of the wild-type and the H46R mutant of the copper, zinc superoxide dismutase

Rauìl Mera-Adasme, Fernando Mendizábal, Mauricio Gonzalez, Sebastián Miranda-Rojas, Claudio Olea-Azar, Dage Sundholm

Resultado de la investigación: Contribución a una revistaArtículo

10 Citas (Scopus)

Resumen

Impairment of the Zn(II)-binding site of the copper, zinc superoxide dismutase (CuZnSOD) protein is involved in a number of hypotheses and explanations for the still unknown toxic gain of function mutant varieties of CuZnSOD that are associated with familial forms of amyotrophic lateral sclerosis (ALS). In this work, computational chemistry methods have been used for studying models of the metal-binding site of the ALS-linked H46R mutant of CuZnSOD and of the wild-type variety of the enzyme. By comparing the energy and electronic structure of these models, a plausible explanation for the effect of the H46R mutation on the zinc site is obtained. The computational study clarifies the role of the D124 and D125 residues for keeping the structural integrity of the Zn(II)-binding site, which was known to exist but its mechanism has not been explained. Earlier results suggest that the explanation for the impairment of the Zn(II)-site proposed in this work may be useful for understanding the mechanism of action of the ALS-linked mutations in CuZnSOD in general.

Idioma originalInglés
Páginas (desde-hasta)5561-5568
Número de páginas8
PublicaciónInorganic Chemistry
Volumen51
N.º10
DOI
EstadoPublicada - 21 may 2012

Áreas temáticas de ASJC Scopus

  • Química física y teórica
  • Química inorgánica

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