Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes

Talía Del Pozo, Simón Miranda, Mauricio Latorre, Felipe Olivares, Leonardo Pavez, Ricardo Gutiérrez, Jonathan Maldonado, Patricio Hinrichsen, Bruno G. Defilippi, Ariel Orellana, Mauricio González

Resultado de la investigación: Article

Resumen

Cold storage of fruit is one of the methods most commonly employed to extend the postharvest lifespan of peaches (Prunus persica (L.) Batsch). However, fruit quality in this species is affected negatively by mealiness, a physiological disorder triggered by chilling injury after long periods of exposure to low temperatures during storage and manifested mainly as a lack of juiciness, which ultimately modifies the organoleptic properties of peach fruit. The aim of this study was to identify molecular components and metabolic processes underlying mealiness in susceptible and nonsusceptible segregants. Transcriptome and qRT-PCR profiling were applied to individuals with contrasting juiciness phenotypes in a segregating F 2 population. Our results suggest that mealiness is a multiscale phenomenon, because juicy and mealy fruit display distinctive reprogramming processes affecting translational machinery and lipid, sugar, and oxidative metabolism. The candidate genes identified may be useful tools for further crop improvement.

Idioma originalEnglish
Páginas (desde-hasta)1598-1607
Número de páginas10
PublicaciónJournal of Agricultural and Food Chemistry
Volumen67
N.º5
DOI
EstadoPublished - 6 feb 2019

Huella dactilar

juiciness
Gene Expression Profiling
Fruits
transcriptomics
peaches
Fruit
Phenotype
phenotype
fruits
Population
chilling injury
aerobiosis
Prunus persica
Cold storage
Chilling
cold storage
transcriptome
storage temperature
exposure duration
fruit quality

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Del Pozo, T., Miranda, S., Latorre, M., Olivares, F., Pavez, L., Gutiérrez, R., ... González, M. (2019). Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes. Journal of Agricultural and Food Chemistry, 67(5), 1598-1607. https://doi.org/10.1021/acs.jafc.8b05177
Del Pozo, Talía ; Miranda, Simón ; Latorre, Mauricio ; Olivares, Felipe ; Pavez, Leonardo ; Gutiérrez, Ricardo ; Maldonado, Jonathan ; Hinrichsen, Patricio ; Defilippi, Bruno G. ; Orellana, Ariel ; González, Mauricio. / Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes. En: Journal of Agricultural and Food Chemistry. 2019 ; Vol. 67, N.º 5. pp. 1598-1607.
@article{0e6741974bb14f3a8260bd7fff116e6e,
title = "Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes",
abstract = "Cold storage of fruit is one of the methods most commonly employed to extend the postharvest lifespan of peaches (Prunus persica (L.) Batsch). However, fruit quality in this species is affected negatively by mealiness, a physiological disorder triggered by chilling injury after long periods of exposure to low temperatures during storage and manifested mainly as a lack of juiciness, which ultimately modifies the organoleptic properties of peach fruit. The aim of this study was to identify molecular components and metabolic processes underlying mealiness in susceptible and nonsusceptible segregants. Transcriptome and qRT-PCR profiling were applied to individuals with contrasting juiciness phenotypes in a segregating F 2 population. Our results suggest that mealiness is a multiscale phenomenon, because juicy and mealy fruit display distinctive reprogramming processes affecting translational machinery and lipid, sugar, and oxidative metabolism. The candidate genes identified may be useful tools for further crop improvement.",
keywords = "cold storage, gene expression, mealiness, Prunus persica, transcriptome",
author = "{Del Pozo}, Tal{\'i}a and Sim{\'o}n Miranda and Mauricio Latorre and Felipe Olivares and Leonardo Pavez and Ricardo Guti{\'e}rrez and Jonathan Maldonado and Patricio Hinrichsen and Defilippi, {Bruno G.} and Ariel Orellana and Mauricio Gonz{\'a}lez",
year = "2019",
month = "2",
day = "6",
doi = "10.1021/acs.jafc.8b05177",
language = "English",
volume = "67",
pages = "1598--1607",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "5",

}

Del Pozo, T, Miranda, S, Latorre, M, Olivares, F, Pavez, L, Gutiérrez, R, Maldonado, J, Hinrichsen, P, Defilippi, BG, Orellana, A & González, M 2019, 'Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes', Journal of Agricultural and Food Chemistry, vol. 67, n.º 5, pp. 1598-1607. https://doi.org/10.1021/acs.jafc.8b05177

Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes. / Del Pozo, Talía; Miranda, Simón; Latorre, Mauricio; Olivares, Felipe; Pavez, Leonardo; Gutiérrez, Ricardo; Maldonado, Jonathan; Hinrichsen, Patricio; Defilippi, Bruno G.; Orellana, Ariel; González, Mauricio.

En: Journal of Agricultural and Food Chemistry, Vol. 67, N.º 5, 06.02.2019, p. 1598-1607.

Resultado de la investigación: Article

TY - JOUR

T1 - Comparative Transcriptome Profiling in a Segregating Peach Population with Contrasting Juiciness Phenotypes

AU - Del Pozo, Talía

AU - Miranda, Simón

AU - Latorre, Mauricio

AU - Olivares, Felipe

AU - Pavez, Leonardo

AU - Gutiérrez, Ricardo

AU - Maldonado, Jonathan

AU - Hinrichsen, Patricio

AU - Defilippi, Bruno G.

AU - Orellana, Ariel

AU - González, Mauricio

PY - 2019/2/6

Y1 - 2019/2/6

N2 - Cold storage of fruit is one of the methods most commonly employed to extend the postharvest lifespan of peaches (Prunus persica (L.) Batsch). However, fruit quality in this species is affected negatively by mealiness, a physiological disorder triggered by chilling injury after long periods of exposure to low temperatures during storage and manifested mainly as a lack of juiciness, which ultimately modifies the organoleptic properties of peach fruit. The aim of this study was to identify molecular components and metabolic processes underlying mealiness in susceptible and nonsusceptible segregants. Transcriptome and qRT-PCR profiling were applied to individuals with contrasting juiciness phenotypes in a segregating F 2 population. Our results suggest that mealiness is a multiscale phenomenon, because juicy and mealy fruit display distinctive reprogramming processes affecting translational machinery and lipid, sugar, and oxidative metabolism. The candidate genes identified may be useful tools for further crop improvement.

AB - Cold storage of fruit is one of the methods most commonly employed to extend the postharvest lifespan of peaches (Prunus persica (L.) Batsch). However, fruit quality in this species is affected negatively by mealiness, a physiological disorder triggered by chilling injury after long periods of exposure to low temperatures during storage and manifested mainly as a lack of juiciness, which ultimately modifies the organoleptic properties of peach fruit. The aim of this study was to identify molecular components and metabolic processes underlying mealiness in susceptible and nonsusceptible segregants. Transcriptome and qRT-PCR profiling were applied to individuals with contrasting juiciness phenotypes in a segregating F 2 population. Our results suggest that mealiness is a multiscale phenomenon, because juicy and mealy fruit display distinctive reprogramming processes affecting translational machinery and lipid, sugar, and oxidative metabolism. The candidate genes identified may be useful tools for further crop improvement.

KW - cold storage

KW - gene expression

KW - mealiness

KW - Prunus persica

KW - transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85061126200&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.8b05177

DO - 10.1021/acs.jafc.8b05177

M3 - Article

C2 - 30632375

AN - SCOPUS:85061126200

VL - 67

SP - 1598

EP - 1607

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 5

ER -